| Topic | Lecturer | ||
| 1.
Genetic diversity - Sources of genetics variation | Prof. Wicki | ||
| 2.
Sampling strategy - Types of tissues, pre-handling of samples, protein extraction, DNA extraction | Prof.
Wicki | ||
| 3.
Protein extraction Isozymes - enzyme systems, electrophoretic methods (PAGE, CAE, starch gel). Estimation of population genetic parameters viz. allele frequency, heterozygosity, inbreeding coefficient. Practical 1 - Isozyme analysis | Prof. Wicki | ||
| 4. DNA
extraction - Sources of DNA (nuclear DNA, chloroplast DNA, mitochondrial DNA), characteristics of genome | Prof. Wicki | ||
Mid-Semester Assessment: 30% | |||
| 5.
DNA polymorphism I Non-PCR based techniques using restriction enzymes and DNA probes e.g. RFLP Note I Note II Practical 2 - RFLP analysis | Dr. Choong | ||
| 6. DNA
polymorphism II Note I Note II Note III PCR based techniques using partially specific primers e.g. RADP, AFLP Practical 3 - RAPD analysis | Dr. Choong | ||
| 7. DNA
polymorphism III Note I Note II PCR based techniques using specific primers e.g. CAPS, SSR | Dr. Choong | ||
| 8.
Molecular data analysis - genetic distance, genetic diversity within and among populations or species, mating system | Prof.
Wicki | ||
| 9. Applications of molecular markers in in situ and ex situ conservation | Prof.
Wicki | ||
| Practicals
1, 2 and 3: 15% Final Examination: 55% |