Sains Malaysiana 45(7)(2016): 1049–1055

Lactobacillus plantarum G1 Microencapsulation enhanced its Viability during Storage and Gastrointestinal Transit

(Pemikrokapsulan Lactobacillus plantarum G1 Mempertingkatkan Kebolehhidupannya semasa Storan dan Transit Gastrousus)

HOURIA OULED-HADDAR^1,2*, MOHAMED SIFOUR^1,2, TAYEB IDOUI^1,3, HAMIDA BOURIDANE¹ & SOMIA ARID¹

¹Department of Cell and Molecular Biology, Faculty of Nature and Life Sciences, University of Jijel,

Algeria

²Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel,

Algeria

³Laboratory of Biotechnology, Health and environment, Faculty of Nature and Life Sciences

University of Jijel, Algeria

Received: 22 December 2014/Accepted: 27 January 2016

ABSTRACT

A Lactobacillus plantarum strain G1 was previously isolated from chicken crop and it was found to show interesting probiotic properties. In this study, several microencapsulation combined materials were used to test their ability to protect cells from gastrointestinal harsh conditions. The results on kinetics of cell release after exposition to acidic pH (2.0) indicated that the efficiency of cell entrapment of the gels was as follows starting from the highest capacity to the lowest one: sodium alginate, alginate-agar (AA), alginate-starch (AS) and κ-carrageenan. In addition, effect of bile salts on cell release was also tested; the results showed that the efficiency of entrapment of the gels was different and as follows starting from the highest capacity to the lowest one: AA, sodium alginate, κ-carrageenan, and AS. Moreover, viability of free and alginate-microencapsulated Lactobacillus plantarum after exposure to acid, bile and pancreatic enzymes was investigated; the results showed that microencapsulation was capable of protecting cells against the harsh conditions of the gastrointestinal tract. In addition, microencapsulation enhanced the viability of Lb. plantarum during cold storage for 2 weeks and during a range of heat treatments.

Keywords: Gastrointestinal conditions; Lactobacillus plantarum; microencapsulation; probiotics

ABSTRAK

Strain G1 Lactobacillus plantarum diasingkan daripada tembolok ayam dan ia didapati menunjukkan sifat probiotik yang menarik. Dalam kajian ini, beberapa bahan gabungan pemikrokapsulan telah digunakan untuk menguji keupayaan mereka untuk melindungi sel daripada keadaan yang teruk dalam gastrousus. Keputusan pembebasan sel kinetik selepas pendedahan kepada pH berasid (2.0) menunjukkan bahawa kecekapan pemerangkapan sel oleh gel adalah seperti berikut; bermula daripada kapasiti tertinggi ke terendah: natrium alginat, alginat-agar (AA), alginat-kanji (AS) dan κ-carrageenan. Di samping itu, kesan garam hempedu ke atas pembebasan sel juga telah diuji; keputusan menunjukkan bahawa kecekapan pemerangkapan gel adalah berbeza dan seperti berikut bermula daripada kapasiti tertinggi ke terendah: AA, natrium alginat, κ-carrageenan dan AS. Selain itu, kebolehhidupan Lactobacillus plantarum yang bebas dan alginat-pemikrokapsulan selepas terdedah kepada asid, hempedu dan enzim pankreas dikaji; hasil kajian menunjukkan bahawa pemikrokapsulan mampu melindungi sel terhadap keadaan yang teruk dalam saluran gastrousus. Di samping itu, pemikrokapsulan meningkatkan kebolehhidupan Lb. plantarum semasa penyimpanan sejuk selama 2 minggu dan semasa pelbagai rawatan haba.

Kata kunci: Keadaan gastrousus; Lactobacillus plantarum; pemikrokapsulan; probiotik

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*Corresponding author; email: hrourou2002@gmail.com