Sains Malaysiana 42(10)(2013): 1511–1516

 

Anti-inflammatory Effects of Labisia pumila (Blume) F. Vill-Naves. Aqueous Extract

(Kesan Ekstrak Larutan Labisia pumila (Blume) F. Vill-Naves. Sebagai Anti-radang)

 

R.A.M. Sanusi1, N.A. Ab Shukor2* & M.R.  Sulaiman3

1Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

 

2Institute of Tropical Forest and Forest Products (INTROP), Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

 

3Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

 

Received: 13 April 2013/Accepted: 12 May 2013

 

ABSTRACT

This study was carried out to evaluate the anti-inflammatory effects of three concentrations of Labisia pumila (Blume) F. Vill-Naves aqueous leaf extract in rats. The effects of these extracts as anti-inflammatory agents were determined using two experiments namely formalin-induced paw licking and carrageenan-induced paw oedema test. The exposure of inflammation to various treatments resulted in significant differences between treatments in formalin-induced paw licking in rats experiment whereas in phase 2, 50 mg kg-1 of L. pumila extract showed the most significant inhibition of 82.12%, followed by 10 mg kg-1 with 76.00% and 25 mg kg-1 with 57.80%. Similarly, different treatments showed significant effects at p<0.05 in the carrageenan inducing paw oedema experiment. All treatments were able to suppress the oedema formation induced by carrageenan as compared with the control. It is evident that the anti-inflammtory effect of every concentration of  L. pumila extract started as early as the first hour of carrageenan injection and showed the maximum inhibition during the fifth hour. Again, 50 mg kg-1 of L. pumila extract was found to be the best treatment that could reduce inflammation with highest inhibition of 64.59% followed by 25 mg kg-1 with 56.99% and 10 mg kg-1 with 5.55%. The result of this study has shown that these extracts of  L. pumila can be effective for anti-inflammation purposes which supports and justifies traditional uses of this plant.

 

Keywords: Anti-inflammation; carrageenan induce paw oedema test; formalin-induced paw licking test; Labisia pumila; medicinal plants

 

ABSTRAK

Kajian ini telah dijalankan untuk menilai kesan tiga kepekatan ekstrak larutan Labisia pumila sebagai anti-radang ke atas tikus. Kesan ekstrak sebagai anti-radang dikenal pasti melalui uji kaji formalin yang menyebabkan tikus menjilat tapak kaki dan karaginan yang menyebabkan kebengkakan pada tapak kaki. Kesan rawatan terhadap keradangan menunjukkan perbezaan bererti dalam pengurangan radang di dalam uji kaji formalin dan di dalam fasa 2, 50 mg kg-1 ekstrak L. pumila menunjukkan kesan pengurangan paling bererti sebanyak 82.12%, diikuti 10 mg kg-1 dengan 76.00% dan 25 mg kg-1 dengan 57.80%. Rawatan yang berbeza juga memberikan kesan yang bererti pada p<0.05 di dalam uji kaji karaginan yang menyebabkan kebengkakan tapak kaki. Semua rawatan yang diberikan dapat mengurangkan kebengkakan yang disebabkan oleh karaginan jika dibandingkan dengan kawalan. Adalah terbukti bahawa kesan anti-radang dalam setiap kepekatan ekstrak L. pumila bermula seawal jam pertama dan memberi kesan maksimum dalam pengurangan radang semasa atau pada jam kelima selepas suntikan karaginan. Sekali lagi kepekatan 50 mg kg-1 merupakan rawatan terbaik untuk mengurangkan radang dengan peratusan tertinggi sebanyak 64.59% diikuti dengan 25 mg kg-1 sebanyak 56.99% dan 10 mg kg-1 sebanyak 5.55%. Keputusan kajian menunjukkan bahawa ekstrak L. pumila adalah berkesan untuk digunakan sebagai rawatan anti-radang dan ini menyokong penggunaan tradisi tumbuhan ini.

 

Kata kunci: Anti-radang; Labisia pumila; tumbuhan ubatan; ujian jilat tapak kaki rangsangan formalin; ujian kebengkakan tapak kaki rangsangan karaginan

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*Corresponding author; email: anishukor@yahoo.com

 

 

 

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