Sains Malaysiana 51(4)(2022): 1099-1109

http://doi.org/10.17576/jsm-2022-5104-12

 

Optimum Extraction Condition of Anacardium occidentale on Antiurolithiatic Activities (in-vitro)

(Keadaan Pengekstrakan Optimum Anacardium occidentale pada

Aktiviti Antiurolithiatik (in-vitro))

 

NUR FAZIRA ABDUL RAHIM, NORHAYATI MUHAMMAD* & NORAZLIN ABDULLAH

 

Department of Technology and Natural Resources, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, UTHM - Campus (Pagoh Branch), Pagoh Education Hub, KM 1, Jalan Panchor, 84600 Muar, Johor Darul Takzim, Malaysia

 

Received: 9 June 2021/Accepted: 1 September 2021

 

ABSTRACT

Urolithiasis or kidney stone has become a worldwide problem including Malaysia. Its management is depending on surgical procedures that are quite costly and can cause reoccurrence. Anacardium occidentale (cashew) has been used traditionally to treat urolithiasis but no scientific data has been recorded that proven its efficiency particularly on its extraction parameters. Therefore, this study aims to identify the optimum extraction parameters of A. occidentale leaves extracts on in-vitro antiurolithiatic activities using Response Surface Methodology (RSM). The effect of three extraction parameters (solvent concentration, X1; extraction temperature, X2; extraction time, X3) on antiurolithiatic activities (turbidity or nucleation assay, Y1; titrimetric or calcium oxalate dissolution assay, Y2) were determined by Central Composite Design (CCD). The results showed that each parameter and its interaction significantly affect the antiurolithiatic activities (p < 0.05). The quadratic model was closely fitted for all responses as the R2 values obtained were 0.9786 (turbidity assay) and 0.9956 (titrimetric assay). The optimum extraction condition of A. occidentale leaves extract suggested by the predicted model was at 0.4% ethanol concentration, temperature 31.5 and for 30 min of extraction time, exhibited antiurolithiatic activities of 85.57 ± 0.43% (turbidity) and 96.48 ± 0.70% (titrimetric). The phytochemical screening was done on the presence of phenols, tannins, flavonoids, alkaloids, saponins as well as terpernoids and all phytochemicals were identified in the optimized A. occidentale leaves extract. This study has given basic scientific evidence that optimum extraction conditions are necessary to obtain optimum antiurolithiatic activity.

 

Keywords: Anacardium occidentale; antiurolithiatic; extraction parameters; in-vitro; RSM

 

ABSTRAK

Urolithiasis atau batu karang telah menjadi permasalahan di seluruh dunia termasuk Malaysia. Pengurusannya bergantung pada prosedur pembedahan yang agak mahal dan boleh menyebabkan pembentukan semula. Anacardium occidentale (gajus) telah digunakan secara tradisi untuk merawat urolitiatik tetapi tiada data saintifik yang direkodkan bagi membuktikan keberkesanannya terutama pada parameter pengekstrakannya. Oleh itu, kajian ini bertujuan untuk mengenal pasti parameter pengekstrakan yang optimum bagi ekstrak daun A. occidentale secara in-vitro terhadap aktiviti antiurolitiatik melalui Kaedah Permukaan Tindak Balas (RSM). Kesan tiga parameter pengekstrakan (kepekatan pelarut, X1; suhu pengekstrakan, X2; masa pengekstrakan, X3) terhadap aktiviti antiurolitiatik (kaedah kekeruhan atau nukleasi, Y1; kaedah titrimetrik atau pembubaran kalsium oksalat, Y2) ditentukan oleh Pusat Reka Bentuk Komposit (CCD). Hasil kajian menunjukkan bahawa setiap parameter dan interaksinya secara signifikan mempengaruhi aktiviti antiurolitiatik (p < 0.05). Model kuadratik telah dipilih untuk semua respons kerana nilai R2 yang diperoleh adalah 0.9786 (kaedah kekeruhan) dan 0.9956 (kaedah titrimetrik). Keadaan pengekstrakan optimum ekstrak daun A. occidentale yang dicadangkan oleh model ramalan adalah pada kepekatan etanol 0.4%, suhu 31.5 °C dan 30 min masa pengekstrakan, menunjukkan aktiviti antiurolitiatik 85.57 ± 0.43% (kekeruhan) dan 96.48 ± 0.70% (titrimetrik). Ujian fitokimia dilakukan bagi menentukan kehadiran fenol, tanin, flavonoid, alkaloid, saponin serta terpernoid dan kesemua fitokimia telah dapat dikenal pasti dalam ekstrak daun A. occidentale yang dioptimumkan. Kajian ini telah memberikan bukti saintifik asas bahawa keadaan pengekstrakan optimum diperlukan untuk menghasilkan aktiviti antiurolitiatik yang optimum.

 

Kata kunci: Anacardium occidentale; antiurolitiatik; in-vitro; parameter pengekstrakan; RSM

 

References

Abu Bakar, F.I., Abu Bakar, M.F., Abdullah, N., Endrini, S. & Fatmawati, S. 2020. Optimization of extraction conditions of phytochemical compounds and anti-gout activity of Euphorbia hirta L. (ara tanah) using response surface methodology and liquid chromatography-mass spectrometry (LC-MS) analysis. Evidence-Based Complementary and Alternative Medicine 2020: Article ID. 4501261.

Abu-reidah, I.M., Ali-shtayeh, M.S., Jamous, R.M., Arráez-román, D. & Segura-carretero, A. 2015. HPLC–DAD–ESI-MS/MS screening of bioactive components from Rhus coriaria L. (Sumac) fruits. Food Chemistry 166: 179-191.

Alara, O.R., Abdurahman, N.H. & Olalere, O.A. 2020. Ethanolic extraction of flavonoids, phenolics and antioxidants from Vernonia amygdalina leaf using two-level factorial design. Journal of King Saud University - Science 32(1): 7-16.

Ankur, C., Amarchand, P., Aadarsh, C., Deepa, I., Pawar, R. & Patil, U. 2010. Potential of medicinal plants in kidney, gall and urinary stones. International Journal of Drug Development & Research 2(2): 431-447.

Atodariya, U., Barad, R., Upadhyay, S. & Upadhyay, U. 2013. Anti-urolithiatic activity of Dolichos biflorus seeds. Journal of Pharmacognosy and Phytochemistry 2(2): 209-213.

Bahmani, M., Baharvand-Ahmadi, B., Tajeddini, P., Rafieian-Kopaei, M. & Naghdi, N. 2016. Identification of medicinal plants for the treatment of kidney and urinary stones. Journal of Renal Injury Prevention 5(3): 129-133.

Banerjee, S. & Chatterjee, J. 2015. Efficient extraction strategies of tea (Camellia sinensis) biomolecules. Journal of Food Science and Technology 52(6) 3158-3168.

Behera, S.K., Meena, H., Chakraborty, S. & Meikap, B.C. 2018. Application of response surface methodology (RSM) for optimization of leaching parameters for ash reduction from low-grade coal. International Journal of Mining Science and Technology 28(4): 621-629.

Belwal, T., Dhyani, P., Bhatt, I.D., Rawal, R.S. & Pande, V. 2016. Optimization extraction conditions for improving phenolic content and antioxidant activity in Berberis asiatica fruits using response surface methodology (RSM). Food Chemistry 207: 115-124.

Benalia, H., Djeridane, A., Bensafieddine, F. & Yousfi, M. 2016. High in vitro antiurolithiatic effect of Pituranthos scoparius roots extracts. Pharmacologyonline 1: 31-43.

Chan, E.W.C., Baba, S., Chan, H.T., Kainuma, M., Inoue, T. & Wong, S.K. 2017. Ulam herbs: A review on the medicinal properties of Anacardium occidentale and Barringtonia racemosa. Journal of Applied Pharmaceutical Science 7(2): 241-247.

Che Sulaiman, I.S., Basri, M., Fard Masoumi, H.R., Chee, W.J., Ashari, S.E. & Ismail, M. 2017. Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chemistry Central Journal 11(1): 1-11.

Dibazar, R., Bonat Celli, G., Brooks, M.S.L. & Ghanem, A. 2015. Optimization of ultrasound-assisted extraction of anthocyanins from lowbush blueberries (Vaccinium angustifolium Aiton). Journal of Berry Research 5(3): 173-181.

He, Q., Du, B. & Xu, B. 2018. Extraction optimization of phenolics and antioxidants from black goji berry by accelerated solvent extractor using response surface methodology. Applied Sciences 8(10): 1905.

Hussein, N., Sadiq, S., Kamaliah, M., NorAkmal, A. & Gohar, M. 2013. Twenty-four-hour urine constituents in stone formers: a study from the northeast part of Peninsular Malaysia. Saudi Journal of Kidney Diseases and Transplantation 24(3): 630-637.

Ilaiyaraja, N., Likhith, K.R., Sharath Babu, G.R. & Khanum, F. 2015. Optimisation of extraction of bioactive compounds from Feronia limonia (wood apple) fruit using response surface methodology (RSM). Food Chemistry 173: 348-354.

Kamaludin, N.H.I. & Jaafar, N.S.A. 2017. Evaluation of antioxidant activity from different plant parts of senduduk herb: Extraction conditions optimization of selected plant part. MATEC Web of Conferences 97: 01101.

Khare, P., Mishra, V.K., Arun, K., Bais, N. & Singh, R. 2014. Study on in-vitro antilithiatic activity of Phyllanthus niruri Linn. leaves by homogenous precipitation and turbiditory method. International Journal of Pharmacy and Pharmaceutical Science 6(4): 124-127.

Kharia, A.A. & Singhai, A.K. 2013. Screening of most effective variables for development of gastroretentive mucoadhesive nanoparticles by Taguchi design. ISRN Nanomaterials 2013: 1-8.

Kumar, S.P., Latheef, K.A. & Remashree, A.B. 2014. Ethnobotanical survey of diuretic and antilithiatic medicinal plants used by the traditional practitioners of Palakkad District. International Journal of Herbal Medicine 2(2): 52-56.

Liu, Y., Chen, Y., Liao, B., Luo, D., Wang, K., Li, H. & Zeng, G. 2018. Epidemiology of urolithiasis in Asia. Asian Journal of Urology 5(4): 205-214.

Madike, L.N., Takaidza, S. & Pillay, M. 2017. Preliminary phytochemical screening of crude extracts from the leaves, stems, and roots of Tulbaghia violacea. International Journal of Pharmacognosy and Phytochemical Research 9(10): 1300-1308.

Mikawlrawng, K., Kumar, S. & Vandana. 2014. Current scenario of urolithiasis and the use of medicinal plants as antiurolithiatic agents in Manipur (North East India): A review. International Journal of Herbal Medicine 2(1): 1-12.

Mumtaz, F., Raza, S.M., Ahmad, Z., Iftikhar, A. & Hussain, M. 2014. Qualitative phytochemical analysis of some selected medicinal plants occurring in local area of Faisalabad, Pakistan. Journal of Pharmacy and Alternative Medicine 3(3): 5-10.

Nouri, A.I. & Hassali, M.A. 2018. Assessment of the prevalence of kidney stone disease in a Malaysian teaching hospital. African Journal of Urology 24(3): 180-185.

Ong, H.C. & Norzalina, J. 1999. Malay herbal medicine in Gemencheh, Negeri Sembilan, Malaysia. Fitoterapia 70(1): 10-14.

Pandey, A. & Tripathi, S. 2014. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry 2(5): 115-119.

Phatak, R.S. & Hendre, A.S. 2015. In-vitro antiurolithiatic activity of Kalanchoe pinnata extract. International Journal of Pharmacognosy and Phytochemical Research 7(2): 275-279.

Rahim, N.F.A., Muhammad, N., Abdullah, N. & Talip, B.A. 2018. Synergistic effect of polyherbal formulations on DPPH radical scavenging activity. Journal of Science and Technology 10(2): 116-121.

Ramos, G.Q., Da Fonseca De Albuquerque, M.D., Ferreira, J.L.P., Cotta, E.A. & Da Fonseca Filho, H.D. 2016. Wettability and morphology of the leaf surface in cashew tree from the Amazon, Northern Brazil. Acta Scientiarum - Biological Sciences 38(2): 215-220.

Sharifa, A.A., Jamaludin, J., Kiong, L.S., Chia, L.A. & Osman, K. 2012. Anti-urolithiatic terpenoid compound from Plantago major Linn. (ekor anjing). Sains Malaysiana 41(1):  33-39.

Shi, J., Yu, J., Pohorly, J., Young, J.C., Bryan, M. & Wu, Y. 2003. Optimization of the extraction of polyphenols from grape seed meal by aqueous ethanol solution. Journal of Food Agriculture & Environment 1(2): 42-47.

Shukla, A.K., Shukla, S., Garg, A. & Garg, S. 2017. A review on anti-urolithiatic activity of herbal folk plants. Asian Journal of Biomaterial Research 3(2): 1-11.

Sreenevasan, G. 1990. Urinary stones in Malaysia - its incidence and management. The Medical Journal of Malaysia 45(2): 92-112.

Tiwari, A., Soni, V., Londhe, V., Bhandarkar, A., Bandawane, D. & Nipate, S. 2012. An overview on potent indigenous herbs for urinary tract infirmity: Urolithiasis. Asian Journal of Pharmaceutical and Clinical Research 5(1): 7-12.

Uma, D.B., Ho, C.W. & Wan Aida, W.M. 2010. Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) leaves. Sains Malaysiana 39(1): 119-128.

 

*Corresponding author; email: norhayatim@uthm.edu.my

 

 

 

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