Sains Ma1aysiana 25(1): 77-86 (1996)                                                                                                   Sains Hayat/

                                                                                                                                                         Life Sciences

 

Levels of Malondialdehyde-Related Immune

Reactants in a Lupus Prone Mouse Strain

 

 

M. Danial Yahya

Jabatan Mikrobiologi

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E. Malaysia

 

Chien C. Lung & Yacob L. Pinnas

Dept. of Medicine, Arizona Health Science Center

Tucson, AZ 85724, USA

 

 

ABSTRACT

 

The levels of malondialdehyde (MDA)-specific antibodies in lupus-prone MRL/ lpr mice of different ages were compared to those against DNA. These mice elicited anti-MDA antibodies earlier and in higher levels that anti-DNA antibodies. The levels of immune complexes containing MDA adducts were also higher in these mice when compared to 3 other non-lupus strains. MDA binding to a 100 kDa serum protein was observed in 3 and 5 month old mice. Immune complexes involving anti-MDA antibodies and MDA adducts may represent an additional mechanism that contributes to disease pathogenesis in these mice.

 

ABSTRAK

 

Aras antibodi anti-malondialdehida (MDA) dalam mencit MRL/lpr berbeza umur telah dibandingkan dengan aras antibodi anti-DNA. Mencit ini menghasilkan antibodi anti-MDA lebih awal dan pada aras yang lebih tinggi berbanding antibodi anti-DNA. Aras kompleks imun yang mengandungi MDA juga lebih tinggi berbanding aras dalam 3 strain mencit lain. Pergabungan MDA dengan suatu protein serum bersaiz 100 kDa dikesan pada mencit berumur 3 dan 5 bulan. Kompleks imun yang mengandungi MDA dan antibodi anti-MDA mungkin merupakan satu lagi mekanisma yang terlibat dalam patogenesis penyakit lupus pada mencit strain ini.

 

RUJUKAN/REFERENCES

 

EY, P. L., Prowse, S. J. & Jenkin, C.R. 1978. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Biochem. 15: 429-436.

Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.

Leary, J. J., Brigati, D. J. & Ward, D.C. 1983. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc. Natl. Acad. Sci. USA 80: 4045-4049.

Lung, C. C., Pinnas, J. L., Yahya, M. D., Meinke, G. C. & Mooradian, A. D. 1993. Malondialdehyde-modified protein and their antibodies plasma of control and streptozotocin-induced diabetic rats. Life Sci. 52: 329-337.

Lung, C. C., Fleisher, J. F., Meinke, G. & Pinnas, J. L. 1990. Immunochemical properties of malondialdehyde-protein adducts. J. Immunol. Methods 128: 127-132. 

Mark, D. A., Alonso, D. R., Quimby, F., Thaler, H. T., Kim, Y. T., Fernandes, G., Good, R. A. & Weksler, M. E. 1984. Effects of nutrition on disease and lifespan. I. Immune responses, cardiovascular pathology, and lifespan in MRL mice. Am. J. Pathol. 117(1): 110-124.

Mooradian, A. D., Pinnas, J. L., Lung, C. C., Yahya, M. D. & Meredith, K. 1994. Diabetes-related changes in the protein composition of rat cerebral microvessels. Neurochem. Res. 19(2): 123-128.

Portanova, J. P., Arndt, R. E., & Kotzin, B. (1988). Selective production of autoantibodies in cronic graft-versus-host-host-induced and spontaneous murine lupus. Predominant reactivity with histone regions accessible in chromatin. J. Immunol. 140(3): 755-760.

Salmon, S., Maziere, C., Theron, L., Beucler, I., Ayrault-Jarrier, M., Goldstein, S. & Polonovski, J. 1987. Immunological detection of low density lipoproteins modified by malondialdehyde in vitro or in vivo. Biochim. Biophys. Acta 920: 215-220.

Theofilopoulos, A. N. & Dixon, F. J. 1985. Murine models of systemic lupus erythematosus. Adv. Immunol. 37: 269-390.       

Towbin, H., Staehelin, T. & Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.

Yahya, M. D. & Pinnas, J. L. 1993. Alterations in the physicochemical and antigenic properties of albumin resulting from malondialdehyde binding. Malays. Appl. Bio. 22(1): 1-13.

 

 

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