Sains Malaysiana 47(10)(2018): 2337–2347

http://dx.doi.org/10.17576/jsm-2018-4710-10

 

Neuroprotective Effects of Ethyl Acetate Extract of Zingiber zerumbet (L.) Smith against Oxidative Stress on Paraquat-Induced Parkinsonism in Rats

(Kesan Neurolindungi Ekstrak Etil Asetat Zingiber zerumbet (L.) Smith terhadap Tekanan Oksidatif pada Tikus Parkinsonisme Aruhan Parakuat)

 

FARAH WAHIDA IBRAHIM, UMI NORAASHIKIN ZAINUDIN, MAZLYZAM ABDUL LATIF & ASMAH HAMID*

 

Biomedical Science Programme, Center for Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia

 

Received: 14 March 2018/Accepted: 5 June 2018

 

ABSTRACT

Zingiber zerumbet has been traditionally used as an anti-inflammation and antioxidant agent. The present study investigates the neuroprotective effects of ethyl acetate extract of Z. zerumbet against oxidative stress on paraquat (PQ)-induced Parkinsonism in rats. Forty male Sprague-Dawley rats were divided into five groups: Negative control (normal saline), positive control (N-acetylcysteine, NAC 20 mg/kg + PQ 10 mg/kg), PQ only, 200 mg/kg Z. zerumbet + PQ and 400 mg/kg Z. zerumbet + PQ. The extract was given orally for 19 consecutive days and PQ was administered intraperitoneally on day 8-12th of the treatment regime. Both serum and fresh brains containing substantia nigra (SN) region were taken for biochemical and histological analysis. Administration of both 200 and 400 mg/kg ethyl acetate Z. zerumbet extracts to the PQ-treated groups have resulted in: Decreased levels of MDA and PC in the SN homogenates; and increased SOD, GPx; and CAT activities in the SN and serum. Overall, ethyl acetate extract of Z. zerumbet reduced oxidative stress in the SN of PQ-induced neuronal damages, therefore, has the potential to be developed as a preventive agent for neurodegenerative disorders caused by environmental toxins.

 

Keywords: Antioxidant; oxidative stress; paraquat; Parkinson’s disease; Zingiber zerumbet

 

ABSTRAK

Zingiber zerumbet telah digunakan secara tradisi sebagai agen anti-keradangan dan antioksidan. Kajian ini dijalankan untuk mengkaji kesan neurolindungi ekstrak etil asetat Z. zerumbet terhadap tekanan oksidatif dalam tikus Parkinsonisme aruhan parakuat (PQ). Empat puluh tikus Sprague-Dawley jantan dibahagikan kepada lima kumpulan: Kawalan negatif (salin normal), kawalan positif (N-asetilcysteine, NAC 20 mg/kg + PQ 10 mg/kg), PQ sahaja, 200 mg/kg Z. zerumbet + PQ dan 400 mg/kg Z. zerumbet + PQ. Ekstrak diberikan secara oral gavaj selama 19 hari berturut-turut dan PQ diberikan secara intraperitoneal pada hari ke-8 sehingga ke-12 rejim rawatan. Kedua-dua sampel serum dan tisu otak pada bahagian substantia nigra (SN) diambil untuk analisis biokimia dan histologi. Pemberian ekstrak etil asetat Z. zerumbet pada kedua-dua dos 200 dan 400 mg/kg kepada kumpulan yang dirawat PQ telah: Menurunkan tahap MDA dan PC dalam homogenat SN; meningkatkan aktiviti SOD, GPx, dan CAT dalam SN dan serum; serta meningkatkan aras glutation terturun (GSH) dalam SN. Secara keseluruhannya, ekstrak etil asetat Z. zerumbet didapati dapat mengurangkan tekanan oksidatif pada kawasan SN di otak tikus yang diaruh kerosakan neuron oleh PQ. Seterusnya, berpotensi untuk dibangunkan sebagai agen pencegah gangguan penyakit neurodegeneratif yang disebabkan oleh pendedahan terhadap toksin dari alam sekitar.

 

Kata kunci: Antioksidan; parakuat; penyakit Parkinson; tekanan oksidatif; Zingiber zerumbet

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*Corresponding author; email: asmah0901@ukm.edu.my

 

 

 

 

 

 

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