Sains Malaysiana 47(6)(2018): 1139–1146

http://dx.doi.org/10.17576/jsm-2018-4706-09

Sintesis dan Pencirian Kompleks Nikel(II) Salfen dengan Rantai Sisi Piperidina dan Kajian Interaksi dengan DNA Denggi

(Synthesis and Characterization of Nickel(II) Salphen Complex with Piperidine Side

Chain and Interaction Study with Dengue DNA)

EDA YUHANA ARIFFIN¹, LEE YOOK HENG¹, TAN LING LING²

& NURUL HUDA ABD KARIM^1*

¹Pusat Pengajian Sains Kimia & Teknologi Makananm Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

²Penyelidikan Inisiatif Pencegahan Bencana Asia Tenggara (SEADPRI-UKM)

Institut Pembangunan dan Persekitaran (LETARI), Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 15 September 2017/Accepted: 5 February 2018

ABSTRAK

Pembangunan model biosensor DNA berasaskan pengikatan dan interaksi kompleks logam sebagai penanda penghibridan DNA boleh digunakan untuk diagnosis awal virus denggi melalui penghibridan DNA denggi sebagai DNA sasaran. Kompleks nikel(II) salfen dengan rantai sisi piperidina berjaya disintesis dan pencirian dijalankan dengan menggunakan Spektroskopi Magnetik Nukleus (NMR), Spektroskopi inframerah (FTIR), spektrometri jisim, dan kolorimetri pengimbasan pembezaan. Jenis perikatan dan interaksi antara kompleks nikel(II) salfen dengan rantai sisi piperidina dan DNA denggi dikaji dengan pentitratan DNA ultralembayung nampak. Berdasarkan spektrum ultralembayung nampak, hipokromisme dan anjakan merah (batokromisme) diperhatikan apabila kompleks nikel(II) salfen dengan rantai sisi piperidina dititratkan dengan DNA denggi menunjukkan pembentukan kompleks baru. Kompleks nikel(II) salfen dengan rantai sisi piperidina berinteraksi dengan DNA denggi secara perikatan interkalasi dengan pemalar perikatan, Kb= 4.576×105 M-1. Penemuan ini sangat berharga dalam menjadikan kompleks nikel(II) salfen sebagai penanda penghibridan bagi biosensor DNA optik untuk mengesan kehadiran virus denggi.

Kata kunci: DNA denggi; interaksi DNA; kompleks nikel(II) salfen; nikel(II) salfen; pencirian kompleks

ABSTRACT

Development of DNA biosensor model based on interaction of metal complexes as DNA hybridization marker can be used for dengue viruses early diagnosis. Nickel(II) salphen complex with piperidine side chain were successfully synthesized and characterized using Nucleus Magnetic Resonance Spectroscopy (NMR), mass spectrometry, differential scanning calorimetry and FTIR. The binding mode and interactions of nickel(II) salphen complex with piperidine side chain and dengue DNA were determined by UV-vis titration. Based on ultra violet visible spectra, hypochromism and redshift (bathochromism) were observed after upon tiration of the complex with dengue DNA which suggested formation of new complex. Nickel(II) salphen complex with piperidine side chain interact with dengue DNA by intercalation binding mode with the binding constant, Kb= 4.576x105 M-1. This new finding is so valuable for developing nickel(II) salphen complex as DNA hybridization marker for DNA optical biosensor to determine dengue virus.

Keywords: Dengue DNA; DNA interaction; nickel(II) salphen complex characterization; nickel(II) salfen complex

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*Corresponding author; email: nurulhuda@ukm. edu.my