Sains Malaysiana 42(5)(2013): 629–633

 

Characterization of Fusarium semitectum Isolates from Vegetable Fruits

(Pencirian Pencilan Fusarium semitectum daripada Buahan Sayuran)

 

 

Z. Latiffah*, M.S. Nurul Huda & T.M.A. Tengku Ahmad Akram

School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia

 

 

Received: 1 December 2011/Accepted: 14 November 2012

 

ABSTRACT

Fusarium semitectum is a widespread species occurring in various types of substrate. Fifteen F. semitectum isolates were recovered from several types of vegetable fruits showing fruit rot symptoms, namely long bean (Vigna sesquilpedalis), okra (Abelmoschus esculentus), loofa (Luffa acutangula), bitter gourd (Momordica charantia), cucumber (Cucumis sativus) and green chilli (Capsicum annum). The identification of the F. semitectum isolates was based on morphological characteristics of macroconidia and microconidia, presence of mesoconidia and colony pigmentation. The isolates were then characterized using vegetative compatibility group (VCG) and sequencing of translation elongation factor-1α gene (TEF-1α). From VCG analysis, 11 isolates were assigned to six VCGs and another four isolates were self-incompatible. The results of the phylogenetic analysis using TEF-1α sequences showed that the isolates were phyogenetically distinct although the morphological characteristics were very similar and generally the grouping of the isolates was not according to the host. Both VCG and phylogenetic analyses indicated that F. semitectum isolates from vegetable fruits were highly variable which suggested that the isolates belong to a species complex.

 

Keywords: Fusarium semitectum; TEF-1α; VCG; vegetable fruits

 

ABSTRAK

Fusarium semitectum merupakan spesies yang tersebar dengan meluas, menduduki pelbagai jenis substrat. Lima belas pencilan F. semitectum telah diperoleh daripada beberapa jenis buahan sayuran yang menunjukkan gejala reput buah iaitu kacang panjang (Vigna sesquilpedalis), okra (Abelmoschus esculentus), petola (Luffa acutangula), peria (Momordica charantia), timun (Cucumis sativus) dan cili hijau(Capsicum annum). Pencilan F. semitectum telah dikenal pasti berdasarkan ciri-ciri morfologi makrokonidia, mikrokonidia, kehadiran mesokonidia and pigmentasi koloni. Pencilan tersebut kemudiannya dicirikan menggunakan kumpulan keserasian vegetatif (VCG) dan penjujukan gen faktor pemanjangan translasi (TEF-1α). Daripada analisis VCG, 11 pencilan diletakkan ke dalam enam kumpulan VCG dan empat pencilan yang selebihnya menunjukkan ketidakserasian sendiri. Keputusan analisis filogenetik menggunakan jujukan TEF-1α menunjukkan pencilan tersebut berbeza secara filogenetik walaupun ciri-ciri morfologinya adalah serupa dan secara amnya pencilan tersebut tidak dikumpulkan mengikut perumah. Kedua-dua analisis VCG dan filogenetik menunjukkan pencilan F. semitectum daripada buahan sayuran mempunyai variasi yang tinggi yang dicadangkan pencilan tersebut tergolong dalam satu kompleks spesies.

 

Kata kunci: Buahan sayuran; Fusarium semitectum; TEF-1α; VCG

REFERENCES

Burgess, L.W., Liddell, C.M. & Summerell, B.A. 1988. Laboratory Manual for Fusarium Research. Sydney: University of Sydney.

Booth, C. 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, United Kingdom.

Carter W.W. 1979. Corky dry rot of cantaloupe caused by F. roseum ‘semitectum’. Plant Disease Reporter 63: 1080-1084.

Correll, J.C. 1991. The relationship between formae speciales, races and vegetative compatibility groups in Fusarium oxysporum. Phytopathology 81: 1061-1064.

Dhingra, O.D. & Muchovej, J.J. 1979. Pod rot, seed rot and root rot of snap bean and dry bean caused by F. semitectum. Plant Disease Reporter 63: 84-87.

Geiser, D.M., Jimenz Gasco, M.M., Kang, S., Mkalowska, I., Veeraraghavan, N., Ward, T.J., Zhang, N., Kuldau, G.A. & O’Donnell, K. 2004. Fusarium-ID v. 1.0: ADNAsequence database for identifying Fusarium. European Journal of Plant Pathology 110: 473-479.

Griffee, P.J. 1976. Pathogenicity of some fungi isolate from diseased crowns of banana hands. Phytopathilogische Zeitschrift 85: 206-216.

Griffee, P.J. & Burden, O.J. 1976. Fungi associated with crown rot of boxed bananas in the Windward Islands. Phytopathogische Zeitschrift 85: 149-158.

Kerenyi, Z., Taborhegyi, E., Pomazi, A. & Hornok, L. 1997. Variability amongst strains of Fusarium poae assessed by vegetative compatibility and RAPD polymorphism. Plant Pathology 46: 882-889.

Latiffah, Z., Mohd Zariman, M. & Baharuddin, S. 2007. Diversity of Fusarium species in cultivated soils in Penang. Malaysian Journal of Microbiology 3(1): 27-30.

Latiffah, Z., Padzilah, M.I., Baharuddin, S. & Maziah, Z. 2009. Fusarium species in forest soil of Bird Valley. Malaysian Journal of Microbiology 5(2): 132-133.

Latiffah, Z. & Nur Hidayah, A.R. 2011. Endophytic Fusarium spp. from wild banana (Musa acuminata) roots. African Journal of Microbiology Research 5(21): 3600-3602.

Leslie, J.F. 1990. Genetic exchange within sexual and asexual populations of the genus Fusarium. In Fusarium wilt of Banana, edited by Ploetz, R.C. American Phytopathological Society, St. Paul, MN.

Leslie, J.F. 1993. Fungal vegetative compatibility. Annual Review of Phytopathology 31: 127-150.

Leslie, J.F. & Summerell, B.A. 2006. The Fusarium Laboratory Manual. USA: Blackwell Publishing, Ames, IA.

McDonald, D. 1964. Annual Report for the Institute of Agricultural Research Samaru for the year 1962-1963.

Masratul Hawa, M., Salleh, B. & Latiffah, Z. 2010. Characterization and intraspecific variation of Fusarium semitectum (Berkeley & Ravenel) associated with red-fleshed dragon fruit (Hylocereus polyrhizus [Weber] Britton & Rose) in Malaysia. African Journal of Biotechnology 9(3): 273-284.

O’Donnell, K., Kistler, H.C., Cigellink, E. & Ploetz, R.C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Science USA 95: 2044-2049.

Puhalla, J.E. & Spieth, P.T. 1985. Acomparison of heterokaryosis and vegetative incompatibility among varieties of Gibberella fujikuroi (Fusarium moniliforme). Experimental Mycology 9: 39-47.

Satyaprasad, K., Bateman, G.L. & Ward, E. 2000. Comparisons of isolates of Fusarium avenaceum from white lupin and other crops by pathogenicity tests, DNAanalyses and vegetative compatibility tests. Journal of Phytopathology 148: 211-219.

Seta, S., Gonzalez, M. & Lori, G. 2004. First report of walnut canker caused by Fusarium incarnatum in Argentina. Plant Pathology 53: 248.

Smith-White, J.L., Gunn, L.V. & Summerell, B.A. 2001. Analysis of diversity within Fusarium oxysporum populations using molecular analysis and vegetative compatibility grouping. Australasian Plant Pathology 30: 153-157.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731-2739.

Zaccardelli, M., Balmas, V., Altomare, C., Corazza, L. & Scotti, C. 2006. Characterization of Italian isolates of Fusarium semitectum from alfalfa (Medicago sativa) by AFLP analysis, morphology, pathogenicity and toxin production. Journal of Phytopathology 154: 454-460.

 

 

*Corresponding author; email: Lfah@usm.my

 

 

 

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