Sains Malaysiana 44(12)(2015): 1693–1699

 

Development of the Compact UV Illuminator with Single UV Lamp

(Pembangunan Iluminator UV Padat dengan Satu Lampu UV)

JONG-DAE KIM2,3, DEUK-JU LEE1,3, SANG-YOON KIM1,3, YU-SEOP KIM2,3,

HYE-JEONG SONG2,3 & CHAN-YOUNG PARK*2,3

 

Deuk-Ju Lee & Sang-Yoon Kim

1Department of Computer Engineering, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gang-won-do, 200-702, Korea

 

2Department of Ubiquitous Computing, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gang-won-do, 200-702, Korea

 

3Bio-IT Research Center, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 200-702,

Korea

 

Received: 1 September 2014/Accepted: 23 June 2015

 

ABSTRACT

This paper presents a low-cost method of constructing the compact UV illuminator, which is considered as an important component of a gel documentation system. The procedure involves using a smallest-possible UV lamp and a motor which moves the UV lamp in the UV illuminator instead of conventional 4 UV lamps. A comparative analysis of images produced by using the commercial gel documentation system and our prototype was carried out. These comparisons were done in real DNA gel as well as a reference plate made of quantum dot. The plate was composed of the chambers filled with various densities of the quantum dot instead of the Agarose gel containing the ETBR in order to increase the accuracy of comparison and the convenience of experiments. Despite the use of only 1 UV lamp, the proposed system demonstrated a similar imaging performance compared with the conventional gel documentation system equipped with 4 UV lamps, resulting in the great reduction of the system cost.

 

Keywords: DNA detection; gel documentation system; gel image analysis; quantum dot; UV illuminator

 

ABSTRAK

Kertas ini membentangkan kaedah kos rendah membina iluminator UV yang padat dan dianggap sebagai satu komponen yang penting dalam sistem dokumentasi gel. Prosedur ini melibatkan penggunaan lampu UV terkecil dan motor yang menggerakkan lampu UV dalam iluminator UV dan bukannya lampu konvensional 4 UV. Satu analisis perbandingan imej yang dihasilkan dengan menggunakan sistem dokumentasi perdagangan gel dan prototip kami telah dijalankan. Perbandingan ini telah dijalankan dalam gel DNA sebenar serta plat rujukan buatan daripada dot kuantum. Plat tersebut terdiri daripada ruang dalam yang dipenuhi dengan pelbagai kepadatan dot kuantum dan bukan gel Agarosa yang mengandungi ETBR untuk meningkatkan ketepatan perbandingan dan kemudahan uji kaji. Walaupun hanya menggunakan 1 lampu UV, sistem yang dicadangkan menunjukkan prestasi imej yang sama berbanding dengan sistem dokumentasi gel konvensional dilengkapi dengan 4 lampu UV, seterusnya mengurangkan sistem kos.

 

Kata kunci: Dot kuantum; gel analisis imej; pengesanan DNA; sistem dokumentasi gel; UV iluminator


 

REFERENCES

Bajla, I., Hollander, I., Fluch, S., Burg, K. & Kollar, M. 2005. An alternative method for electrophoretic gel image analysis in the GelMaster software. Computer Methods and Programs in Biomedicine 77(3): 209-231.

Goldmann, T., Zyzik, A., Loeschke, S., Lindsay, W. & Vollmer, E. 2001. Cost-effective gel documentation using a web-cam. Journal of Biochemical and Biophysical Method 50(1): 91-95.

Kodziusa, R., Xiao, K., Wu, J., Yi, X., Gong, X., Foulds, I.G. & Wen, W. 2012. Inhibitory effect of common microfluidic materials on PCR outcome. Sensors and Actuators B: Chemical 161(1): 349-358.

Lee, D.J., Kim, S.Y., Kim, J.D., Kim, Y.S., Song, H.J. & Park, C.Y. 2013. Low-cost gel imaging system implementation in reduced size. International Journal of Bio-Science & Bio- Technology 5(5): 151-160.

Lee, D.J., Kim, S.Y., Kim, J.D., Kim, Y.S., Song, H.J. & Park, C.Y. 2014. Reducing the number of UVB lamps for low-cost gel imaging system implementation. International Journal of Control & Automation 7(3): 33-42.

Machado, A., Campos, M., Siqueira, A. & de Carvalho, O. 1997. An iterative algorithm for segmenting lanes in gel electrophoresis images. Proc. X Brazilian Symposium on Computer Graphics and Image Processing. pp. 140-146.

Porch, T.G. & Erpelding, J.E. 2006. Low-cost conversion of the Polaroid MD-4 land camera to a digital gel documentation system. Journal of Biochemical and Biophysical 67(1): 1-5.

Salm, E., Liu, Y.S., Marchwiany, D., Morisette, D., He, Y., Razouk, L., Bhunia, A.K. & Bashir, R. 2011. Electrical detection of dsDNA and polymerase chain reaction amplification. Biomedical Microdevices 13(6): 973-982.

Wu, J., Kodzius, R., Xiao, K., Qin, J. & Wen, W. 2012. Fast detection of genetic information by an optimized PCR in an interchangeable chip. Biomedical Microdevices 14(1): 176-186.

Ye, X., Suen, C.Y., Cheriet, M. & Wang, E. 1999. A recent development in image analysis of electrophoresis gels. Proc. Vision Interface‘99, Trois-Rivières. pp. 432-438.

 

*Corresponding author; email: cypark@hallym.ac.kr

 

 

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