Sains Malaysiana 34(1): 17-20 (2005)

 

Potential of Malaysian Local Honey as an Antibacterial Agent

(Potensi Madu Tempatan Malaysia sebagai Agen Antibakteria)

 

 

Ainul Hafiza A.H. & Yusof, N.

Malaysian Institute for Nuclear Technology Research (MINT)

43000 Kajang, Selangor D.E.,Malaysia

 

Maimon, A.

School of Environment & Natural Resource Sciences

Faculty of Science and Technology

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E., Malaysia

 

 

 

ABSTRACT

 

A laboratory study was carried out to study the microbiological quality of local honey and its potential as an antibacterial agent. Five local honeys namely, Belimbing, Gelam (Sample 1,2, and 3), Durian (Sample 1 and 2), Kelapa and Tualang were studied. Microbial colony count of each sample was determined using filtration method. Screening for antibacterial action against Staphylococcus aureus was carried out using agar diffusion method. The result showed that all samples had low colony counts ranging from 37 cfu/ml in Kelapa honey to 161 cfu/ml in Durian honey (Sample 2). All samples showed a very clear zone of inhibition for bacterial growth and there was significant difference (ANOVA, p<0.05) among the samples. Belimbing honey showed the biggest diameter (2.1cm) and Gelam honey – Sample 3 had the smallest (1.7 cm).

 

Key words: honey, antibacterial, colony count

 

 

ABSTRAK

 

Kajian ini dijalankan untuk mengkaji kualiti mikrobiologi madu tempatan dan keupayaannya sebagai agen antibakteria. Lima madu tempatan iaitu Belimbing, Gelam (Sampel 1, 2, dan 3), Durian (Sampel 1 dan 2), Kelapa dan Tualang telah dikaji. Bilangan koloni mikrob ditentukan menggunakan kaedah turasan. Sifat antibakteria terhadap Staphylococcus aureus pula dikaji menggunakan kaedah serapan agar. Keputusan menunjukkan bahawa semua sampel mempunyai bilangan koloni yang rendah sekitar 37 cfu/ml dalam madu Kelapa hingga 161 cfu/ml dalam madu Durian – Sampel 2. Semua sampel menunjukkan kesan rencatan pertumbuhan bakteria dan terdapat perbezaan ketara (ANOVA, p<O.05) di antara sampel. Madu Belimbing menunjukkan ukuran diameter terbesar (2.1cm) dan madu Gelam -Sampel 3 yang terkecil (1.7 cm).

 

Kata kunci: madu, antibakteria, bilangan koloni

 

 

REFERRENCE/RUJUKAN

 

Al-Jabri A.A., Nzeako B., Mahrooqi Z., Al-Naqdy A. & Nsanze H., 2003. In-vitro Antibacterial activity of Omani and African Honey. British Journal of Biomedical Sciences 60 (1): 1-4.

Cooper, R.A., Molan, P.C., & Harding, K.G. 1999. Antibacterial Activity of honey against strains of Staphyloccocus aureus from infected wounds. Journal of The Royal Society of Medicine 92(6): 283-285.

De Centorbi O.P., Satores S.E., Alcaraz C.E., Centorbi H.J. & Fernandez R. 1997. Detection of Clostridium botulinum spores in honey. Review Argent of Microbiology 29(3): 147­151.

Efem, E.E.S., 1988. Clinical observation on the wound healing properties of honey. British Journal Of Surgery 75: 679-681.

Karnaruddin M. Y. 1997. The antibacterial factors in Malaysia Apis Cerana honey. Proceeding of the International Conference on tropical bees & the environment. Beenet Asia: Universiti Pertanian Malaysia.

Migdal W., Owczarczyk H.B., Kedzia B., Holderna-Kedzia E., & Madajczyk D. 2000. Microbiological decontamination of natural honey by irradiation. Radiation Physics and Chemistry 57: 285-288.

Molan P.C. 1999a. Why honey is effective as an medicine. 1. its use in modern medicine. Bee World 80(2): 80-82.

Molan P.C. 1999b. The role of honey in the management of wound. Journal of Wound Care 8: 423-426.

Molan P.C. & Allen K.L. 1996. The effect of gamma-irradiation on the antibacterial activity of honey. Journal of Pharmacy And Pharmacology 48(11): 1206-1209.

Schocken-Iturrino R.P., Carneiro M.C., Keto E., Sarbara J.O., & Gerbasi L., E., 1999. Study of the presence of the spores of Clostridium botulinum in honey in Brazil. FEMS Immunology Medical Microbiology 24(3): 379-382 .

Snowdon J.A., & Cliver D.O. 1996.  Microorganisms in honey. International Journal of Food Microbiology, 31(1-3): 1-26.

Subrahmanyam M., Archan H., & Pawar S.G., 2001. Antibacterial activity of honey on bacteria isolated from wounds. Annals of Burns & Fire Disaster 14(1): 1-4.

Wilix D.J., Molan P.C. & Harfoot C.G. 1992.  A Comparison of the sensitivity of Wound-Infecting species of nacteria to the antibacterial activity of manuka honey & other honey. Journal of Applied Bacteriology 73(5): 388-394.

Yusof  N., Haryati J., Salahbiah A.M. Foziah A., Mohd. Suhaimi H. Shamsiah A.R., & Zainal R. 2000.  Selection of local honey for wound dressing. Seminar R&D Institut Penyelidikan Teknologi Nuklear Malaysia, 17-19 August.

 

 

 

sebelumnya