Sains Malaysiana 41(2)(2012): 149–154

 

Clonal Diversity and Genetic Differentiation in Rhizomatous Herb, Iris japonica (Iridaceae) Populations on Jinyun Mountain, Southwest China

(Kepelbagaian Klon dan Pembezaan Genetik dalam Populasi Tumbuhan Berizom,

Iris japonica (Iridaceae) di Gunung Jinyun, Barat Daya China)

 

 

Yong-Jian Wang*

College of Horticulture & Forestry Sciences, Huazhong Agricultural University

Wuhan 430070 China

 

Xue-Ping Shi

College of Horticulture & Forestry Sciences, Huazhong Agricultural University

Wuhan 430070 China

 

Zhang-Cheng Zhong

Key Laboratory of Eco-environments of Three Gorges Reservoir Region

(Ministry of Education) of Southwest University

Chongqing 400715 China

 

Received: 31 May 2011 / Accepted: 1 August 2011

 

 

ABSTRACT

Many plants can reproduce both clonally and sexually, and the relative importance between the two modes of reproduction varied among habitats. Clonal diversity and genetic differentiation of the rhizomatous herb, Iris japonica populations, was analyzed by 12 randomly amplified polymorphic DNA markers to determine the extent that reproductive mode varied locally between two habitats, open area of forest edge (OAFE, 2 populations OAFE 1 and OAFE 2) and bamboo forest (BF, 2 populations BF 1 and BF 2) on Jinyun Mountain, southwest China. Total clonal diversity and genetic diversity of I. japonica populations were high. The clonal diversity and genetic diversity of I. japonica populations in BF habitats were lower than those in OAFE habitats. Neighbor-joining tree by Jaccard’s genetic distance showed two genetically distinct groups: OAFE group and BF group. AMOVA indicated that about half of the total variation existed within the four populations, and that genetic differentiation among habitats (BF populations and OAFE populations) was also significant. OAFE habitats were helpful in maintaining clonal diversity and genetic diversity of I. japonica populations than BF habitats. Habitat differences might have distinguished effects on the relative significance of clonal propagation and sexual reproduction, and further have a fatal influence on clonal diversity, genetic diversity and genetic differentiation of I. japonica populations in Jinyun Mountain, Southwest China.

 

Keywords: Clonal diversity; genetic diversity; Iris japonica; population differentiation; RAPD

 

 

ABSTRAK

Banyak tumbuhan boleh membiak secara klon dan seksual, dan kepentingan relatif di antara kedua-dua cara pembiakan berbeza-beza mengikut habitat. Kepelbagaian klon dan pembezaan genetik bagi populasi tumbuhan berizom, Iris japonica, dianalisis dengan 12 penanda polimorfik DNA teramplikasi rawak untuk menentukan perbezaan setempat cara pembiakan antara dua habitat: kawasan terbuka pinggir hutan (OAFE; 2 populasiOAFE 1 dan OAFE 2) dan hutan buluh (BF; 2 populasiBF 1 dan BF 2) di Gunung Jinyun, barat daya China. Kepelbagaian total klon dan genetik bagi populasiI. japonica adalah tinggi. Kepelbagian klon dan genetik bagi populasi BF adalah lebih rendah berbanding populasi OAFE. Pohon Neighbor-joining berdasarkan jarak genetik Jaccard menunjukkan kewujudan dua kumpulan genetik yang berbeza: kumpulan OAFE dan kumpulan BF. AMOVA menunjukkan separuh daripada variasi terkandung dalam empat populasi, dan pembezaan genetik antara habitat (populasi BF dan populasi OAFE) adalah signifikan. Habitat OAFE lebih berguna dalam pengekalan kepelbagaian klon dan genetik populasi I. japonica jika dibandingkan dengan habitat BF. Perbezaan habitat mungkin mempunyai kesan membezakan yang relatif signifikan pada propagasi klon dan pembiakan seksual, dan ini seterusnya memberikan kesan kemandirian pada kepelbagian klon, kepelbagaian genetik dan pembezaan genetik I. japonica di Gunung Jinyun, barat daya China.

 

Kata kunci: Iris japonica; kepelbagaian genetik; kepelbagaian klon; pembezaan populasi; RAPD

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*Corresponding author; email: wangyj@mail.hzau.edu.cn