Sains Malaysiana 47(2)(2018): 277-286

http://dx.doi.org/10.17576/jsm-2018-4702-09

 

Production of Phytase by Mitsuokella jalaludinii in Semi-Solid State Fermentation of Agricultural by-products

(Pengeluaran Fitase oleh Mitsuokella jalaludinii dalam Fermentasi Separa Pepejal Produk Sampingan Pertanian)

 

Hooi Chia Tang¹, Chin Chin Sieo¹, Norhani Abdullah^2*, Rosfarizan Mohamad³, Abdul Rahman Omar¹, Chun Wie Chong⁴, Anwar Fitrianto⁵ & Yin Wan Ho¹

 

¹Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

²Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

³Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

⁴Department of Life Sciences, School of Pharmacy, International Medical University, 57000 Bukit Jalil, Kuala Lumpur, Wilayah Persekutuan, Malaysia

⁵Department of Mathematics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

Received: 25 April 2017/Accepted: 2 August 2017

 

 

ABSTRACT

 

Phytase activity and growth of anaerobic rumen bacterium, Mitsuokella jalaludinii were investigated by semi-solid state fermentation. Carbon source (rice bran, yam and cassava), nitrogen sources (soya bean, offal meal, fish meal and feather meal) and growth factors (hemin, L-cysteine hydrochloride and minerals) were evaluated in a one-factor-at-a-time approach. Rice bran and fish meal produced better growth and phytase enzyme activity. The removal of L-cysteine hydrochloride and minerals significantly decreased (p<0.05) phytase activity from 1178.72 U to 446.99 U and 902.54 U, respectively. The response surface methods (RSM) was conducted to optimize the phytase production and the results showed the combination of 7.7% of rice bran and 3.7% of fish meal in semi-solid state fermentation gave the highest phytase activity. Maximum phytase production and optimum growth of bacteria were detected at 12 h incubation in both MF medium (control) and agro-medium. In this agro-medium, M. jalaludinii produced 2.5 fold higher phytase activity compared to MF medium.

 

Keywords: Agro-medium; Mitsuokella jalaludinii; phytase activity; response surface methods; semi-solid state fermentation

 

ABSTRAK

Aktiviti fitase dan pertumbuhan bakteria rumen anaerob, Mitsuokella jalaludinii dikaji dengan teknologi fermentasi separa pepejal. Sumber karbon (dedak beras, keladi dan ubi kayu), sumber nitrogen (kacang soya, mil organ, mil ikan dan mil bulu pelepah) serta faktor pertumbuhan (hemin, L-cystein hidroklorida dan mineral) dinilai dengan kaedah satu faktor pada satu masa. Dedak beras dan mil ikan didapati mampu menghasilkan pertumbuhan sel dan aktiviti fitase yang baik. Penyingkiran L-cysteine hidroklorida dan mineral menyebabkan penurunan aktiviti fitase yang ketara (p<0.05) masing- masing dari 1178.72 U ke 446.99 U dan 902.54 U. Kaedah gerak balas permukaan (RSM) dijalankan untuk mengoptimumkan penghasilan fitase dan keputusan menunjukkan gabungan penggunaan 7.7% dedak beras dan 3.7% mil ikan dalam proses fermentasi separa pepejal memberikan aktiviti fitase yang tertinggi. Penghasilan fitase dan pertumbuhan maksimum bakteria untuk kedua-dua media MF (kawalan) and agro-medium berlaku dalam masa eraman 12 jam. M. jalaludinii berupaya menghasilkan 2.5 kali ganda aktiviti fitase dalam agro-medium berbanding dengan media MF.

 

Kata kunci: Agro-media; aktiviti fitase; fermentasi separa pepejal; kaedah gerak balas permukaan; Mitsuokella jalaludinii

 

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  *Corresponding author; email: norhani.biotech@gmail.com