Sains Malaysiana 46(9)(2017): 1557–1563

http://dx.doi.org/10.17576/jsm-2017-4609-26

 

Acrylamide Optical Sensor Based on Hydrolysis Using Bacillus sp. Strain ZK34 Containing Amidase Properties

(Sensor Optik Akrilamida Berasaskan Hidrolisis Menggunakan Bacillus sp. Strain ZK34 yang Mengandungi Sifat Amidase)

 

YEE-MAY CHONG¹, MUSA AHMAD^1,2*, LEE YOOK HENG¹, NORZILA KUSNIN³ & MOHD YUNUS ABDUL SHUKOR³

 

¹School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

²Industrial Chemical Technology Programme, Faculty of Sciences and Technology

Universiti Sains Islam Malaysia, 71800 USIM Nilai, Negeri Sembilan Darul Khusus, Malaysia

 

³Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

 

Received: 16 January 2017/Accepted: 11 May 2017

 

 

ABSTRACT

In this work, a new optical screening method for acrylamide was developed. Bacterial Bacillus sp. strain ZK 34 was used to hydrolyse acrylamide to the corresponding acid and ammonia. Nessler's reagent was used to detect the produced ammonia and the yellow complex formed was treated as signal. Bacterial pellet was immobilised in the alginate membrane. The optimum composition of alginate used is 2%. The mass ratio of alginate:bacterial of 1:0.5 gave the optimum respond. Optimum concentration for NaOH and Nessler's reagent were 0.075 M and 2.5 mM, respectively. The yellow complex of mercury (II) amido-iodine formed was directly proportional to the concentrations of acrylamide up to 50.00 ppm with the limit of detection of 1.30 ppm. This sensor shows a good reproducibility which the relatives standard deviation (RSD) values from 3.17-6.15%. Therefore, the detection of acrylamide based on the amidase hydrolysis is suitable for screening this carcinogen compound.

 

Keywords: Acrylamide; amidase; Nessler's reagent; optical detection

 

ABSTRAK

Dalam kajian ini, satu kaedah baharu untuk penyaringan akrilamida secara optik telah dibangunkan. Bakteria Bacillus sp. strain ZK 34 telah digunakan untuk menghidrolisiskan akrilamida kepada asid yang sepadan dan amonia. Reagen Nessler telah digunakan untuk mengesan amonia yang terhasil dan pembentukan sebatian kuning diambil kira sebagai isyarat. Palet bakteria telah dipegunkan di dalam membran alginat. Komposisi alginat yang optimum digunakan ialah 2%. Nisbah jisim alginat:bakteria pada 1:0.5 memberi rangsangan yang optimum. Kepekatan NaOH dan reagen Nessler yang optimum masing-masing ialah 0.075 M dan 2.5 mM. Sebatian kuning iaitu raksa (II) amido-iodin yang terbentuk berkadar langsung dengan kepekatan akrilamida sehingga 50.00 ppm dengan had pengesanan 1.30 ppm. Sensor ini menunjukkan kebolehulangan yang baik iaitu sisihan paiwai relatif (RSD) daripada 3.17-6.15%. Maka pengesanan akrilamida berasaskan hidrolisis amidase adalah sesuai untuk penyaringan sebatian yang karsinogen ini.

 

Kata kunci: Akrilamida; amidase; pengesanan optik; reagen Nessler

 

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*Corresponding author; email: andong@usim.edu.my