Sains Malaysiana 48(9)(2019): 1989–1995

http://dx.doi.org/10.17576/jsm-2019-4809-20

Effect of Tiliacora triandra Leaf Extract on Glycemic Control in Mice with High Sugar Intake

(Kesan Ekstrak Daun Tiliacora triandra ke atas Kawalan Glisemik pada Tikus dengan Pengambilan Gula yang Tinggi)

WACHIRYAH THONG-ASA¹*, PRACHAYAPORN PRASERTSUKSRI², ROMGASE SAKAMULA¹ & THEERAYUT NIMNUAN¹

¹Animal Toxicology and Physiology Specialty Research Unit (ATPSRU), Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand

²Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand

Received: 4 April 2019/Accepted: 11 June 2019

ABSTRACT

Prediabetes is associated with sugar-sweetened beverages and sugar-rich processed foods. High sugar intake (HSI), which is represented as save dose, may silently alter the glycemic control. The present study investigated the effect of HSI and Tiliacora triandra (TT21) leaf extract on the glycemic control in mice. Eighteen male ICR mice were divided into three groups of Control-HSI, HSI-TT300 and HSI-TT600 that received 4 weeks of 30% glucose with a vehicle of T. triandra leaf extract 300 and 600 mg/kg, respectively. The blood glucose, serum insulin, glucose clearance, liver and muscle glycogen contents and tissue oxidative status were evaluated. The results showed that HSI increased the blood glucose (w2 and w3: p<0.05) and serum insulin levels (w3 and w4: p<0.05) with glucose intolerance (w4: 30, 60 and 90 min, p<0.05). T. triandra leaf extract reduced the blood glucose and serum insulin and increased the glycogen content in the liver and muscle tissues (p<0.05). We concluded that HSI silently induced an alteration of the glycemic control in the normal mice, and the T. triandra leaf extract nurtured the glycemic control in the HSI mice by lowering the blood glucose and serum insulin and increasing the liver and muscle glycogen contents, which indicated an involvement of peripheral insulin sensitivity.

Keywords: Glucose clearance; glucose metabolism; glycemic control; high sugar intake; Tiliacora triandra

ABSTRAK

Pra-diabetes telah dikaitkan dengan pengambilan minuman manis dan makanan yang kaya dengan gula. Pengambilan gula tinggi (HSI) yang dikenali sebagai dos simpanan mungkin secara perlahan mengubah kawalan glisemik. Penyelidikan ini mengkaji kesan HSI dan ekstrak daun Tiliacora triandra (TT21) terhadap kawalan glisemik pada tikus. Lapan belas ekor tikus ICR jantan telah dibahagikan kepada 3 kumpulan, iaitu kawalan-HSI, HSI-TT300 dan HSI-TT600 yang menerima 30% glukosa selama 4 minggu dengan ekstrak daun T. triandra 300 dan 600 mg/kg sebagai pembawa. Glukosa darah, serum insulin, pelepasan glukosa, kandungan glikogen pada hepar dan otot serta status oksidatif tisu telah dinilai. Hasil kajian menunjukkan bahawa HSI meningkatkan glukosa darah (w2 dan w3: p<0.05) dan tahap insulin serum (w3 dan w4: p<0.05) dengan intoleransi glukosa (w4: 30, 60 dan 90 min, p<0.05). Ekstrak daun T. triandra mengurangkan glukosa darah, insulin serum dan peningkatan kandungan glikogen dalam tisu hepar dan otot (p<0.05). Berdasarkan keputusan kajian, kesimpulan bahawa HSI secara perlahan mendorong perubahan kawalan glisemik pada tikus normal dan ekstrak daun T. triandra mengawal kawalan glisemik dalam tikus HSI dengan menurunkan glukosa darah, insulin serum dan peningkatan kandungan glikogen pada hepar dan otot yang menunjukkan penglibatan kepekaan insulin periferi.

Kata kunci: Kawalan glisemik; metabolisme glukosa; pelepasan glukosa; pengambilan gula yang tinggi; Tiliacora triandra

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*Corresponding author; email: fsciwyth@ku.ac.th