Sains Malaysiana 45(10)(2016): 1517–1523

 

Evaluation of Antihyperglycemic and Antihyperlipidemic Potential of Nelumbo nucifera Seeds in Diabetic Rats

(Penilaian Potensi Antihiperglisemik dan Antihiperlipidemik Biji Nelumbo nucifera dalam Tikus Diabetis)

 

SHAHNAZ1, HIDAYATULLAH KHAN¹*, FARMAN ALI², AFZAL SHAH¹, FARHAT KAMRAN³ & SARWAT JAHAN⁴

¹Department of Chemistry, University of Science and Technology, Bannu (28100) KPK

Pakistan

²Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal, Dir (18000) KPK

Pakistan

 

³Department of Health Sciences, Liberty University, Lynchburg, VA, United States of America

 

⁴Department of Animal Sciences, Quaid-e-Azam University, Islamabad (41000), Pakistan

 

Received: 24 January 2016/Accepted: 6 March 2016

 

ABSTRACT

The present study was aimed at evaluating antihyperglycemic and antihyperlipidemic activity of nuciferin and norcoclaurine constituents of N. nucifera seeds, a well-known medicinal plant. The alloxan (100 mg/kg b.w) induced diabetic rats (200-250 g) were divided into seven groups (n = 6). Group I; normal control, Group II; diabetic control, Group III; standard, Group lV-VII were fed with methanolic crude extracts (100, 200 mg/kg), nuciferin and norcoclaurine (10 mg/kg b.w.), received for 15 days in dose dependent manner. The study included different parameters; examination of oral glucose, fasting blood glucose, serum lipid profile and checking for body weight changes. In oral glucose examination, within 60 and 80 min of treatment, extracts, nuciferin and norcoclaurine significantly reduced blood glucose (p<0.05) and restored body weight in diabetic rats. Alloxan- induced diabetic rats showed 30-50% reduction of blood glucose level (p<0.05) and recovered 5-20% body weight at day 15 after ingestion of crude extracts (100-200 mg/kg b.w.); and nuciferin and norcoclaurine (each at 10 mg/kg b.w.). It also recovered significantly elevated biochemical parameters such as triglycerides (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), total cholesterol (TC), serum urea and creatinine. Our findings indicated that N. nucifera seeds possess significant antihyperglycemic and antihyperlipidemic activity in diabetic rats.

 

Keywords: Antihyperglycemic; antihyperlipidemic; norcoclaurine; nuciferin; N. nucifera

 

ABSTRAK

Penyelidikan ini bertujuan untuk menilai aktiviti antihiperglisemik dan antihiperlipidemik juzuk nusiferin dan norkoklaurin biji N. nucifera, tumbuhan ubatan yang terkenal. Aloksan (100 mg/kg kW) tikus teraruh diabetis (200-250 g) dibahagikan kepada tujuh Kumpulan (n=6). Kumpulan I; kawalan biasa, Kumpulan II; kawalan diabetis, Kumpulan III; piawai, Kumpulan IV-VII diberi makan dengan ekstrak mentah metanolik (100, 200 mg/kg), nusiferin dan norkoklaurin (10 mg/kg b.w.), menerima dos secara bersandar selama 15 hari. Kajian ini merangkumi parameter yang berbeza; pemeriksaan glukosa secara oral, glukosa darah puasa, profil lipid serum dan pemeriksaan perubahan berat badan. Dalam tempoh 60 dan 80 min rawatan untuk pemeriksaan glukosa secara oral, ekstrak, nusiferin dan norkoklaurin telah mengurangkan glukosa darah (p<0.05) dan memulihkan berat badan tikus diabetis. Aloksan - tikus teraruh diabetis menunjukkan 30-50% pengurangan paras glukosa darah (p<0.05) dan mendapat kembali 5-20% berat badan pada hari ke-15 selepas ketakhadaman ekstrak mentah (100-200 mg/kg b.w.); serta nusiferin dan norkoklaurin (masing-masing pada 10 mg/kg b.w.). Ia juga dengan ketara mendapat kembali peningkatan parameter biokimia seperti trigliserida (TG), lipoprotein ketumpatan rendah (LDL), lipoprotein ketumpatan tinggi (HDL), jumlah kolesterol (TC), serum urea dan kreatinina. Keputusan kajian kami menunjukkan bahawa benih N. nucifera mempunyai aktiviti antihiperglisemik dan antihiperlipidemik yang penting dalam tikus diabetis.

 

Kata kunci: Antihiperglisemik; antihiperlipidemik; norkoklaurin; nusiferin; N. nucifera

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*Corresponding author; email: hidayat_sks@yahoo.com