Sains Malaysiana 48(4)(2019): 813–822

http://dx.doi.org/10.17576/jsm-2019-4804-13

 

Lead Accumulation and Its Histological Impact on Cymodocea serrulata Seagrass in the Laboratory

(Pengumpulan Plumbum dan Kesan Histologinya pada Rumpai Laut Cymodocea serrulata dalam Makmal)

DWI ROSALINA¹*, ENDANG YULI HERAWATI¹, MUHAMMAD MUSA¹, DINI SOFARINI²,

MOHAMMAD AMIN³ & YENNY RISJANI¹

 

¹Water Resources Management, Faculty of Fisheries and Marine Sciences, University of Brawijaya Malang, Malang 65145, Indonesia

 

²Water Resources Management, Faculty of Fisheries and Marine Sciences, University of Lambung Mangkurat, South Kalimantan, Indonesia

 

³Biology, Faculty of Mathematic and Sciences, University of Malang, Malang 65145, Indonesia

 

Received: 14 November 2018/Accepted: 17 February 2019

 

ABSTRACT

The purpose of this study was to determine the concentration of lead (Pb) in Cymodocea serrulata tissues (roots, rhizomes, and leaves) using the AAS method, also to figure out Pb’s impact on seagrass’ histology and elements using the SEM-EDX Mapping method. The results showed that the higher the concentration and the length of the planting period, the higher the accumulation of heavy metals in the seagrass tissues. In this study, Pb was largely accumulated in the leaves, roots, and rhizomes tissue. Moreover, the seagrass histology in the epidermis and endodermis underwent shape and structure changes; it also went through damage or thickening at 15 ppm concentration, compared to control. This study strengthens the usefulness and relationship of Cymodocea serrulata seagrass as a biological indicator of metal contamination in the waters.

 

Keywords: Cymodocea serrulata; lead; mapping; seagrass; SEM-EDX

 

ABSTRAK

Tujuan kajian ini adalah untuk menentukan kepekatan plumbum (Pb) di dalam tisu Cymodocea serrulata (akar, rizom dan daun) menggunakan kaedah AAS, serta mengkaji kesan Pb pada histologi dan unsur rumpai laut menggunakan kaedah pemetaan SEM-EDX. Keputusan menunjukkan bahawa semakin tinggi kepekatan dan tempoh penanaman yang lama, semakin tinggi pengumpulan logam berat dalam tisu rumpai laut. Dalam kajian ini, Pb banyak terkumpul di bahagian tisu daun, akar dan rizom. Selain itu, histologi rumpai laut di epidermis dan endodermis mengalami perubahan bentuk dan struktur; ia juga mengalami kerosakan atau penebalan pada kepekatan 15 ppm, berbanding dengan kawalan. Kajian ini mengukuhkan lagi kegunaan rumpai laut jenis Cymodocea serrulata sebagai penanda biologi untuk mengesan pencemaran logam dalam laut.

 

Kata kunci: Cymodocea serrulata; pemetaan; plumbum; rumpai laut; SEM-EDX

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*Corresponding author; email: myrafirifky@gmail.com