Sains Malaysiana 42(5)(2013): 635–642

 

Effects of GnRHa on Plasma Sex Steroid Hormones of River Catfish Hemibagrus nemurus (Valenciennes 1840)

(Kesan GnRHa Terhadap Hormon Seks Steroid Plasma Ikan Baung Hemibagrus nemurus (Valenciennes 1840))

 

Fatimat Adenike Adebiyi, Siti Shapor Siraj*, Sharr Azni Harmin & Annie Christianus

Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia

43400 UPM Serdang, Selangor, Malaysia

 

Received: 12 March 2012/Accepted: 4 October 2012

 

ABSTRACT

The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 μg/kg, 20 μg/kg and 50 μg/kg body weight (BW) of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 μg/kg. Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 μg/kg and 50 μg/kg BW GnRHa treatments and a dose of 5 μg/kg BW GnRHa produced a slow response to steroidogenesis. Treatment with 50 μg/kg BW GnRHa produced the best result. The highest level of testosterone, 11-ketotestosterone and 17β-estradiol were observed at 24 h for all treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus. Therefore, it can be used as an inducing agent for the control of reproduction in H. nemurus.

 

Keywords: Gonadotropin releasing hormone analogue (GnRHa); Hemibagrus nemurus; testosterone; 11-ketotestosterone; 17β-estradiol

ABSTRAK

Kesan analog hormon lepasan gonadotropin (GnRHa) terhadap hormon seks steroid plasma ikan baung Hemibagrus nemurus telah dikaji. Enzim berkaitan imunosorben asei (ELISA) telah digunakan untuk mengukur hormon. Ikan telah dirawat dengan salina (kawalan) dan GnRHa pada pelbagai dos 5 μg/kg, 20 μg/kg dan 50 μg/kg berat badan (BW) ikan. Sampel darah telah dikumpul pada 0, 6, 12 dan 24 jam selepas suntikan hormon. Keputusan menunjukkan bahawa GnRHa meningkatkan hormon seks steroid plasma walaupun pada dos yang rendah, 5 μg/kg. Peningkatan yang signifikan (p< 0.05) dalam tahap seks steroid plasma telah diperhatikan pada rawatan 20 μg/kg dan 50 μg/kg BW GnRHa sementara dos 5 μg/kg BW GnRHa menghasilkan tindak balas yang perlahan untuk steroidogenesis. Rawatan GnRHa pada dos 50 μg/kg BW menunjukkan hasil yang terbaik. Tahap tertinggi testosteron, 11-ketotestosterone dan-estradiol 17β telah diperhatikan pada 24 jam untuk semua rawatan. Keputusan menunjukkan bahawa GnRHa meningkatkan pengeluaran steroid dalam plasma H. nemurus. Oleh itu, ia boleh digunakan sebagai ejen pendorong untuk kawalan pembiakan H. nemurus.

 

Kata kunci: Analog hormon lepasan gonadotropin (GnRHa); Hemibagrus nemurus; testosteron; 11-ketotestosterone; 17β-estradiol

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*Corresponding author; email: shapor@putra.upm.edu.my