Sains Malaysiana 42(1)(2013): 99–105

 

Lactate Dehydrogenase Activity During Tooth Movement under 1.0 N

and 1.5 N Continuous Force Applications

(Aktiviti Laktat Dehidrogenase Semasa Pergerakan Gigi dengan Aplikasi

Tekanan 1.0 N dan 1.5 N Secara Berterusan)

 

Shahrul Hisham Zainal Ariffin & Nurfathiha Abu Kasim

School of Bioscience and Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E. Malaysia

 

Rohaya Megat Abdul Wahab*

Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia

50300 Kuala Lumpur, Malaysia

 

Abdul Aziz Jemain

DELTA, School of Mathematics, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E.Malaysia

 

Received: 19 December 2011 / Accepted: 10 July 2012

 

ABSTRACT

 

The aim of this study was to observe the pattern of lactate dehydrogenase (LDH) activity in GCF and the rate of tooth movement at two different orthodontic forces (1.0 N and 1.5 N). Twelve subjects participated in this study and was chosen based on the inclusion criteria. Each subject received forces of 1.0 N and 1.5 N for tooth movement either on the left or right side of the maxillary canine. GCF sample was collected at mesial and distal sites of the canines before applying the appliance (week 0) and every week for 5 weeks after tooth movement (week 1 to week 5) where baseline activity served as control. LDH activity was assayed spectrophotometically at 340 nm. The tooth movements were measured from casted study models. LDH specific activity at mesial sites in 1.0 N and 1.5 N force groups, respectively increased significantly (p<0.05) only on week four and throughout the treatment when compared with baseline. At distal sites, LDH specific activity with 1.5 N was higher than 1.0 N throughout the five weeks of tooth movement. LDH specific activity with 1.5 N force increased at both mesial (week 2) and distal sites (week 3) with significant different (p<0.05) when compared with 1.0 N force. Tooth movement with 1.5 N showed significantly faster (p<0.05) at the end of week 5 when compared with 1.0 N. LDH has the potential as a biological marker of inflammation during tooth movement.A force of 1 N was more suitable to be used although less tooth movement was produced because less inflammation caused by the force can be useful in orthodontic treatment for patients with stabilised periodontal diseases compared with 1.5 N force.

 

Keywords: Biological marker; inflammation; lactate dehydrogenase; orthodontic force; tooth movement

 

ABSTRAK

Kajian ini bertujuan untuk melihat corak aktiviti laktat dehidrogenase (LDH) di dalam GCF dan kadar pergerakan gigi pada dua daya tekanan ortodontik yang berbeza (1.0 N dan 1.5 N). Dua belas orang subjek telah mengambil bahagian dalam kajian ini dan mereka dipilih berdasarkan beberapa kriteria yang telah ditetapkan. Setiap subjek menerima 1.0 N dan 1.5 N daya tekanan untuk pergerakan gigi sama ada pada bahagian kanan atau kiri gigi taring maksila. Sampel GCF dikumpul dari bahagian mesial dan distal gigi taring sebelum dipakaikan pendakap gigi (minggu 0) dan setiap minggu untuk lima minggu selepas gigi digerakkan (minggu 1 hingga minggu 5) dengan aktiviti basal dijadikan sebagai kawalan. Aktiviti LDH diasai menggunakan pendekatan spektrofotometri pada 340 nm. Pergerakan gigi diukur daripada model-model kajian yang telah dibentuk. Aktiviti spesifik LDH pada bahagian mesial dalam kumpulan tekanan 1.0 N dan 1.5 N masing-masing meningkat secara signifikan (p<0.05) hanya pada minggu 4 dan sepanjang rawatan berbanding kawalan. Pada bahagian distal, aktiviti spesifik LDH dengan 1.5 N adalah lebih tinggi berbanding 1.0 N sepanjang lima minggu pergerakan gigi. Aktiviti spesifik LDH dengan tekanan 1.5 N meningkat (p<0.05) pada kedua-dua bahagian mesial (minggu 2) dan distal (minggu 3) berbanding tekanan 1.0 N. Pergerakan gigi dengan 1.5 N lebih pantas (p<0.05) pada akhir minggu 5 berbanding dengan 1.0 N. LDH berpotensi sebagai penanda biologi untuk inflamasi semasa pergerakan gigi. Daya tekanan 1.0 N berbanding 1.5 N lebih sesuai digunakan walaupun ia menghasilkan kurang pergerakan gigi kerana penghasilan inflamasi yang rendah adalah penting dalam rawatan ortodontik kepada pesakit periodontal yang telah stabil.

 

Kata kunci: Inflamasi; laktat dehidrogenase; penanda biologi; pergerakan gigi; tekanan ortodontik

REFERENCES

 

Apajalahti, S., Sorsa, T., Railavo, S. & Ingman, T. 2003. The in vivo levels of matrix metalloproteinase -1 and -8 in gingival crevicular fluid during initial orthodontic tooth movement. J. Dent. Res. 82: 1018-1022.

Asma, A.A.A., Rohaya, M.A.W. & Shahrul Hisham, Z.A. 2011. Pattern of crevicular alkaline phosphatase during orthodontic tooth movement: Leveling & alignment stage. Sains Malaysiana 40(10): 1147-1151.

Drent, M., Cobben, N.A.M., Henderson, R.F., Wouters, E.F.M. & van Dieijen-Visser, M. 1996. Usefulness of lactate dehydrogenase and its isoenzymes as indicators of lung damage or inflammation. Eur. Resp. J. 9: 1736-1742.

Jin, L. 2007. Periodontic-orthodontic interactions-rationale, sequence and clinical implications. Hong Kong Dent. J. 4: 60-64.

Kavadia-Tsatala, S., Kaklamanos, E.G. & Tsalikis, L. 2002. Effects of orthodontic treatment on gingival crevicular fluid flow rate and composition: Clinical implications and applications. Int. J. Adult Orthod. Orthognath. Surg. 17: 191-205.

King, G.J., Keeling, S.D. & Wronski, T.J. 1991. Histomorphometric study of alveolar bone turnover in orthodontic tooth movement. Bone 12: 401-9.

Krishnan, V. & Davidovitch, Z. 2006. Cellular, molecular and tissue-level reactions to orthodontic force. Am. J. Orthod. Dentofacial Orthop. 129(469): e1-32.

Lamster, I.B. & Ahlo, J.K. 2007. Analysis of gingival crevicular fluid as applied to the diagnosis of oral and systemic diseases. Ann. N. Y. Acad. Sci. 1098: 216-229.

Perinetti, G., Baccetti, T., Contardo, L. & Di Lenarda, R. 2011. Gingival crevicular fluid alkaline phosphatase activity as a non-invasive biomarker of skeletal maturation. Orthod. Craniofac. Res. 14: 44-50.

Perinetti, G., Paolantonio, M., D’Attilio, M., D’Archivio, D., Dolci, M., Femminella, Beatrice., Festa, F. & Spoto, G. 2003. Aspartate aminotransferase activity in gingival crevicular fluid during human orthodontic tooth movement. A controlled short-term longitudinal study. J. Periodontol. 74: 145-152.

Perinetti, G., Paolantonio, M., D’Attilio, M., D’Archivio, D., Tripodi, D., Femminella, B., Festa, F. & Spoto, G. 2002. Alkaline phosphatase activity in gingival crevicular fluid during human orthodontic tooth movement. Am. J. Orthod. Dentofacial. Orthop. 122: 548-556.

Perinetti, G., Serra, E., Paolantonio, M., Bruè, C., Di Meo, S., Filippi, M.R., Festa, F. & Spoto, G. 2005. Lactate dehydrogenase activity in human gingival crevicular fluid during orthodontic treatment: A controlled, short-term longitudinal study. J. Periodontol. 76: 411-417.

Py, O-M., Kurol, J. & Lundgren, D. 1996. The effects of a four-fold increased orthodontic force magnitude on tooth movement and root resorptions. An intra-individual study in adolescents. Eur. J. Orthod. 18: 287-294.

Ren, Y., Jaap, C.M. & Kuijpers-Jagtman, A.M. 2003. Optimum force magnitude for orthodontic tooth movement: A systematic literature review. Angle Orthod. 73: 86-92.

Roberts-Harry, D. & Sandy, J. 2004. Orthodontics. Part 11: Orthodontic tooth movement. Bri. Dent. J. 196: 391-394.

Rohaya, M.A.W., Maryati, M.D., Sahidan, S., Asma Alhusna, A.A., Abdul Aziz, J., Nurfathiha, A.K., Zulham, Y. & Shahrul Hisham, Z.A. 2011. Crevicular tartrate resistant acid phosphatase activity and rate of tooth movement under different continuous force applications. Afr. J. Pharm. Pharmacol. 5(20): 2213-2219.

Rohaya, M.A.W., Shahrul Hisham, Z.A., Khazlina, K. 2008. The activity of aspartate aminotransferase during canine retraction (bodily tooth movement) in orthodontic treatment. J. Med. Sci. 8(6): 553-558.

Rygh, P. 1972. Ultrastructural changes in pressure zones of rat molar periodontium incident to orthodontic movement. Acta Odontol. Scand. 30: 575-593.

Rygh, P. 1976. Ultrastructural changes in tension zones of rat molar periodontium incident to orthodontic movement. Am. J. Orthod. 70: 269-281.

Sarah, A.A. & Sukumaran, A. 2011. Lactate dehydrogenase activity in gingival crevicular fluid as a marker in orthodontic tooth movement. Open Dent. J. 5: 105-109.

Serra, E., Perinetti, G., D’Attilio, M., Cordella, C., Paolantonio, M. & Spoto, G. 2003. Lactate dehydrogenase activity in gingival crevicular fluid during orthodontic treatment. Am. J. Orthod. Dentofacial Orthop. 124: 206-211.

Shahrul Hisham, Z.A., Mohd Faiz, E., Rohaya, M.A.W., Yosni, B. & Sahidan, S. 2010. Profile of lactate dehydrogenase, tartrate resistant acid phosphatase and alkaline phosphatase in saliva during orthodontic tooth movement. Sains Malaysiana 39(3): 405-412.

Toms, S.R., Lemons, J.E., Bartolucci, A.A. & Eberhardt, A.W. 2002. Nonlinear stress-strain behavior of periodontal ligament under orthodontic loading. Am. J. Orthod. Dentofacial Orthop. 122: 174-179.

Víctor Alonso De La Peña, Pedro Diz Dios & Rafael Tojo Sierra. 2007. Relationship between lactate dehydrogenase activity in saliva and oral health status. Arch. Oral. Biol. 52(10): 911-915.

Wise, G.E. & King, G.J. 2008. Mechanisms of tooth eruption and orthodontic tooth movement. J. Dent. Res. 7(5): 414-434.

 

 

*Corresponding author; email: shahroy7@gmail.com

 

 

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