Sains Malaysiana 41(2)(2012): 199–204

Comparison Between Pork and Wild Boar Meat (Sus scrofa ) by Polymerase

Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)

Perbandingan di antara Daging Babi dan Babi Hutan (Sus scrofa) dengan Menggunakan Analisis Tindak Balas Rantaian Polymerase-Polimorfisme Panjang Cebisan Pemotongan (PCR-RFLP)

Sahilah Abd. Mutalib*

School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E. Malaysia

Wan Sakeenah Wan Nazri, Safiyyah Shahimi, Norhayati Yaakob, Norrakiah Abdullah Sani,

Aminah Abdullah, Abdul Salam Babji & Maaruf Abd. Ghani

School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E. Malaysia

Received: 28 January 2011 /Accepted: 2 August 2011

ABSTRACT

A method of PCR-restriction fragment length polymorphism (RFLP) has been utilized to differentiate the mitochondrial genes of pork and wild boar meat (Sus scrofa). The amplification PCR products of 359 bp and 531 bp were successfully amplified from the cyt b gene of these two meats. The amplification product of pork and wild boar using mt-12S rRNA gene successfully produced a single band with molecular size of 456 bp. Three restriction endonucleases (AluI, HindIII and BsaJI) were used to restrict the amplification products of the mitochondrial genes. The restriction enzymes of AluI and BsaJI were identified as potential restriction endonucleases to differentiate those meats. HindIII enzyme was unable to restrict the PCR product of both meats. The genetic differences within the cyt b gene among the two meats were successfully confirmed by PCR-RFLP analysis.

Keywords: Polymerase chain reaction-restriction fragment length polymorphism; pork; wild boar meat (Sus scrofa)

ABSTRAK

Kaedah tindak balas rantaian polymerase-polimorfisme panjang cebisan pemotongan (PCR-RFLP) telah digunakan untuk membezakan gen mitokondria daging babi dan babi hutan (Sus scrofa). Hasil amplifikasi PCR, 359 pb dan 531 pb telah berjaya diamplikasikan daripada gen cyt b oleh kedua-dua daging. Sementara itu, hasil amplifikasi daging babi dan babi hutan menggunakan gen mt-12S rRNA telah berjaya menghasilkan jalur tunggal dengan saiz molekul 456 pb. Tiga enzim endonuklease pemotongan (AluI, HindIII danBsaJI) telah digunakan untuk memotong hasil amplifikasi gen mitokondria. Enzim pemotongan AluI dan BsaJI telah dikenalpasti sebagai endonuklease pemotongan yang berpotansi untuk membezakan kedua-dua daging. Enzim HindIII tidak boleh melakukan pemotongan terhadap hasil PCR kedua-dua daging. Perbezaan genetik di antara gen cyt b antara dua daging telah berjaya disahkan dengan analisis PCR-RFLP.

Kata kunci: Babi hutan (Sus scrofa); khinzir; tindak balas rantaian polimerase-polimorfisme panjang cebisan pemotongan

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*Corresponding author; email: sahilah@ukm.my