Sains Malaysiana 47(10)(2018): 2491–2499

http://dx.doi.org/10.17576/jsm-2018-4710-26

 

Total Nucleated Cell Count and CD34+ Cell is Reduced in Preeclampsia and Gestational Diabetes Mellitus Pregnancies: Viable Affix Criteria for Cord Blood Banking

(Jumlah Sel Bernuklues dan Sel CD34+ Berkurangan dalam Kehamilan Praeklampsia dan Gestati Diabetes Mellitus: Kriteria Viabel Afiks untuk Perbankan Darah Tali Pusat)

 

MOHD RAZIF MOHD IDRIS1, FAZLINA NORDIN1*, ZALEHA ABDULLAH MAHDY2 & S. FADILAH ABD WAHID1

 

1Cell Therapy Centre (CTC), Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

2Department of Obstetrics and Gynaecology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

Received: 6 March 2018/Accepted: 20 June 2018

 

ABSTRACT

The aim of this study to determine the numbers of CD34+ cells and total nucleated cell (TNC) in umbilical cord blood (UCB) collected from pregnant mothers with gestational diabetes mellitus (GDM) and preeclampsia (PE), following statistical analysis of both maternal and perinatal factors which affect UCB parameters. Most of studies explored the influence of obstetric factors on the number of UCB cell collection and only a few looked at the effects on UCB haematopoietic stem cell (UCB-HSC) of common disorders complicating pregnancy. A total of 112 UCB samples (32 PE, 42 GDM and 38 non-diseased) were collected. CD34+ cell and NC count were enumerated using FACS Calibur. The TNC and CD34+ cells were significantly reduced in both PE and GDM groups as compared to the control group. The PE group shows significantly lower birth weight and higher BP which led to a lower UCB volume and CD34+ count. Gestational age shows significant correlation with nucleated cell count (NCC) and TNC. GDM group shows significantly lower systolic BP, NCC and TNC count, including low placental weight and birth weight. Conclusively, some obstetrics factors have significant influences to the numbers and quality of UCB-HSC in both PE and GDM groups, which could guide in the selection criteria for CB banking.

 

Keywords: CD34+ cells; gestational diabetes mellitus (GDM); haematopoietic stem cell (HSC); preeclampsia (PE); total nucleated cell (TNC); umbilical cord blood (UCB)

 

ABSTRAK

Tujuan kajian ini adalah untuk menentukan bilangan sel CD34+ dan jumlah sel bernukleus (TNC) dalam darah tali pusat (UCB) ibu mengandung dengan diabetes mellitus (GDM) dan praeklampsia (PE), serta analisis statistik faktor maternal dan perinatal yang mempengaruhi parameter UCB. Kebanyakan kajian mengkaji pengaruh faktor obstetrik pada bilangan sel UCB dan hanya beberapa kajian yang melihat kesan penyakit semasa kehamilan pada kualiti UCB-HSC. Sebanyak 112 sampel UCB (32 PE, 42 GDM dan 38 normal) diperoleh. Bilangan sel CD34+ dan TNC dihitung menggunakan FACS Calibur. Bilangan TNC dan sel CD34+ adalah lebih rendah dalam kumpulan PE dan GDM berbanding kumpulan normal. Kumpulan PE menunjukkan berat bayi yang lebih rendah dan BP yang lebih tinggi yang menyebabkan jumlah isi padu UCB dan bilangan sel CD34+ yang lebih rendah. Faktor umur kehamilan menunjukkan korelasi yang signifikan dengan bilangan NC dan TNC. Kumpulan GDM menunjukkan BP sistolik, NC dan TNC yang lebih rendah, termasuk berat plasenta dan berat bayi yang rendah. Kesimpulannya, beberapa faktor obstetrik mempengaruhi bilangan dan kualiti UCB-HSC secara signifikans pada kedua-dua kumpulan PE dan GDM yang dapat membantu di dalam kriteria pemilihan untuk perbankan CB.

 

Kata kunci: Darah tali pusat (UCB); jumlah sel bernukleus (TNC); kehamilan diabetes mellitus (GDM); praeklampsia (PE); sel CD34+; sel stem hematopoitik (HSC)

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*Corresponding author; email: nordinf@ppukm.ukm.edu.my

 

 

 

 

 

 

 

 

 

 

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