Sains Malaysiana 49(1)(2020): 103-112

http://dx.doi.org/10.17576/jsm-2020-4901-12

 

Effect of Purified Soluble Polysaccharides Extracted from Gray Oyster Mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 Adipocytes

(Kesan Polisakarida Terlarut Tulen Diekstrak daripada Cendawan Tiram [Pleurotus sajor-caju (Fr.) Sing.] pada Adiposit 3t3-L1)

 

DECHA SERMWITTAYAWONG1*, KULWANIT PATNINAN1, CHANAWEE JAKKAWANPITAK1, SOMRUTHAI PHOTHIPHIPHIT2, SIWARUTT BOONYARATTANAKALIN2, MASASHI INAFUKU3, HIROSUKE OKU3, KUSUMARN NOIPHA4 & NONGPORN HUTADILOK-TOWATANA5

 

1Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand

 

2School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani 12121, Thailand

 

3Molecular Biotechnology Group, Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan

 

4Faculty of Health and Sports Science, Thaksin University, Paphayom, Phatthalung, 93110, Thailand

 

5Krisana Kraisintu Foundation, 13/217 Plum Condominiums Phaholyothi, Thailand

 

Received: 24 May 2019/Accepted: 15 October 2019

 

ABSTRACT

Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years.  The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In this study, we used hot water and ammonium oxalate to extract polysaccharides from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] and subsequently purified with enzymatic digestion, solvent extraction and ion-exchange chromatography. One of the fractions, 7S1-1, has the highest yield and purity and contains polysaccharides with the molecular weight around 500 kDa. In addition, Fourier transformed infrared spectroscopy (FTIR) and monosaccharide composition analyses showed that this 7S1-1 sample contains mostly B-glucan and some mannan. We find that the 7S1-1 fraction does not affect the total amount of triglycerides in the 3T3-L1 adipocytes but promotes a release of glycerol of the fat cells, suggesting that the fraction does not inhibit adipogenesis but stimulates lipolysis. Laminarin, a small B-glucan, confers similar results to the 7S1-1 sample, but it could not stimulate lipolysis as well as the 7S1-1 sample. Therefore, these results suggest a potential anti-obesity activity of the mushroom polysaccharides.

 

Keywords: Anti-obesity; lipolysis; Pleurotus sajor-caju; polysaccharides; 3T3-L1 adipocytes

 

ABSTRAK

Cendawan fungsian polisakarida telah digunakan dalam perubatan tradisi di Asia selama bertahun-tahun. Aktiviti anti-obesiti cendawan polisakarida telah ditunjukkan secara in vivo dan in vitro. Walau bagaimanapun, kesan polisakarida tulen pada pencilan sel lemak belum pernah ditunjukkan. Dalam kajian ini, kami menggunakan air panas dan ammonium oksalat untuk mengekstrak polisakarida daripada cendawan tiram [Pleurotus sajor-caju (Fr.) Sing.]  dan kemudiannya ditulenkan dengan penghadaman enzim, pengekstrakan pelarut dan kromatografi pertukaran ion. Salah satu daripada pecahan, 7S1-1, mempunyai hasil dan penulenan tertinggi serta mengandungi polisakarida dengan berat molekul sekitar 500 kDa. Di samping itu, Spektroskopi transformasi Fourier inframerah (FTIR) dan analisis monosakarida menunjukkan bahawa sampel 7S1-1 ini mengandungi B-glukan kebanyakannya dan sedikit mannan. Kami mendapati bahawa pecahan 7S1-1 tidak memberi kesan kepada jumlah trigliserida dalam adiposit 3T3-L1 tetapi menggalakkan pembebasan gliserol daripada sel lemak, justeru mencadangkan bahawa pecahan tidak menghalang adipogenesis tetapi merangsang lipolisis. Laminarin, B-glukan kecil menghasilkan keputusan yang sama kepada sampel 7S1-1, tetapi ia tidak boleh merangsang lipolisis sebaik sampel 7S1-1. Oleh itu, keputusan ini mencadangkan aktiviti anti-obesiti yang berpotensi daripada cendawan polisakarida.

 

Kata kunci: Adiposit 3T3-L1;  anti-obesiti; lipolisis; Pleurotus sajor-caju; polisakarida

 

REFERENCES

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1): 248-254. doi:https://doi.org/10.1016/0003-2697(76)90527-3.

Chen, J.Z. & Seviour, R. 2007. Medicinal importance of fungal β-(1→3), (1→6)-glucans. Mycological Research 111(6): 635-652. doi:10.1016/j.mycres.2007.02.011.

Dubois, M., Gilles, K., Hamilton, J.K., Rebers, P.A. & Smith, F. 1951. A colorimetric method for the determination of sugars. Nature 168(4265): 167.

Folch, J., Lees, M. & Sloane, S.G.H. 1957. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226(1): 497-509.

Galichet, A. 2001. FTIR spectroscopic analysis of saccharomyces cerevisiae cell walls: Study of an anomalous strain exhibiting a pink-colored cell phenotype. FEMS Microbiology Letters 197(2): 179-186. doi:10.1016/S0378-1097(01)00101-X.

Hu, J.L., Nie, S.P., Wu, Q.M., Li, C., Fu, Z.H., Gong, J., Cui, S.W. & Xie, M.Y. 2014. Polysaccharide from seeds of Plantago asiatica L. affects lipid metabolism and colon microbiota of mouse. J. Agric. Food Chem. 62(1): 229-234. doi:10.1021/jf4040942.

Jantaramanant, P., Sermwittayawong, D., Noipha, K., Hutadilok-Towatana, N. & Wititsuwannakul, R. 2014. β-glucan-containing polysaccharide extract from the grey oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] stimulates glucose uptake by the L6 myotubes. International Food Research Journal 21(2): 779-784.

Kanagasabapathy, G., Chua, K.H., Malek, S.N.A., Vikineswary, S. & Kuppusamy, U.R. 2014. AMP-activated protein kinase mediates insulin-like and lipo-mobilising effects of β-glucan-rich polysaccharides isolated from Pleurotus sajor-caju (Fr.), singer mushroom, in 3T3-L1 cells. Food Chemistry 145: 198-204. doi:10.1016/j.foodchem.2013.08.051.

Kanagasabapathy, G., Malek, S.N., Mahmood, A.A., Chua, K.H., Vikineswary, S. & Kuppusamy, U.R. 2013. Beta-glucan-rich extract from Pleurotus sajor-caju (Fr.) singer prevents obesity and oxidative stress in C57BL/6J mice fed on a high-fat diet. Evid. Based Complement. Alternat. Med. 2013: 185259. doi:10.1155/2013/185259.

Kanagasabapathy, G., Kuppusamy, U.R., Abd Malek, S.N., Abdulla, M.A., Chua, K.H. & Sabaratnam, V. 2012. Glucan-rich polysaccharides from Pleurotus sajor-caju (Fr.) singer prevents glucose intolerance, insulin resistance and inflammation in C57BL/6J mice fed a high-fat diet. BMC Complement. Altern. Med. 12: 261. doi:10.1186/1472-6882-12-261.

Sermwittayawong, D., Patninan, K., Phothiphiphit, S., Boonyarattanakalin, S., Sermwittayawong, N. & Hutadilok-Towatana, N. 2018. Purification, characterization, and biological activities of purified polysaccharides extracted from the gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.]. Journal of Food Biochemistry 42(5): e12606. doi:10.1111/jfbc.12606.

Sun, W.M., Wang, Y.P., Duan, Y.Q., Shang, H.X. & Cheng, W.D. 2014. Radix hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of nonalcoholic fatty liver disease via adenosine monophosphateactivated protein kinase pathway activation. Mol. Med. Rep. 10(3): 1237-1244. doi:10.3892/mmr.2014.2327.

Naoyuki Taira, Ruwani N Nugara, Masashi Inafuku, Kensaku Takara, Takayuki Ogi, Toshio Ichiba, Hironori Iwasaki, Takafumi Okabe. & Hirosuke Oku. 2017. In vivo and in vitro anti-obesity activities of dihydropyranocoumarins derivatives from Peucedanum japonicum Thunb. Journal of Functional Foods 29: 19-28. doi:https://doi.org/10.1016/j.jff.2016.11.030.

Wang, K.P., Cao, P., Shui, W.Z., Yang, Q.X., Tang, Z.H. & Yu, Z. 2015. Angelica sinensis polysaccharide regulates glucose and lipid metabolism disorder in prediabetic and streptozotocin-induced diabetic mice through the elevation of glycogen levels and reduction of inflammatory factors. Food & Function 6(3): 902-909. doi:10.1039/C4FO00859F.

Wang, X., Liu, H., Zhao, L., Dong, H., Wang, Y., Sun, D., Liu, Y., Yang, R. & Li, X. 2012. Effects of crude polysaccharides from Catathelasma ventricosum on the proliferation and differentiation of 3T3-L1 cells. Agricultural Journal 7(3): 187-190.

 

*Corresponding author; email: decha.s@psu.ac.th

   

 

 

 

 

previous