Sains Malaysiana 45(3)(2016): 411–416

Collagen Binding Activity of Bacteria Isolated from Pig and Cow Small Intestine

(Aktiviti Pengikatan Kolagen Bakteria Dipencilkan daripada Usus Kecil Babi dan Lembu)

 

NUR FADHILAH KHAIRIL MOKHTAR1,2*, RAHA ABDUL RAHIM2, AMALIA MOHD HASHIM2 & SHUHAIMI MUSTAFA1,2

 

1Halal Products Research Institute, Putra Infoport, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

 

2Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

 

Received: 31 March 2015/Accepted: 28 August 2015

 

ABSTRACT

Bacterial adherence to connective tissue, especially to collagen has been vastly known for their invasive and infectious activities. However, the ability to exploit the unique and specific interactions between bacteria and collagen as a novel approach in detection of placental collagen has never been explored. This study aimed to determine bacteria with binding specificity to placental collagen (Type IV) derived from human and sheep. In order to do this, total bacteria from small intestines of pig and cow were isolated and their ability to bind to Type IV placental collagen (human and sheep) was determined. Interestingly, three bacterial samples; P5, P9 (pig small intestine origin) and B7 (cow small intestine origin) were found to be able to bind strongly to the placental collagen. The bacterial binding to human placental collagen was however, diminished after the bacteria were treated with trypsin, proteinase K (for removal of surface protein) and guanidine hydrochloride (for S-layer removal), suggesting that the interaction of these bacteria to placental collagen was promoted by protein(s) present at the bacterial surface. In addition, significant reduction of placental collagen-binding ability of the bacteria pre-incubated with soluble human placental collagen showed that there is a specific interaction between the bacteria and collagen. P5, P9 and B7 bacteria were found to share 95-97% 16S rRNA sequence similarity to Enterococcus faecalis ZL, Enterococcus hirae ss33b and Enterococcus faecium M3-1, respectively. The results presented here may facilitate future studies in identifying bacterial surface protein(s) responsible for the specific binding of bacteria to collagen and opens new opportunity to utilize the protein(s) for the detection of placental collagen in nutraceutical and food supplements.

 

Keywords: Bacterial binding; placental collagen; surface protein

 

ABSTRAK

Pelekatan bakteria kepada tisu penghubung, terutamanya kolagen telah diketahui dengan meluas untuk aktiviti invasif mereka. Walau bagaimanapun, dalam kajian ini, keupayaan untuk mengeksploitasi interaksi yang unik dan khusus antara bakteria dan kolagen sebagai pendekatan baru dalam pengesanan kolagen plasenta telah dikaji. Bakteria daripada usus kecil khinzir dan lembu telah diasingkan dan keupayaan mereka untuk mengikat kepada kolagen plasenta ‘Type’ IV (manusia dan biri-biri) telah dikenal pasti. Menariknya, tiga sampel bakteria; P5, P9 (daripada usus kecil babi) dan B7 (daripada usus kecil lembu) didapati mampu mengikat teguh kepada kolagen plasenta. Bagaimanapun, keupayaan bakteria untuk mengikat plasenta kolagen manusia berkurangan selepas bakteria dirawat dengan tripsin, proteinase K (untuk pembuangan protein permukaan) dan guanidin hidroklorida (untuk pembuangan lapisan S). Ini menunjukkan bahawa interaksi bakteria untuk kolagen plasenta dipromosikan oleh protein yang wujud pada permukaan bakteria. Selain itu, pengurangan yang ketara daripada keupayaan bakteria untuk mengikat kolagen plasenta selepas dieram dengan kolagen larut plasenta menunjukkan bahawa terdapat interaksi tertentu antara bakteria dan kolagen. Bakteria P5, P9 dan B7 didapati mempunyai 95-97% persamaan daripada segi jujukan 16S rRNA masing-masing dengan Enterococcus faecalis ZL, Enterococcus hirae ss33b dan Enterococcus faecium M3-1. Hasil daripada kajian ini boleh digunakan sebagai asas kajian lanjut untuk mengenal pasti protein permukaan bakteria tertentu yang bertanggungjawab mengikat kolagen dan membuka peluang baru untuk menggunakan protein tersebut bagi pengesanan kolagen plasenta dalam makanan tambahan dan nutraseutikal.

 

Kata kunci: Kolagen plasenta; pengikatan bakteria; protein permukaan

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*Corresponding author; email: nur021057@gmail.com

 

 

 

 

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