Sains Malaysiana 37(4):  429-433(2008)

 

Peningkatan Produktiviti CGtase dengan Menggunakan

Kultur Selanjar

(Increased productivity of CGTase using continuous culture)

 

 

Khosiya Sali, Aidil Abdul Hamid & Wan Mohtar Wan Yusoff 

Program Mikrobiologi, Pusat pengajian Biosains dan Bioteknologi

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor, Malaysia

 

 

Diserahkan:  12 April 2007 / Diterima: 14 Disember 2008

 

 

ABSTRAK

Penghasilan CGTase daripada Bacillus sp. G1 berjaya ditingkatkan dengan menggunakan sistem kultur selanjar mengatasi penghasilan daripada kultur kelompok.  Aktiviti CGTase tertinggi yang didapati dalam kultur kelompok ialah 28.1 U/ml.  Kajian kultur selanjar difokuskan kepada masa kemasukan medium segar yang berbeza (12, 24 dan 48 jam ), kadar pencairan ditetapkan pada 0.03 per jam.  Hasil menunjukkan masa memulakan pam medium segar tidak memberi perubahan yang signifikan terhadap aktiviti CGTase (25.7, 26.3 dan 26.1 U/ml masing-masing) dan produktiviti CGTase (0.77, 0.79 dan 0.78 U/ml/j masing-masing) pada keadaan mantap tetapi produktiviti CGTase (0.77 U/ml/j) akan lebih tinggi berbanding produktiviti kultur kelompok apabila masa di antara larian kultur kelompok diambil kira. Malah peningkatan berpotensi ditingkatkan lagi dengan memulakan pam medium segar lebih awal daripada 12 jam dan juga dengan meningkatkan kadar pencairan.

 

Kata kunci: Bacillus sp. G1; CGTase; kultur selanjar

 

 

ABSTRACT

 

The production of CGTase from Bacillus sp. G1 was successfully increased using continuous culture.  The highest CGTase activity in batch culture was 28.1 U/ml.  The study in continuous culture was focused on the effect of different time (12, 24 and 48 hours) to start pumping fresh medium at fixed dilution rate of 0.03 per hour.  Results showed that the pumping time did not give significant changes in CGTase activity (25.7, 26.3 and 26.1 U/ml respectively) and in CGTase productivity (0.77, 0.78 and 0.78 U/ml/h) respectively at steady state operation.  Nevertheless, the productivity (0.77 U/ml/h) of CGTase was found to be higher than that obtained in batch culture when the time interval between batch run was taken into account.  The productivity could be further increased by pumping the media earlier than 12 hours and also by increasing the dilution rate.

 

Keyword:  Bacillus sp. G1; CGTase; continuous culture

 

 

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