Sains Malaysiana 47(1)(2018): 91–97

http://dx.doi.org/10.17576/jsm-2018-4701-11

Characterization and Screening of Lipolytic Bacteria from Thai Fermented Fish

(Pencirian dan Penyaringan Bakteria Lipolitik daripada Ikan Pekasam Thai)

RUNGSIMA DAROONPUNT¹, NAOTO TANAKA², MASATAKA UCHINO³ & SOMBOON TANASUPAWAT^1*

¹Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand

²NODAI Culture Collection Center (NRIC), Faculty of Applied Bio-Science, Tokyo University of Agriculture, Tokyo 156-8502, Japan

³Department of Applied Biology and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo 156-8502, Japan

Received: 10 August 2015/Accepted: 6 July 2017

ABSTRACT

Nine bacterial strains were isolated from Thai fermented fish by the standard dilution technique using JCM no. 377 medium. The rod shaped isolate FN2-3 was identified as Virgibacillus dokdonensis while isolates FN2-3 and FN6-6 as V. halodenitrificans, FN1-13 as Corynebacterium variabile, FN1-10 as Oceanobacillus iheyensis and FN3-7 was Bacillus amyloliquefaciens subsp. plantarum. The coccal isolates, FN6-1 and FN6-7 were Staphylococcus saprophyticus subsp. bovis and FN6-8 was S. saprophyticus subsp. saprophyticus, based on their phenotypic characteristics and 16S rRNA gene sequence analyses at a 99.63-100% sequence similarity. Their lipase activity in complex medium (CM), CM medium with 1% (v/v) Tween 20 or CM medium with 1% (v/v) Tween 80 ranged from 1.12 ± 0.03-3.77 ± 0.04 unit/mL, with the highest lipase activity found with V. dokdonensis FN1-8 cultivated with CM with 1% (v/v) Tween 80.

Keywords: Fermented fish; halophilic bacteria; lipase; lipolytic bacteria

ABSTRAK

Sembilan strain bakteria telah dipencilkan daripada ikan pekasam Thai melalui teknik kecairan piawai yang menggunakan medium JCM no. 377. Pencilan berbentuk rod FN2-3 dikenal pasti sebagaiVirgibacillus dokdonensis manakala pencilan FN2-3 dan FN6-6 sebagaiV. Halodenitrificans, FN1-13 sebagaiCorynebacterium variabile, FN1-10 sebagai Oceanobacillus iheyensis dan FN3-7 sebagai Bacillus amyloliquefaciens subsp. plantarum. Pencilan kokus FN6-1 dan FN6-7 adalah Staphylococcus saprophyticus subsp. bovis dan FN6-8 S. saprophyticus subsp. saprophyticus, berdasarkan ciri fenotip dan 16S rRNA analisis jujukan gen pada satu persamaan turutan 99.63-100%. Aktiviti lipase mereka dalam medium kompleks (CM), medium CM 1% (v/v) Tween 20 atau CM medium dengan 1% (v/v) Tween 80 berjulat dari 1.12 ± 0.03-3.77 ± 0.04 unit/mL dengan aktiviti lipase tertinggi dilihat padaV. dokdonensis FN1-8 yang ditanam dengan 1% (v/v) Tween 80 CM.

Kata kunci: Bakteria halofili lipase; bakteria lipolitik; ikan pekasam

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*Corresponding author; email: Somboon.T@chula.ac.th