Sains Malaysiana 40(11)(2011): 1319–1323

Altered Levels of Serum Haptoglobin and Apo A-I in Schizophrenia

(Perubahan Paras Haptoglobin dan Apo A-I Serum dalam Skizofrenia)

Tze Jen Chow & Han Chern Loh*

Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Jalan Genting Kelang,

Setapak 53300 Kuala Lumpur, Malaysia

Received: 16 December 2010 / Accepted: 22 February 2011

ABSTRACT

Schizophrenia is a debilitating psychiatric syndrome that affects 1% of the world’s population. Abnormalities in immune functions and role of inflammatory markers have been widely described in schizophrenia. With the alarming high prevalence, this study aims to investigate the association of acute phase proteins with schizophrenia. We investigated the serum proteome of 20 schizophrenic patients and 20 healthy controls using two-dimensional gel electrophoresis and mass spectrometry. The spots were analysed using Image Master 2D Platinum software. In total, we have detected 774 protein spots in human serum and found that three of them showed altered changes in schizophrenic patients, as compared to healthy controls. Among these acute phase proteins, haptoglobin (p = 0.003) and two isoforms of apolipoprotein A-I (p = 0.004, p = 0.003) were found to be significantly decreased in patients, as compared to controls. Our findings support the hypothesis that inflammatory response system is linked to the pathophysiology of schizophrenia.

Keywords: Apolipoprotein A-I; haptoglobin; schizophrenia

ABSTRAK

Skizofrenia adalah sejenis penyakit psikiatrik yang melesukan dan mempengaruhi 1% daripada penduduk sedunia. Ketidaknormalan dalam fungsi pertahanan dan peranan sebagai penanda keradangan semakin meluas dihuraikan dalam skizofrenia. Dengan bahaya yang berpeluang tinggi dijangkiti, kajian ini bertujuan untuk menyelidik hubungan protein fasa tajam dengan skizofrenia. Kami mengkaji proteom serum 20 pesakit skizofrenia dan 20 kawalan yang sihat dengan menggunakan gel elektroforesis dua-dimensi dan spektrometri jisim. Tanda-tanda dianalisiskan dengan menggunakan perisian ImageMaster 2D Platinum. Secara kesuluruhannya, kami telah mengesan 774 tanda protein dalam serum manusia dan mendapati tiga tanda menunjukkan perubahan dalam pesakit skizofrenia berbandingkan dengan kawalan yang sihat. Di kalangan tiga protein fasa tajam ini, haptoglobin (p = 0.003) dan dua isofom apolipoprotein A-I (p = 0.004, p = 0.003) didapati menurun secara nyata dalam pesakit berbanding dengan kawalan. Keputusan ini menyokong andaian bahawa sistem tindak balas keradangan adalah berhubung kepada patofisiologi skizofrenia.

Kata kunci: Apolipoprotein A-I; haptoglobin; skizofrenia

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*Corresponding author; email: hcloh@utar.edu.my