Sains Malaysiana 47(10)(2018): 2411–2419

http://dx.doi.org/10.17576/jsm-2018-4710-17

 

Kesan Suplementasi Tokotrienol ke atas Perubahan Hormon dan Katekolamin Otak Tikus Aruhan Stres

(Effect of Tocotrienol Supplementation on Hormones and Catecholamines in the Brain of Rats Exposed to Stress)

 

NUR AZLINA MOHD FAHAMI1*, NADDIAH SYAFIQAH RAMLI2 & ELDA SURHAIDA LATIF2

 

1Jabatan Farmakologi, Fakulti Perubatan, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

2Program Bioperubatan, Fakulti Sains Bersekutu, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

Received: 30 March 2018/Accepted: 5 June 2018

 

 

ABSTRAK

Stres merupakan sebarang gangguan, cabaran atau ancaman kepada seseorang individu yang merangsang perubahan respon fizikal, mental dan emosi. Hormon stres dan katekolamin dirembeskan di dalam otak sebagai respon terhadap stres. Kajian ini mengenal pasti kesan tokotrienol tulen dan vitamin E sawit ke atas perubahan hormon stres dan katekolamin pada tikus yang diaruhkan stres imobilisasi rendaman air. Sebanyak 32 ekor tikus Wistar jantan telah dibahagikan secara rawak kepada empat kumpulan kajian. Dua kumpulan kawalan; kumpulan tanpa stres (NS) dan kumpulan tanpa stres (CS) serta dua kumpulan rawatan yang diberikan tokotrienol (TTS) atau vitamin E sawit (TFS) secara oral paksaan pada dos 60 mg/kg berat badan selama 28 hari. Setelah tamat tempoh rawatan, tikus daripada kumpulan CS, TTS dan TFS telah didedahkan kepada stres imobilisasi rendaman air selama tiga setengan jam. Kemudian, otak tikus diambil untuk pengukuran hormon pelepasan kotikotrofin (CRH), hormon adenokortikotropik (ACTH), norepinefrin, dopamin dan serotonin. Keputusan kajian mendapati peningkatan kandungan CRH, ACTH, norepinefrin dan dopamine di dalam otak tikus yang diaruh stres berbanding kumpulan kawalan tanpa stres. Kandungan serotonin pula menurun akibat stres. Walau bagaimanapun, tikus yang diberi suplementasi tokotrienol dan vitamin E sawit menunjukkan penurunan signifikan kandungan CRH, ACTH, norepinefrin dan dopamin serta peningkatan kandungan dan serotonin sehingga hampir ke aras normal. Tiada perbezaan antara pemberian tokotrienol dan vitamin E sawit. Sebagai kesimpulan, tokotrienol dan vitamin E sawit berkesan dalam mengawal perubahan hormon stres serta katekolamin pada otak tikus yang teraruh stres.

 

Kata kunci: Hormon stres; katekolamin; otak; stres restrain; tokotrienol

 

ABSTRACT

Stress includes any disturbances, challenges or threats to an individual, which stimulates physical, mental and emotional responses. Stress hormones and catecholamines are released from the brain in response to stress. Antioxidant properties of vitamin E had been proven to help in reducing stress. This study was designed to investigate the effects of tocotrienol and palm vitamin E supplementation on stress hormones and catecholamines in stress-induced rats. Thirty two male Wistar rats were randomly assigned into four groups, consisted of two control groups (NS and CS) which were fed with a commercially prepared normal rat diet while two treatment groups (TTS and TFS) were given tocotrienol or palm vitamin E orally in the dose of 60 mg/kg body weight. After 28 days of treatment; CS, TTS and TFS rats were subjected to water-immersion restraint stress for three and a half hours once. The rats were killed and their brain was taken to determine stress hormones and catecholamines levels. The findings showed that stressed (CS) rats had a significantly higher levels of CRH, ACTH, norepinephrine and dopamine and lower level of serotonin in the brain. Interestingly, supplementation with both tocotrienol and palm vitamin E were able to significantly reduced the CRH, ACTH, norepinephrine and dopamine levels. No difference was observed when comparing the effects of tocotrienol with palm vitamin E. In conclusion, tocotrienol and palm vitamin E are capable in reducing changes to the stress hormones and catecholamines in stress-induced rats.

 

Keywords: Brain; catecholamines; palm vitamin E; restraint stress; stress hormones; tocotrienol

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*Corresponding author; email: nurazlinamf@ukm. edu.my

 

 

 

 

 

 

 

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