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http://dx.doi.org/10.17576/jsm-2018-4712-13
Comparative Analysis of Metabolites and
Antioxidant Potentials from Different Plant Parts of Curcuma
aeruginosa Roxb. (Analisis Bandingan Kandungan Metabolit dan Potensi
Antioksidan daripada Bahagian Berbeza Curcuma aeruginosa
Roxb.)
SANIMAH SIMOH*, SEW YUN SHIN, FAZRI ABD RAHIM, MUHAMMAD AIZUDDIN AHMAD
& ALIZAH ZAINAL
Biotechnology
and Nanotechnology Research Centre, Malaysian Agricultural Research and
Development Institute (MARDI), Persiaran MARDI-UPM, 43400 Serdang, Selangor
Darul Ehsan
Malaysia
Received: 30 May 2018 /Accepted:
13 September 2018
ABSTRACT
A comparative analysis of
metabolites from different parts of Curcuma aeruginosa, i.e. leaves,
stems, adventitious roots and rhizomes was performed by GC-MS/MS coupled
with multivariate statistical analysis. The GC-MS/MS analysis
confirmed the occurrence of 26 metabolites belonged to terpenoids in almost all
the samples. The Principal Component Analysis (PCA)
indicated that there was a clear distinction between rhizomes and other plant
parts, i.e. stems, leaves, and adventitious roots that could be explained by
relatively higher contents of terpenoids including curzerene,
alpha-farnesen, furanocoumarin, velleral, germacrone cineole, borneol, beta- and gamma- elemene and methenolone. The results of Hierarchical
Clustering Analyses (HCA) corresponded with the PCA results
where many terpenoids found abundantly high in rhizome were clustered together.
This was supported by the Pearson correlation analysis that showed a
significantly good relationship between those terpenoids. The adventitious
roots demonstrated the strongest antioxidant activity as compared to the other
plant parts which could be attributed to its highest Total Phenolic Contents (TPC).
Total phenolic contents of all the plant parts were positively correlated with
their antioxidant activities which indicate that phenolic compounds may play a
role in the overall antioxidant activities of the plants. The results of the
study highlighted the potential of this underexploited Curcuma species
which could serve as a new source of important phytochemicals and natural
antioxidant that could be incorporated in functional foods and nutraceuticals.
In addition, chemical and biological evidence shown in the present work has
rationalised the different uses of various plant parts of C. aeruginosa.
Keywords: Antioxidant; Curcuma
aeruginosa; GC-MS/MS; metabolomics
ABSTRAK
Suatu analisis perbandingan metabolit
daripada bahagian berlainan Curcuma aeruginosa, iaitu daun,
batang, akar adventisius dan rizom dijalankan dengan menggunakan
GC-MS/MS
berserta dengan analisis statistik multivariasi. Analisis GC-MS/MS mengesahkan kehadiran 26 metabolit
dalam hampir kesemua sampel dengan kebanyakan metabolit tergolong
dalam kumpulan terpenoid. Analisis Komponen Utama (PCA)
menunjukkan terdapat perbezaan ketara metabolit antara rizom dan
bahagian tumbuhan lain seperti batang, daun dan akar adventisius
yang dapat dijelaskan dengan kandungan yang tinggi terpenoidnya,
termasuk curzerene, alfa-farnesen, furanocoumarin, velleral,
germacrone cineole, borneol, beta- dan gama-elemene dan
methenolone. Keputusan analisis pengklusteran
hierarki (HCA) adalah selari dengan keputusan
PCA dengan sebahagian besar terpenoid yang didapati di dalam
rizom dikluster bersama. Ini disokong
oleh analisis korelasi Pearson yang menunjukkan perkaitan yang signifikan
antara terpenoid berkenaan. Akar adventisius menunjukkan
aktiviti antioksidan tertinggi berbanding dengan bahagian tumbuhan
yang lain yang disebabkan oleh kandungan
keseluruhan fenoliknya yang paling tinggi. Jumlah kandungan keseluruhan
fenolik (TPC) daripada semua bahagian tumbuhan
berkolerasi secara positif dengan aktiviti antioksidan (DPPH,
FRAP)
yang menunjukkan bahawa sebatian fenolik mungkin memainkan peranan
penting dalam menyumbang kepada aktiviti antioksidan tumbuhan. Keputusan
kajian menekankan potensi spesies Curcuma yang kurang dieksploitasi
ini untuk menjadi satu sumber baru fitokimia dan antioksidan semula
jadi yang penting yang boleh digunakan dalam makanan fungsian dan
nutraseutik. Tambahan lagi, bukti kimia dan
biologi yang diperoleh dalam kajian ini memberikan rasional kepada
penggunaan berbeza pelbagai bahagian tumbuhan C. aeruginosa.
Kata
kunci: Antioksida; Curcuma aeruginosa; GC-MS/MS;
metabolomik
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*Corresponding author; email: sanimah@mardi.gov.my
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