Sains Malaysiana 50(11)(2021): 3241-3250

http://doi.org/10.17576/jsm-2021-5011-08

Separation of Polar Compounds using Poly(Ethylene Oxide) Bonded Stationary Capillary Liquid Chromatography

(Pengasingan Sebatian Kutub menggunakan Kromatografi Cecair Kapilari Terikat Poli(Etilena Oksida))

ROZA LINDA¹*, ABDULLAH¹, MOHAMAD RAFI², ASTER RAHAYU³, LEE WAH LIM⁴ & TOYOHIDE TAKEUCHI⁴

¹Department of Chemistry Education, Faculty of Education and Teacher Training, University of Riau, Pekanbaru, Indonesia

²Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia

³Department of Chemical Engineering, Faculty of Industrial Technology, Ahmad Dahlan University, Yogyakarta, Indonesia

⁴Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan

Received: 21 April 2020/Accepted: 15 March 2021

ABSTRACT

Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH₂-60, 5 µm particle size, and 60 Å mean pore diameter). The prepared stationary phase was able to separate polar compounds such as phenolics and nucleobases in capillary liquid chromatography. The retention and separation of phenolics and nucleobases could be achieved under isocratic elution condition. Nucleobases such as thymine, adenine, uracil, uridine, cytidine and toluene and phenolics (phenol, pyrocatechol, pyrogallol) were baseline separated in less than 6 min using 98% acetonitrile and less than 7 minutes using 80% acetonitrile, respectively. We demonstrated that the retention of nucleobases as analyte decreased with decreasing eluent concentration. The retention of these polar compounds was believed to be based on dipole-dipole and/or hydrogen-bonding interactions.

Keywords: Capillary liquid chromatography; poly(ethylene oxide); polar compounds

ABSTRAK

Fasa pegun terikat poli(etilena oksida) (PEO) telah disintesis melalui tindak balas langkah tunggal dan sederhana. Poli(etilena glikol monometil eter p-toluena sulfonat) (tosilated-PEO, berat molekul 900, n ≈ 18) secara kimia terikat pada silika 3-aminopropil (TSKgel NH₂-60, ukuran zarah 5 µm dan diameter liang 60%). Fasa pegun yang disediakan dapat memisahkan sebatian kutub seperti fenol dan nukleobes dalam kromatografi cecair kapilari. Pengekalan dan pemisahan fenol dan nukleobes dapat dicapai dalam keadaan elusi isokratik. Nukleobes seperti timin, adenin, urasil, uridin, sitidin dan toluena serta fenol (fenol, pirokatekol, pirogalol) dipisahkan awal dalam masa kurang dari 6 minit menggunakan 98% asetonitril dan kurang dari 7 minit masing-masing menggunakan 80% asetonitril. Kami menunjukkan bahawa pengekalan nukleobes sebagai analit menurun dengan penurunan kepekatan eluen. Pengekalan sebatian kutub ini dipercayai berdasarkan interaksi ikatan dipol-dipol dan/atau hidrogen.

Kata kunci: Kromatografi cecair kapilari; polietilena oksida; sebatian kutub  

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*Corresponding author; email: rozalinda@gmail.com