Sains Malaysiana 37(2):  131-135(2008)

Surface Analysis of Marine Sulphate-Reducing Bacteria

Exopolymers on Steel During Biocorrosion Using X-ray

Photoelectron Spectroscopy

(Analisis Permukaan Eksopolimer Bakteria Penurun-Sulfat Marin pada Keluli

Semasa Kakisan Bio Menggunakan Spektroskopi Fotoelektron Sinar-X)

Fathul Karim Sahrani

Pusat Pengajian Sains Sekitaran dan Sumber Alam

Fakulti Sains dan Teknologi 43600 UKM Bangi,

Selangor Darul Ehsan, Malaysia

Madzlan Abd. Aziz, Zaharah Ibrahim & Adibah Yahya

Jabatan Kimia / Biologi, Fakulti Sains, 81310 UTM Skudai,

 Johor Darul Takzim, Malaysia

Received:  25 July 2007 / Accepted:  25 September 2007

ABSTRAK

Tujuan kajian ini adalah untuk menentukan keadaan kimia permukaan semasa proses kakisan bio terhadap pertumbuhan dan penghasilan bahan eksopolimer (EPS) dalam campuran pengkulturan bakteria penurun sulfat marin yang dipencilkan daripada Kejuruteraan Berat dan Marin Malaysia Sdn. Bhd. (MHHE), Pasir Gudang. EPS dan mendakan pada permukaan keluli dianalisis menggunakan spektroskopi fotoelektron sinar-x (XPS). Analisis menggunakan XPS menunjukkan spektrum  Fe(2p_3/2) untuk besi sulfida boleh dipadankan kepada komponen Fe(II) dan Fe(III), sepadan kepada jenis ikatan Fe-S. Ketiadaan oksigen oksida dalam spektrum O(1s) dan jenis ikatan Fe(III)-O dalam spektrum Fe(2p_3/2) menyokong kesimpulan bahawa besi sulfida mengandungi kedua-dua besi ferik dan ferus serupa dengan monosulfida dan disulfida.

Kata kunci: bakteria penurun-sulfat; kakisan bio; bahan eksopolimer; spektroskopi fotoelektron sinar-x.

ABSTRACT

The aim of this study was to determine the surface chemistry during biocorrosion process on growth and on the production of exopolymeric substances (EPS) in batch cultures of mix-strains of marine sulphate-reducing bacteria (SRB) isolated from Malaysian Shipyard and Engineering Harbours, Pasir Gudang. The EPS and precipitates were analyzed by x-ray photoelectron spectroscopy (XPS).  The XPS results indicate that Fe(2p_3/2) spectrum for iron sulphide can be fitted with Fe(II) and Fe(III) components, both corresponding to Fe-S bond types. The absence of oxide oxygen in the O(1s) spectrum and Fe(III)-O bond types in the Fe(2p_3/2) spectrum supports the conclusion that iron sulphides are composed of both ferric and ferrous iron coordinated with monosulphide and disulphide.

Keywords: sulphate-reducing bacteria; biocorrosion; exopolymeric substances; x-ray photoelectron spectroscopy.

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