Sains Malaysiana 42(7)(2013): 921–926

 

Peninsular Malaysia’s Negrito Orang Asli and Its Theory of African Origin

(Orang Asli Negrito di Semenanjung Malaysia dan Teori Asal Usul Afrika)

 

Endom Ismail*, Farahnaz Amini, Shairah Abdul Razak & Husna Mohd Zaini

School of Bioscience and Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia

 

Bin Alwi Zilfalil

School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian,

Kelantan, Malaysia

 

Reza Farhour

Saint James School of Medicine, Albert Lake Drive, The Quarter, A-1 2640,Anguilla

 

Received: 24 August 2012/Accepted: 4 December 2012

 

 

ABSTRACT

 

Negritos of Peninsular Malaysia have physical features which strongly resemble the African pygmies rather than any of the other main South East Asian ethnic groups. In addition, their features are also completely different from the two other large sub-groups of the Peninsular Malaysia Orang Asli, i.e. Senoi and Proto-Malay. In this study, we genetically screened three African-specific markers, Glucose-6 Phosphate Dehydrogenase (G6PD) gene PvuII Type 2 polymorphism and A- mutation; and Sickle Cell trait in 103 unrelated individuals with G6PD deficiency. None of the Negritos’ samples carried A- and Sickle cell mutations but all males and females have the PvuII Type 2 polymorphism. The same results were seen in all DNA samples of the Malaysian’s Malay, Chinese and Indians. Additionally, all females in this study were homozygous for PvuII Type 2 polymorphism. Thus, we concluded that this polymorphism is widespread in all Malaysian population and is not unique to just Africans. However, these findings indicated that the polymorphism was widely conserved and can be used to study the African descendant in any world population hitherto supporting the ‘Out of Africa’ theory.

 

Keywords: G6PD; Malaysia; Negrito; Orang Asli

 

ABSTRAK

 

Negrito di Semenanjung Malaysia mempunyai ciri-ciri fizikal yang sangat menyerupai orang Pigmi di Afrika berbanding kumpulan etnik lain di Asia Tenggara. Sebagai tambahan, ciri-ciri mereka juga secara keseluruhannya berbeza berbanding dua lagi sub-kumpulan Orang Asli di Semenanjung Malaysia iaitu Senoi dan Melayu-Proto. Dalam kajian ini, kami membuat penyaringan genetik menggunakan tiga penanda khas populasi Afrika, polimorfisme PvuII Type 2 dan mutasi A- untuk G6PD gen serta mutasi sel sabit ke atas 103 individu tak bersaudara yang mengalami kekurangan G6PD. Kesemua sampel Negrito tidak membawa mutasi A- dan sel sabit tetapi kesemua lelaki dan perempuan dalam kajian ini mempunyai polimorfisme PvuII Type 2. Hasil yang sama dicerap dalam semua sampel DNA Melayu, Cina dan India di Malaysia. Tambahan lagi, semua perempuan dalam kajian ini homozigus bagi polimorfisme PvuII Type 2. Oleh itu, kami menyimpulkan bahawa polimorfisma ini tersebar luas dalam kesemua populasi Malaysia dan tidak khusus untuk orang Afrika sahaja. Walau bagaimanapun, penemuan kajian ini memberi petunjuk bahawa polimorfisme ini terpelihara secara meluas dan boleh digunakan untuk mengkaji keturunan Afrika bagi mana-mana populasi dunia, seterusnya menyokong teori ‘Out of Africa’.

 

Kata kunci: G6PD; Malaysia; Negrito; Orang Asli

REFERENCES

 

Abdulla, M.A., Ahmed, I., Assawamakin, A., Bhak, J., Brahmachari, S.K., Calacal, G.C., Chaurasia, A., Chen, C.H., Chen, J., Chen, Y.T., Chu, J., Cutiongco-de la Paz, E.V.M.C., De Ungria, M.C.A., Delfin, F.C., Edo, J., Fuchareon, S., Ghang, H., Gojobori, T., Han, J., Ho, S.F., Hoh, B.P., Huang, W., Inoko, H., Jha, P., Jinam, T.A., Jin, L., Jung, J., Kangwanpong, D., Kampuansai, J.C., Kennedy, G.C., Khurana, P., Kim, H.L., Kim, K., Kim, S., Kim, W.Y., Kimm, K., Kimura, R., Koike, T., Kulawonganunchai, S., Kumar, V., Lai, P.S., Lee, J.Y., Lee, S., Liu, E.T., Majumder, P.P., Kumar, M., Marzuki, S., Mitchell, W., Mukerji, M., Naritomi, K., Ngamphiw, C., Niikawa, N., Nishida, N., Oh, B., Oh, S., Ohashi, J., Oka, A., Ong, R., Padilla, C.D., Palittapongarnpim, P., Perdigon, H.B., Phipps, M.E., Png, E., Sakaki, Y., Salvador, J.M., Sandraling, Y., Scaria, V., Seielstad, M., Sidek, M.R., Sinha, A., Srikummool, M., Sudoyo, H., Sugano, S., Suryadi, H., Suzuki, Y., Tabbada, K.A., Tan, A., Tokunaga, K., Tongsima, S., Villamor, L.P., Wang, E., Ying, W.Y., Wang, H., Wu, J.Y., Xiao, H., Xu, S., Yang, J.O., Shugart, Y.Y., Yoo, H.S., Yuan, W., Zhao, G. & Zilfalil, A. 2009. Mapping human genetic diversity in Asia. Science 326: 1541-1545.

Ainoon, O., Boo, N.Y., Yu, Y.H., Cheong, S.K., Hamidah, H.N. & Lim, J.H. 2004. Complete molecular characterisation of glucose-6-phosphate dehydrogenase (G6PD) deficiency in a group of Malaysian Chinese neonates. Malays. J. Pathology 26(2): 89-98.

Ainoon, O., Yu, Y.H., Amir Muhriz, A.L., Boo, N.Y., Cheong, S.K. & Hamidah, N.H. 2003. Glucose-6-phosphate dehydrogenase (G6PD) variants in Malaysian Malays. Human Mutation 21(1): 101.

Ainoon, O., Yu, Y.H., Amir Muhriz, A.L., Boo, N.Y., Cheong, S.K. & Hamidah, N.H. 2002. Glucose-6-phosphate dehydrogenase (G6PD) variants in Malaysian Malays. Human Mutation 21: 101-109.

Ainoon, O., Joyce, J., Boo, N.Y., Cheong, S.K., Zainal, Z.A. & Hamidah, N.H. 1999. Glucose-6-phosphate dehydrogenase (G6PD) variants in Malaysian Chinese. Human Mutation 14(4): 352.

Amini, F., Ismail, E. & Zilfalil, B.A. 2011. Prevalence and molecular study of G6PD deficiency in Malaysian Orang Asli. Internal Medicine J. 41(4): 351-353.

Balgir, R.S. 2010. Genetic diversity of hemoglobinopathies, G6PD deficiency, and ABO and Rhesus blood groups in two isolates of a primitive Kharia Tribe in Sundargarh District of Northwestern Orissa, India. Community Genetics 1: 117-123.

Beutler, E. & Kuhl, W. 1990. Linkage between a PvuII restriction fragment length polymorphism and G6PD A-202A/376G: Evidence for a single origin of the common G6PD A- mutation. Human Genetics 85(1): 9-11.

Beutler, E. & Vulliamy, T.J. 2002. Hematologically important mutations: Glucose-6-phosphate dehydrogenase. Blood Cells Molecular 28(2): 93-103.

Beutler, E., Kuhl, W., Ramirez, E. & Lisker, R. 1991. Some Mexican glucose-6-phosphate dehydrogenase variants revisited. Human Genetics 86(4): 371-374.

Chockkalingam, K. & Board, P.G. 1980. Further evidence for heterogeneity of glucose-6-phosphate dehydrogenase deficiency in Papua New Guinea. Human Genetics 56: 209-212.

Coetzee, M.J., Bartleet, S.C., Ramsay, M. & Jenkins, T. 1992. Glucose-6-phosphate dehydrogenase (G6PD) electrophoretic variants and the PvuII polymorphism in southern African populations. Human Genetics 89(1): 111-113.

Denic, S. & Nicholls, M.G. 2007. Genetic benefits of consanguinity through selection of genotypes protective against malaria. Human Biology 79(2): 145-158.

Fey, M.F., Wainscoat, J.S., Mukwala, E.C., Falusi, A.G., Vulliamy, T.J. & Luzzatto, L. 1990. A PvuII restriction fragment length polymorphism of the glucose-6-phosphate dehydrogenase gene is an African-specific marker. Human Genetics 84(5): 471-472.

Hue, N.T., Charlieu, J.P., Chau, T.T., Day, N., Farrar, J.J., Hien, T.T. & Dunstan, S.J. 2009. Glucose-6-phosphate dehydrogenase (G6PD) mutations and haemoglobinuria syndrome in the Vietnamese population. Malaria Journal 8: 152.

Iwai, K., Hirono, A., Matsuoka, H., Kawamoto, F., Horie, T., Lin, K., Tantular, I.S., Dachlan, Y.P., Notopuro, H., Hidayah, N.I., Salim, A.M., Fujii, H., Miwa, S. & Ishii, A. 2001. Distribution of glucose-6-phosphate dehydrogenase mutations in Southeast Asia. Human Genetics 108: 445-449.

Joseph, R., Ho, L.Y., Gomez, J.M., Rajdurai, V.S., Sivasankaran, S. & Yip, Y.Y. 1999. Mass newborn screening for glucose-6-phosphate dehydrogenase deficiency in Singapore. Southeast Asian Journal Tropical Medical Public Health 30(Suppl. 2): 70-71.

Kay, A.C., Kuhl, W., Prchal, J. & Beutler, E. 1992. The origin of glucose-6-phosphate-dehydrogenase (G6PD) polymorphisms in African-Americans. American J. Human Genetics 50(2): 394-398.

Louicharoen, C. & Nuchprayoon, I. 2005. G6PD Viangchan (871G>A) is the most common G6PD-deficient variant in the Cambodian population. Journal Human Genetics 50: 448-452.

Manco, L., Botigué, L.R., Ribeiro, M.L. & Abade, A. 2007. G6PD deficient alleles and haplotype analysis of human G6PD locus in São Tomé e Príncipe (West Africa). Human Biology 79(6): 679-686.

Matsuoka, H., Arai, M., Yoshida, S., Tantular, I.S., Pusarawati, S., Kerong, H. & Kawamoto, F. 2003. Five different glucose-6-phosphate dehydrogenase (G6PD) variants found among 11 G6PD-deficient persons in Flores Island, Indonesia. Journal Human Genetics 48: 541-544.

Matsuoka, H., Wang, J., Hirai, M., Arai, M., Yoshida, S., Kobayashi, T., Jalloh, A., Lin, K. & Kawamoto, F. 2004. Glucose-6-phosphate dehydrogenase (G6PD) mutations in Myanmar: G6PD Mahidol (487G>A) is the most common variant in the Myanmar population. Journal Human Genetics 49: 544-547.

Meilleur, K.G., Coulibaly, S., Traoré, M., Landouré, G., La Pean, A., Sangaré, M., Mochel, F., Traoré, S., Fischbeck, K.H. & Han, H.R. 2011. Genetic testing and counseling for hereditary neurological diseases in Mali. J. Community Genetics 2: 33-42.

Miller, S.A. & Dykes, D.D. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16(3): 1215.

Nuchprayoon, I., Louicharoen, C. & Charoenvej, W. 2008. Glucose-6-phosphate dehydrogenase mutations in Mon and Burmese of southern Myanmar. Journal Human Genetics 53(1): 48-54.

Nuchprayoon, I., Sanpavat, S. & Nuchprayoon, S. 2002. Glucose-6-phosphate dehydrogenase (G6PD) mutations in Thailand: G6PD Viangchan (871G>A) is the most common deficiency variant in the Thai population. Human Mutation 19: 185.

Padilla, C., Nishiyama, K., Shirakawa, T. & Matsuo, M. 2003. Screening for glucose-6-phosphate dehydrogenase deficiency using a modified formazan method: A pilot study on Filipino male newborns. Pediatic International 45: 10-15.

Poon, M.C., Hall, K., Scott, C.W. & Prchal, J.T. 1988. G6PD Viangchan: A new glucose 6-phosphate dehydrogenase variant from Laos. Human Genetics 78: 98-99.

Saad, S.T., Salles, T.S., Carvalho, M.H. & Costa, F.F. 1997. Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in Brazil. Human Heredity 47(1): 17-21.

Shi, H., Dong, Y., Wen, B., Xiao, C.J., Underhill, P.A., Shen, P., Chakraborty, R., Jin, L. & Su, B. 2005. Y-chromosome evidence of southern origin of the East Asian-specific haplogroup O3-M122. American J. Human Genetics 77: 408-419.

Tishkoff, S.A., Varkonyi, R., Cahinhinan, N., Abbes, S., Argyropoulos, G., Destro-Bisol, G., Drousiotou, A., Dangerfield, B., Lefranc, G., Loiselet, J., Piro, A., Stoneking, M., Tagarelli, A., Tagarelli, G., Touma, E.H., Williams, S.M. & Clark, A.G. 2001. Haplotype diversity and linkage disequilibrium at human G6PD: Recent origin of alleles that confer malarial resistance. Science 293(5529): 455-462.

 

 

*Corresponding author; email: eismail@ukm.my

 

 

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