Sains Malaysiana 43(5)(2014): 715–722

In vitro Regeneration and Acclimatization Protocols of Selected Ornamental Plants

(Agapanthus praecox, Justicia betonica and Celosia cristata)

(Protokol Regenerasi Secara In vitro dan Aklimatasi Tumbuhan Hiasan Terpilih

(Agapanthus praecox, Justicia betonica dan Celosia cristata))

JAMILAH SYAFAWATI YAACOB*^{1 2}, AZANI SALEH^{1 3}, HASHIMAH ELIAS¹, SAKINAH ABDULLAH¹, NORAINI MAHMAD¹& NORMADIHA MOHAMED¹

¹Institute of Biological Sciences, Faculty of Science, University of Malaya

50603 Kuala Lumpur, Malaysia

²Centre for Research in Biotechnology for Agriculture (CEBAR), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

³School of Applied Science, MARA University of Technology, 40450 Shah Alam, Selangor, Malaysia

Received: 20 May 2013/Accepted: 4 September 2013

ABSTRACT

This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. praecox and C. cristata, as well as explants from intact plants of J. betonica were utilized to achieve complete plant regeneration of these species. MS medium supplemented with various hormones, with an emphasis on BAP and NAA were tested to obtain direct and indirect regeneration. Both A. praecox (bulbs) and C. cristata (shoots) formed complete plantlets on MS added with 0.5 – 2.0 mg/l BAP and NAA, while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were attempted to acclimatize the regenerants, with A. praecox gave the highest percentage of survival rates (96.67%), followed by J. betonica (80.00%) and C. cristata (75.00%).

Keywords: Acclimatization; Agapanthus praecox; Celosia cristata; Justicia betonica; plant growth regulators

ABSTRAK

Kertas ini menerangkan keberkesanan hormon pertumbuhan BAP dan NAA terhadap regenerasi tumbuhan hiasan terpilih; Agapanthus praecox, Justicia betonica dan Celosia cristata. Pelbagai eksplan (daun, batang, hujung pucuk dan ubi) yang berasal daripada anak benih aseptik A. praecox dan C. cristata yang berusia satu bulan, serta eksplan daripada tumbuhan utuh J. betonica telah digunakan untuk menperoleh regenerasi lengkap spesies ini. Media MS yang dibekalkan dengan pelbagai hormon, dengan penekanan kepada BAP dan NAA telah diuji untuk mendapatkan regenerasi secara langsung atau tidak langsung. Kedua-dua A. praecox (ubi) dan C. cristata (pucuk) membentuk anak pokok lengkap di dalam media MS yang ditambah dengan 0.5 - 2.0 mg/L BAP dan NAA, manakala regenerasi secara langsung telah dicapai oleh J. betonica di dalam media MS yang mengandungi BAP. Beberapa kaedah telah dicuba dalam pengikliman anak pokok, dengan A. praecox memberikan peratusan kadar kelangsungan hidup tertinggi (96.67%), diikuti oleh J. betonica (80.00%) dan C. cristata (75.00%).

Kata kunci: Agapanthus praecox; Celosia cristata; hormon pertumbuhan; Justicia betonica; pengikliman

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*Corresponding author; email: jam_sya@yahoo.com