Sains Malaysiana 50(5)(2021): 1243-1254

http://doi.org/10.17576/jsm-2021-5005-05

Kesignifikan Sistematik Ciri Anatomi Pina dan Stip Angiopteris angustifolia C. Presl dan A. evecta (G. Forst.)  Hoffm. [Marattiaceae] di Semenanjung Malaysia

(Systematic Significance of Anatomical Characteristics of Pinna and Stipe in Angiopteris angustifolia C. Presl and A. evecta (G. Forst.) Hoffm. [Marattiaceae] in Peninsular Malaysia)

NORAINI TALIP¹*, SITI NOR AZAH AYOB², HAJA MAIDEEN KADER MAIDEEN¹, HAMIDUN BUNAWAN³ & MOHAMAD RUZI ABDUL RAHMAN¹

¹School of Biological Sciences & Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

²Jabatan Pertanian, Bahagian Biosekuriti Tumbuhan, Wisma Tani, Jalan Sultan Salahuddin, 50632 Kuala Lumpur, Wilayah Persekutuan, Malaysia

³Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 29 May 2020/Accepted: 6 October 2020

ABSTRAK

Kajian perbandingan ciri anatomi dan ciri mikromorfologi pina dan stip Angiopteris angustifolia C. Presl dan A. evecta (G. Forst.) Hoffm. [Marattiaceae] di Semenanjung Malaysia telah dijalankan. Sampel kajian diperoleh dari Bangi, Selangor, Bukit Fraser dan Tanah Tinggi Cameron, Pahang. Objektif kajian adalah untuk mengenal pasti dan menyenaraikan ciri anatomi dan mikromorfologi pina yang boleh digunakan untuk pengecaman dan pembezaan dua spesies Angiopteris. Ciri yang ditemui boleh digunakan untuk membina kekunci pengecaman bagi kedua-dua spesies yang dikaji. Anatomi pina dan stip diperhatikan melalui kaedah hirisan dengan mikrotom gelongsor, siatan epidermis dan penjernihan serta cerapan di bawah mikroskop cahaya menggunakan perisian Cell B. Kajian mikromorfologi pula melibatkan teknik saduran emas, titik pengeringan kritikal dan cerapan di bawah mikroskop imbasan elektron menggunakan perisian SmartSEM. Ciri sepunya bagi kedua-dua spesies adalah kehadiran stel ringkas bentuk arka pada stip dan tulang pina, kehadiran sel musilaj yang agak padat pada sel parenkima korteks, peruratan tengah pina dikotom, peruratan tepi pina terbuka dan tidak bercabang dan kehadiran trikom berlengan pada tulang pina. Ciri diagnosis yang ditemui ialah peruratan palsu pada pina A. angustifolia, bentuk luaran permukaan abaksial stip A. angustifolia berbentuk bulatan lebar manakala A. evecta berbentuk ¾ bulatan. Hiasan kutikel pada epidermis abaksial pina A. angustifolia dengan dinding periklin tenggelam dan dinding antiklin timbul sebaliknya pada A. evecta dinding periklin timbul dan dinding antiklin tenggelam. Kesimpulannya, ciri anatomi dan mikromorfologi pina dan stip mempunyai nilai taksonomi yang boleh digunakan bagi pengecaman dan pembezaan kedua-dua spesies Angiopteris yang dikaji.

Kata kunci: Anatomi; Angiopteris angustifolia; Angiopteris evecta; Marattiaceae; pina

ABSTRACT

A comparative study on the pinna and stipe anatomical and micromorphological characters of Angiopteris angustifolia C. Presl and A. evecta (G. Forst.) Hoffm. [Marattiaceae] in Peninsular Malaysia has been conducted. Fresh samples were collected from Bangi, Selangor, Fraser Hill and Cameron Highland, Pahang. The objectives of the study were to determine and to identify the pinna anatomical and micromorphological characteristics that could be useful to differentiate two Angiopteris species. These characters could be used to construct a dichotomy key for the identification of the species. The anatomy of pinna and stipe were observed through sectioning of sliding microtome, pinna leaf clearing and pinna epidermis mechanical scrapping, and observation under light microscope using Cell B software. For micromorphological study, the techniques involved were gold coating, critical point drying and observation under electron scanning microscope using SmartSEM software. General characteristics in both species were presence of simple stele with arch-shaped in the stipe and midribs, presence of mucilage cells which was relatively dense in the cortical parenchyma cells, dichotomous areolar venation in pinna, an open and unbranched marginal venation system and the presence of branched trichomes in the midribs. The diagnostic characteristics found were pinna false vein in A. angustifolia, a wide circular shape of abaxial stipe surface of  A. angustifolia whereas ¾ circular in A. evecta. The cuticle ornamentation on abaxial epidermal pinna in A. angustifolia showed sunken periclinal wall, and raised anticlinal wall, whilst raised periclinal wall and sunken anticlinal wall in A. evecta. As a conclusion, the anatomical and micromorphological characteristics of pinna and stipe have taxonomic value which can be used to identify and to differentiate both Angiopteris species studied.

Keywords: Anatomy; Angiopteris angustifolia; Angiopteris evecta; Marattiaceae; pinna

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*Corresponding author; email: ntalip@ukm.edu.my