Sains Malaysiana 47(3)(2018): 427–432

http://dx.doi.org/10.17576/jsm-2018-4703-01

 

Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets

(Peringkat Kematangan Testis Ketam Bakau (Scylla olivacea) Induk ke atas Diet yang Berlainan)

MHD IKHWANUDDIN1*, GHAZALI AZMIE1, SITI FATIMAH NAHAR2, WENDY WEE2, MOHAMAD N AZRA1  & AMBOK-BOLONG ABOL-MUNAFI2

 

1Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

 

2School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

 

Received: 1 August 2016/Accepted: 25 September 2017

 

ABSTRACT

The aim of this study was to investigate how various diets influence testis maturation stages in mud crab (Scylla olivacea) broodstock. Morphological and histological assessments were performed in triplicate (10 male crabs each). Daily, subject crabs were fed a squid (Loligo sp.) and a fish (Decapterus sp.) diet at 5-10% of body weight. Diets were analyzed following methods from the Association of Analytical Communities (AOAC). In comparison to control (wild) crabs, the two diets generally did not cause significant differences (p>0.05) in body weight, carapace width and gonadosomatic index (GSI), except in the GSI of squid-fed crabs (p<0.05). At the end of the experiment, crabs that reached Stage 3 testis maturation included were 6 fish-fed individuals and 23 squid-fed individuals. Additionally, differences in crude protein and fat levels across diets influenced the nature of male gonadal development. In conclusion, a squid diet was sufficient to induce Stage 3 testis maturation in Scylla olivacea within 60 days of culture. Our results should prove the usefulness in developing appropriate feeding regimes for male Scylla olivacea broodstock.

 

Keywords: Association of Analytical Communities; crab reproduction; crustacean; nutrition; testis maturation

 

ABSTRAK

Tujuan penyelidikan ini adalah untuk mengkaji bagaimana pelbagai jenis diet makanan mempengaruhi peringkat kematangan testis ketam bakau (Scylla olivacea) induk. Penilaian morfologi dan histologi dilakukan dalam tiga kali pengulangan (10 ekor induk jantan setiap kali pengulangan). Kesemua ketam diberi makan diet makanan sotong (Loligo sp.) dan ikan (Decapterus sp.) sebanyak 5-10% berat badannya setiap hari. Setiap diet makanan akan dianalisis mengikut kaedah daripada Association of Analytical Communities (AOAC). Secara amnya, jika dibandingkan dengan kumpulan ketam yang dikawal, kedua-dua jenis diet makanan tersebut tidak menunjukkan kesan yang signifikan (p>0.05) ke atas berat badan, saiz ukuran badan dan indeks kematangan gonadnya (GSI), kecuali indeks GSI ke atas ketam yang diberi makan sotong. Pada akhir kajian, hanya 6 ekor ketam yang diberi makan ikan dan 23 ekor ketam yang diberi makan sotong mencapai tahap 3 dalam peringkat kematangan testisnya. Sebagai tambahan, perbezaan antara protein mentah dan paras lemak dalam diet makanan telah mempengaruhi perkembangan gonad jantan secara semula jadi. Sebagai kesimpulan, diet makanan sotong sudah pun mencukupi untuk mendorong tahap 3 kematangan ketam jenis Scylla olivacea selama 60 hari ternakan. Keputusan kami adalah sangat berguna dalam membuktikan bahawa rejim pemakanan yang sesuai perlulah dibangunkan untuk induk jantan ketam Scylla olivacea.

 

Kata kunci: Association of Analytical Communities; kematangan testis; krustasia; nutrisi; pembiakan ketam

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*Corresponding author; email: ikhwanuddin@umt.edu.my