Sains Malaysiana 50(8)(2021): 2229-2240

http://doi.org/10.17576/jsm-2021-5008-08

Viability of Lactobacillus plantarum TISTR 2083 in Protectant during Low-Temperature Drying and Storage

(Kelangsungan Lactobacillus plantarum TISTR 2083 dalam Pelindung semasa Pengeringan pada Suhu Rendah dan Penyimpanan)

ABDULLAH AL MAMUN¹, PAYAP MASNIYOM² & JARUWAN MANEESRI¹*

¹Department of Food Science and Nutrition, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand

²Department of Technology and Industry, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand

Received: 25 August 2020/Accepted: 20 December 2020

ABSTRACT

Low-temperature drying was applied as simple and low cost drying technique for the production of dried Lactobacillus plantarum TISTR 2083, which was isolated from traditional starter of Ka-nom Tuay-fu. To improve the survival of L. plantarum TISTR 2083 during drying and storage, five different protectants and two carrier materials were investigated. These include sucrose, trehalose, maltodextrin, skim milk, and L-glutamate sodium salt (L-GSS) as protectant and rice starch and rice flour as carrier material. Whereas, skim milk as protectant with rice starch resulted in higher viable cell (8.71 log CFU/g) after drying by hot air oven at 40 °C. Different concentration 5, 10, and 15% (w/v) skim milk also investigated to check the effect of skim milk concentration on cell viability. L. plantarum TISTR 2083 starter powder was in different storage conditions to check the storage stability. After 90 days of storage, starter powder stored at 4 °C with silica gel and without silica gel had more than 80% survival rate, while there was no viable cell that stored in room temperature with silica gel. The result showed the production and storage conditions of high viability L. plantarum TISTR 2083, which can be used as starter culture for further fermented product development and as probiotic.

Keywords: Lactobacillus plantarum; low-temperature drying; protective agent; starter culture

ABSTRAK

Pengeringan suhu rendah digunakan sebagai teknik pengeringan yang mudah dan melibatkan kos yang rendah untuk penghasilanLactobacillus plantarum TISTR 2083 jenis kering, yang dipencil daripada pemula tradisi Ka-nom Tuay-fu. Sehubungan dengan itu, bagi mempertingkat ketahanan selL. plantarum TISTR 2083 semasa pengeringan dan penyimpanan, lima bahan pelindung dan dua bahan pembawa telah digunakan dalam uji kaji ini. Ini termasuk sukrosa, trehalosa, maltodekstrin, susu skim dan L-GSS sebagai pelindung. Pati beras dan tepung beras digunakan sebagai bahan pembawa. Manakala, susu skim yang digunakan sebagai bahan pelindung dengan pati beras menghasilkan sel yang lebih baik (8.71 log CFU/g) setelah dikeringkan dengan ketuhar udara panas pada suhu 40 °C. Kepekatan susu skim yang berbeza 5, 10 dan 15% (w/v) diuji untuk memeriksa kesan kepekatan susu skim terhadap ketahanan sel. Serbuk pemula L. plantarum TISTR 2083 berada dalam keadaan penyimpanan yang berbeza untuk memeriksa kestabilan penyimpanan. Setelah 90 hari penyimpanan, serbuk pemula yang disimpan pada suhu 4 °C, dengan gel silika dan tanpa gel silika mempunyai kadar kelangsungan hidup lebih daripada 80%, sementara tiada sel yang dapat digunakan apabila disimpan dalam gel silika pada suhu bilik. Hasil kajian menunjukkan penghasilan dan keadaan penyimpanan berketahanan tinggiL. plantarum TISTR 2083, boleh digunakan sebagai kultur pemula fermentasi pada masa hadapan dan sebagai probiotik.

Kata kunci: Agen pelindung; kultur pemula: Lactobacillus plantarum; pengeringan suhu rendah

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*Corresponding author; email: jaruwan.ma@psu.ac.th