Sains Malaysiana 48(10)(2019): 2221–2227

http://dx.doi.org/10.17576/jsm-2019-4810-18

 

Effect of P-Hydroxycinnamic Acid in Mice Model of Cerebral Ischemia-Reperfusion Injury

(Kesan Asid P-Hidroksisinamik dalam Model Tikus dengan Kecederaan Serebrum Iskemia-Reperfusi)

 

ROMGASE SAKAMULA, CHANIKARN SAKDAPITAK & WACHIRYAH THONG-ASA*

 

Physiology division, Animal Toxicology and Physiology, Specialty Research Unit (ATPSRU), Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand

 

Received: 3 April 2019/Accepted: 8 August 2019

 

ABSTRACT

Multiple pathomechanisms of cerebral ischemia reperfusion (I/R) injuries can be ameliorated by certain high-potential pharmaceutical substances. In the present study, we investigated the acute effect of p-hydroxycinnamic (pHCA) acid against cerebral I/R injury in mice. Thirty male ICR mice were divided into Sham, Control-I/R, and pHCA-I/R groups. The pHCA 100 mg/kg and the vehicle were given 30 min before I/R induction. Thirty-minute bilateral common carotid artery occlusion followed by 45-min reperfusion was performed on the Control-I/R and pHCA-I/R groups. Brains were collected for biochemical analysis, infarction and histological study of the cerebral cortex and corpus callosum (CC). The results showed that I/R induction significantly induced biochemical changes (p<0.05) along with the increase of brain infarction (p<0.05), percentage of degeneration in cerebral cortex (p<0.05) and decrease of CC white matter density (p<0.05). Pretreatment with pHCA significantly reduced MDA (p<0.05), brain infarction (p<0.05), cerebral cortex neuronal degeneration (p<0.05) and prevented the reduction of white matter density in the CC (p<0.05). The present study concluded that pretreatment with pHCA helps prevent cerebral I/R injury by amelioration of lipid peroxidation, white matter damage and neuronal degeneration.

Keywords: Brain ischemia; infarction; oxidative stress; p-hydroxycinnamic acid; white matter

 

ABSTRAK

Pelbagai patologi mekanisme kecederaan serebrum iskemia-reperfusi (I/R) boleh diperbaikkan oleh beberapa bahan farmaseutik berpotensi tinggi. Dalam penyelidikan ini, kami mengkaji kesan akut p-hidroksisinamik (pHCA) terhadap kecederaan I/R serebrum pada tikus. Tiga puluh tikus ICR jantan dibahagikan kepada Sham, kawalan-I/R, dan kumpulan pHCA-I/R. PHCA 100 mg/kg dan pembawa diberikan 30 min sebelum induksi I/R. Oklusi arteri karotid selama 12 min diikuti oleh reperfusi 45 min dilakukan pada kumpulan Kawalan-I/R dan pHCA-I/R. Tisu otak dikumpulkan untuk analisis biokimia, infarksi dan kajian histologi korteks serebrum dan korpus kalosum (CC). Keputusan menunjukkan bahawa induksi I/R menunjukkan perubahan biokimia yang ketara (p<0.05) dengan peningkatan infarksi otak (p<0.05), peratusan degenerasi dalam korteks serebrum (p<0.05). Pra-rawatan dengan pHCA mengurangkan MDA (p<0.05), infarksi otak (p<0.05), degenerasi neuron korteks serebrum (p<0.05) dan menghalang pengurangan kepadatan bahan putih otak di CC (p<0.05). Kajian ini menyimpulkan bahawa prarawatan dengan pHCA membantu mencegah kecederaan otak I/R dengan memperbaik peroksidasi lipid, kerosakan bahan putih otak dan degenerasi neuron.

Kata kunci: Asid p-hidroksisinamik; bahan putih otak; infarksi; iskemia otak; tekanan oksidatif

REFERENCES

Abdel-Wahab, M.H., El-Mahdy, M.A., Abd-Ellah, M.F., Helal, G.K., Khalifa, F. & Hamada, F.M. 2003. Influence of p-coumaric acid on doxorubicin-induced oxidative stress in rat’s heart. Pharmacol. Res. 48(5): 461-465.

Amalan, V., Natesan, V., Dhananjayan, I. & Arumugam, R. 2016. Antidiabetic and antihyperlipidemic activity of p-coumaric acid in diabetic rats, role of pancreatic GLUT 2: In vivo approach. Biomedicine & Pharmacotherapy 84: 230-236.

Chaitanya, G.V. & Babu, P.P. 2008. Activation of calpain, cathepsin-b and caspase-3 during transient focal cerebral ischemia in rat model. Neurochem. Res. 33(11): 2178-2186.

Elmore, S.A., Dixon, D., Hailey, J.R., Harada, T., Herbert, R.A., Maronpot, R.R., Nolte, T., Rehg, J.E., Rittinghausen, S., Rosol, T.J., Satoh, H., Vidal, J.D., Willard-Mack, C.L. & Creasy, D.M. 2016. Recommendations from the INHAND Apoptosis/Necrosis Working Group. Toxicol. Pathol. 44(2): 173-188.

Fentem, J.H. & Fry, J.R. 1992. Metabolism of coumarin by rat, gerbil and human liver microsomes. Xenobiotica 22: 357-367.

Fishbein, M.C., Meerbaum, S., Rit, J., Lando, U., Kanmatsuse, K., Mercier, J.C., Corday, E., & Ganz, W. 1981. Early phase acute myocardial infarct size quantification: Validation of the triphenyl tetrazolium chloride tissue enzyme staining technique. Am. Heart J. 101(5): 593-600.

Guven, M., Aras, A.B., Akman, T., Sen, H.M., Ozkan, A., Salis, O., Sehitoglu, I., Kalkan, Y., Silan, C., Deniz, M. & Cosar, M. 2015. Neuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia. Iran J. Basic. Med. Sci. 18(4): 356-363.

Hadwan, M.H. & Abed, H.N. 2016. Data supporting the spectrophotometric method for the estimation of catalase activity. Data in Brief 6: 194-199.

Hou, S.T. & MacManus, J.P. 2002. Molecular mechanisms of cerebral ischemia-induced neuronal death. International Review of Cytology 221: 93-148.

Kalogeris, T., Baines, C.P., Krenz, M. & Korthuis, R.J. 2012. Chapter Six - Cell biology of ischemia/reperfusion injury. International Review of Cell and Molecular Biology 298: 229-317.

Konishi, Y., Hitomi, Y. & Yoshioka, E. 2004. Intestinal absorption of p-coumaric and gallic acids in rats after oral administration. Journal of Agricultural and Food Chemistry 52: 2527-2532.

Ladecola, C. & Anrather, J. 2011. Stroke research at a crossroad: Asking the brain for directions. Nature Neuroscience 14(11): 1363-1368.

Lake, B.G. 1999. Coumarin metabolism, toxicity and carcinogenicity: Relevance for human risk assessment. Food and Chemical Toxicology 37: 423-453.

Lee, J.M., Grabb, M.C., Zipfel, G.J. & Choi, D.W. 2000. Brain tissue responses to ischemia. The Journal of Clinical Investigation 106: 723-731.

Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1): 265-275.

Luo, Y., Ma, H., Zhou, J.J., Li, L., Chen, S.R., Zhang, J., Chen, L. & Pan, H.L. 2018. Focal cerebral ischemia and reperfusion induce brain injury through α2δ-1-bound NMDA receptors. Stroke 49: 2464-2472.

Martin, L.J., Al-Abdulla, N.A., Brambrink, A.M., Kirsch, J.R., Sieber, F.E. & Portera-Cailliau, C. 1998. Neurodegeneration in excitotoxicity, global cerebral ischemia, and target deprivation: A perspective on the contributions of apoptosis and necrosis. Brain Research Bulletin 46(4): 281-309.

Mehta, S.L., Kumari, S., Mendelev, N. & Li, P.A. 2012. Selenium preserves mitochondrial function, stimulates mitochondrial biogenesis, and reduces infarct volume after focal cerebral ischemia. BMC Neurosci. 13: 79.

Mehta, S.L., Manhas, N. & Raghubir, R. 2007. Molecular targets in cerebral ischemia for developing novel therapeutics. Brain Research Reviews 54(1): 34-66.

Mendelow, A.D., Graham, D.I., McCulloch, J. & Mohamed, A.A. 1984. The distribution of ischaemic damage and cerebral blood flow after unilateral carotid occlusion and hypotension in the rat. Stroke 15(4): 704-710.

Pan, J., Konstas, A.A., Bateman, B., Ortolano, G.A. & Pile- Spellman, J. 2007. Reperfusion injury following cerebral ischemia: Pathophysiology, MR imaging, and potential therapies. Neuroradiology 49(2): 93-102.

Paxinos, G. & Franklin, K. 2008. The Mouse Brain in Stereotaxic Coordinates. 3rd ed. New York: Academic Press. p. 360.

Pei, K., Ou, J., Huang, J. & Ou, S. 2015. p-Coumaric acid and its conjugates: Dietary sources, pharmacokinetic properties and biological activities. J. Sci. Food Agric. 96(9): 2952-2962.

Phillis, J.W., O’Regan, M.H. & Donard, S.D. 2002. Energy utilization in the ischemic/reperfused brain. International Review of Neurobiology 51: 377-414.

Raghavendra, M., Rituparna, M., Shafalika, K., Anshuman, T., Sumit, M. & Acharya, S.B. 2009. Role of Centella asiatica on cerebral post-ischemic reperfusion and long-term hypoperfusion in rats. Int. J. Green Pharm. 3(2): 88-96.

Shuaib, A. & Breker-Klassen, M.M. 1997. Inhibitory mechanisms in cerebral ischemia: A brief review. Neuroscience & Biobehavioral Reviews 21(2): 219-226.

Small, D.L., Morley, P. & Buchan, A.M. 1999. Biology of ischemic cerebral cell death. Progress in Cardiovascular Diseases 42(3): 185-207.

Spare, P.D. 1964. A stable murexide reagent for the estimation of calcium in micro quantities of serum. Clinical Chemistry 10(8): 726-729.

Ueda, H. & Fujita, R. 2004. Cell death mode switch from necrosis to apoptosis in brain. Biol. Pharm. Bull. 27(7): 950-955.

Wachiryah Thong-asa & Kanokwan Tilokskulchai. 2014. Neuronal damage of the dorsal hippocampus induced by long-term right common carotid artery occlusion in rats. Iran J. Basic Med. Sci. 17(3): 220-226.

Wachiryah Thong-asa, Panas Tumkiratiwong, Vasakorn Bullangpoti, Kasem Kongnirundonsuk & Kanokwan Tilokskulchai. 2017. Tiliacora triandra (Colebr.) Diels leaf extract enhances spatial learning and learning flexibility, and prevents dentate gyrus neuronal damage induced by cerebral ischemia/reperfusion injury in mice. Avicenna Journal of Phytomedicine 7(5): 389-400.

Wachiryah Thong-asa, Kanokwan Tilokskulchai, Supin Chompoopong, Mayuree Hantrakul & Tantisira. 2015. Early onset effects of mild chronic cerebral hypoperfusion on the dorsal hippocampus and white matter areas: The use of male Sprague-Dawley rats as a UCO model. Journal of Neurological Sciences 32(1): 030-039.

Wakita, H., Tomimoto, H., Akiguchi, I., Matsuo, A., Lin, J.X., Ihara, M. & McGeer, P.L. 2002. Axonal damage and demyelination in the white matter after chronic cerebral hypoperfusion in the rat. Brain Res. 924(1): 63-70.

Wang, Y., Liu, G., Hong, D., Chen, F., Ji, X. & Cao, G. 2016. White matter injury in ischemic stroke. Prog. Neurobiol. 141: 45-60.

White, B.C., Sullivan, J.M., DeGracia, D.J., O’ Neil, B.J., Neumar, R.W., Grossman, L.I., Rafols, J.A. & Krause, G.S. 2000. Brain ischemia and reperfusion: Molecular mechanisms of neuronal injury. Journal of the Neurological Sciences 179: 1-33.

Yoon, J.H., Youn, K., Ho, C.T., Karwe, M.V., Jeong, W.S. & Jun, M. 2014. p-Coumaric acid and ursolic acid from Corni fructus attenuated beta-amyloid (25-35)-induced toxicity through regulation of the NF-kappaB signaling pathway in PC12 cells. J. Agric. Food Chem. 62(21): 4911-4916.

 

*Corresponding author; email: fsciwyth@ku.ac.th

 

 

 

 

 

previous