Sains Malaysiana 44(12)(2015): 1739–1744

 

Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic Acid by Supercritical Carbon Dioxide Technology using Response Surface Methodology

(Pengoptimuman Kaedah Penyediaan Berterusan Arthrospira platensis γ Asid Linolenik oleh Teknologi Supergenting Karbon Dioksida yang menggunakan Kaedah Gerak Balas Permukaan)

 

YAO-CHING HSUEH1,2, BE-JEN WANG1, ZER-RAN YU3, CHENG-CHI WANG4 & MALCOLM KOO*5

 

1Department of Food Science, National Chiayi University, Chiayi 60004, Taiwan, ROC

 

2Biotechnology Research Center, Far East University, Tainan 74448, Taiwan, ROC

 

3Superwell Biotechnology Corporation, Taichung 40876, Taiwan, ROC

 

4Graduate Institute of Precision Manufacturing, National Chin-Yi University of Technology, Taichung 41170, Taiwan, ROC

 

5Department of Medical Research, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi 62247, Taiwan, ROC

 

Received: 25 August 2014/Accepted: 23 June 2015

 

ABSTRACT

γ-linolenic acid is an essential omega-6 unsaturated fatty acid made in the human body from linoleic acid. It can be metabolized to various important eicosanoids and it is also a precursor of prostaglandin E and several other active substances that are associated with anti-inflammatory properties. Arthrospira platensis is known to contain relatively large quantities of γ-linolenic acid. The aim of this study was to investigate the optimal parameters under a continuous preparation method of γ-linolenic acid from A. platensis using supercritical carbon dioxide technology. A Box-Behnken experimental design and response surface methodology were used to optimize combinations among pressure (10, 20 and 30 MPa), temperature (40, 50 and 60°C) and flow rate of A. platensis extract liquor (1, 2 and 3 mL/min) for yield of γ-linolenic acid. The results showed that the extraction of γ-linolenic acid from A. platensis was optimized at a temperature of 60°C, a pressure of 30 MPa and a flow rate of 3 mL/min. These parameters could be used as a basis for facilitating future scale-up industrial applications.

 

Keywords: Arthrospira platensis; Spirulina platensis; supercritical carbon dioxide fractionation; γ-linolenic acid

 

ABSTRAK

Asid linolenik γ adalah asid lemak tak tepu penting omega-6 yang dihasilkan dalam tubuh manusia daripada asid linolik. Ia boleh dimetabolismakan untuk pelbagai eicosanoid penting dan ia juga merupakan pelopor prostaglandin E dan beberapa bahan aktif lain yang berkaitan dengan sifat anti-radang. Arthrospira platensis diketahui mengandungi kuantiti asid Linolenik γ yang agak besar. Kajian ini bertujuan untuk mengkaji parameter optimum dengan kaedah penyediaan berterusan asid Linolenik γ daripada A. platensis menggunakan teknologi supergenting karbon dioksida. Satu reka bentuk eksperimen Box-Behnken dan kaedah gerak balas permukaan digunakan untuk mengoptimumkan gabungan antara tekanan (10, 20 dan 30 MPa), suhu (40, 50 dan 60°C) serta kadar aliran cecair ekstrak A. platensis (1, 2 dan 3 mL/min) untuk hasil asid Linolenik γ. Keputusan menunjukkan pengekstrakan asid linolenik γ daripada A. platensis dioptimumkan pada suhu 60°C, tekanan pada 30 MPa dan kadar aliran 3 mL/min. Parameter ini boleh digunakan sebagai asas bagi tujuan memberi kemudahan kepada aplikasi industri pada masa hadapan.

 

Kata kunci: Arthrospira platensis; Asid linolenik γ; pemeringkatan supergenting karbon dioksida; Spirulina platensis

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*Corresponding author; email: m.koo@utoronto.ca

 

 

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