Sains Malaysiana 39(1)(2010): 141–144

 

Kesan Pengudaraan dan Pencairan ke Atas Penghasilan Bioetanol

Secara Selanjar dalam Bioreaktor Padat oleh Saccharomyces cerevisiae

(Effect of Aeration and Dilution on Continuous Bioethanol Production  in a Packed-bed Bioreactor by Saccharomyces cerevisiae)

 

Ng Kee Wee, Aidil Abdul Hamid, Mohd Sahaid Kalil & Wan Mohtar Wan Yusoff*

Pusat Pengajian Biosains dan Bioteknologi

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E., Malaysia

 

Received: 12 June 2008 / Accepted: 6 July 2009

 

 

ABSTRAK

 

Pertumbuhan sel secara selanjar pada keadaan mantap telah diperolehi dalam pengkulturan dengan bioreaktor padat. Produktiviti (0.02 g/L/j) bioetanol yang paling tinggi diperolehi adalah pada pengudaraan 0.003 vvm. Produktiviti bioetanol didapati meningkat dengan peningkatan kadar pencairan. Produktiviti tertinggi sebanyak 0.037 g/L/j direkod semasa kadar pencairan (D) 0.05 per jam. Penghasilan bioetanol secara selanjar telah berjaya diselenggarakan pada keadaan tidak 100% anaerobik. Pengudaraan yang terbaik untuk produktiviti bioetanol dalam keadaan seimbang pertumbuhan sel dan penghasilan bioetanol ialah pada 0.003 vvm.

 

Kata kunci: bioetanol; Saccharomyces cerevisiae; sel terperangkap

 

ABSTRACT

 

Stability of cell growth was achieved continuously at a steady state in a packed-bed bioreactor. The highest productivity of ethanol was achieved (0.02 g/L/h) when 0.003 vvm was employed. The productivity of bioethanol increases when dilution rate increases. The highest production of 0.037 g/L/h was recorded when the dilution rate (D) was at 0.05 per hour. The production of bioethanol was sucessfully maintained in a non 100% anaerobic condition. The best aeration for the continous production of bioethanol in a condition of steady state growth was at an aeration rate of 0.003 vvm.

 

Keywords: Bioethanol; entraped cell; Saccharomyces cerevisiae

 

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*Corresponding author; email: wantar@ukm.my

 

 

 

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