Sains Malaysiana 36(2): 233-242 (2007)

Stand Structure and the Genetic Diversity of Koompassia malaccensis and

Dryobalanops aromatica in Unlogged and Logged-over Stands

(Struktur dirian dan kepelbagaian genetik Koompassia malaccensis dan Dryobalanops

aromatica pada dirian yang belum dan yang telah dibalak)

Kim Su Lee, Ratnam Wickneswari & Chee Yen Choong

School of Environmental and Natural Resource Sciences

Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor, Malaysia

Received :  18 November 2004 / Accepted:  9 February 2007

ABSTRACT 

The disturbance level of two nearby logged stands, Compartment 118 and Compartment 69 were studied in Ulu Sedili Forest Reserve, Johor. The mean basal area for trees (trees ³ 1 cm dbh) in logged stand of Compartment 118 showed 51% reduction in comparison to immediately before logging of the same stand. A similar level of reduction (47%) was observed for mean density of trees in Compartment 118. However, the mean basal area and mean density of tree were higher in 50-year logged Compartment 69 (21% and 122% respectively) compared to Compartment 118 before logging. Concurrently, we examined the effects of logging on genetic diversity of seedling, sapling and mature trees of two important timber species, Koompassia malaccensis and Dryobalanops aromatica using M13 universal primer (multilocus minisatellite DNA) and three other universally-primed primers. Mature trees of K. malaccensis showed 39% reduction in Shannon diversity index (H) in Compartment 69 compared to Compartment 118 before logging detected by M13 universal primer. This may be attributed to the small sample size of the species in Compartment 69. Reduction in H and polymorphic loci (P) for K. malaccensis was higher in seedlings, 5% and 56% respectively in Compartment 69 compared to mature trees (3% and 23% respectively). Contrastingly for seedlings and saplings of D. aromatica, increment in H was higher in Compartment 69 than Compartment 118 immediately after logging, which was 25% and 14% for seedlings while 21% and 14% for saplings, detected by M13 universal primer. The increased genetic diversity detected for this species may be due to its high density in Compartment 118 and Compartment 69. Loss in alleles caused a decline in H of K. malaccensis, especially mature trees in Compartment 69. On the other hand, H of D. aromatica was not affected by loss of alleles.

Keywords: Selective logging; relative disturbance; genetic diversity; Koompassia malaccensis; Dryobalanops  aromatica

ABSTRAK 

Tahap gangguan ke atas dua dirian pembalakan bersebelahan, Kompartmen 118 dan Kompartmen 69, di dalam Hutan Simpan Ulu Sedili, Johor telah dikaji. Min keluasan basal pokok (pokok ³ 1 cm dbh) di dirian terbalak Kompartmen 118 menunjukkan penurunan 51% berbanding min keluasan basal pokok sejurus sebelum dibalak di dirian tersebut. Min densiti pokok juga menunjukkan tahap penurunan yang sama (47%) di Kompartmen 118. Namun, min basal pokok dan min densiti pokok adalah lebih tinggi (21% dan 122% masing-masing) di Kompartmen 69 yang dibalak 50 tahun dahulu berbanding Kompartmen 118 sejurus sebelum dibalak. Pada masa yang sama, kami telah mengkaji kesan pembalakan ke atas kepelbagaian genetik anak benih, anak pokok dan pokok matang dua spesies pokok balak yang penting, Koompassia malaccensis dan Dryobalanops aromatica, dengan menggunakan pencetus universal M13 (minisatelit DNA berbilang lokus) dan tiga lagi pencetus universal yang lain. Dalam analasis pencetus universal M13, indeks kepelbagaian Shannon (H) bagi pokok matang K. malaccensis menunjukkan penurunan sebanyak 39% di Kompartmen 69 berbanding Kompartmen 118 sejurus sebelum dibalak. Penurunan ini mungkin disebabkan oleh saiz sampel K. malaccensis yang kecil di Kompartmen 69. Kadar penurunan nilai H dan lokus polimorfik (P) adalah lebih tinggi dalam anak benih (5% dan 56% masing-masing) berbanding pokok matang (3% dan 23% masing-masing) K. malaccensis di Kompartmen 69. Sebaliknya, peningkatan nilai H anak benih dan anak pokok adalah lebih tinggi dalam Kompartmen 69 berbanding Kompartmen 118 sejurus selepas pembalakan bagi pokok balak D. aromatica, iaitu 25% dan 14% bagi anak benih dan 21% dan 14% bagi anak pokok masing-masing untuk Kompartmen 69 dan Kompartmen 118 dirian terbalak. Peningkatan kepelbagian genetik yang dikesan dalam D. aromatica adalah disebabkan oleh kelimpahan pokok balak ini dalam kedua-dua kompartmen tersebut. Kehilangan alel menyebabkan penurunan dalam nilai H pokok K. malaccensis, terutamanya bagi pokok matang dalam Kompartmen 69. Sebaliknya, nilai H bagi pokok D. aromatica tidak dipengaruhi oleh kehilangan alel.

Kata kunci:  Pembalakan terpilih; gangguan relatif; kepelbagaian genetik; Koompassia malaccensis; Dryobalanops aromatica 

REFERENCES 

Appanah, S. 1981. Pollination in Malaysian primary forests. Malaysian Forester 44: 37-42.

Appanah, S., Weinland, G. 1993. Planting quality timber trees in Peninsular Malaysia - a review. Malayan Forest Record No. 38.

Ashton, P.S., Girnish, T.J. & Appanah, S. 1988. Staggered flowering in the Dipterocarpaceae: new insight into floral induction and the evolution of mass fruiting in the aseasonal tropics. American Naturalist 132: 44-66.

Bawa, K.S. & Seidler, R. 1998. Natural forest management and conservation of biodiversity in tropical forests. Conservation Biology 12: 46-55.

Bebeli, P.J., Zhou, Z., Somers, D.J. & Gustafson, J.P. 1997. PCR primed with minisatellite core sequences yields DNA fingerprinting probes in wheat. Theoretical and Applied Genetics 95: 276-283.

Bertault, J-G., Dupuy, B. & Maître, H-F. 1993. Silvicultural research of sustainable management of rainforest. Proceedings of the Tropical Silviculture Workshop, IUFRO Centennial Conference, pp. 1-14. Berlin, 1-3 September 1992.

Bertault, J-G. & Sist, P. 1997. An experimental comparison of different harvesting intensities with reduced-impact and conventional logging in East Kalimantan, Indonesia. Forest Ecology and Management 94: 209-218.

Borhan, M., Johari, B. & Quah, E.S. 1987. Studies on logging damage due to different methods and intensities of harvesting in a hill dipterocarp forest of Peninsular Malaysia. Malaysian Forester 50 (1-2): 135-147.

Bulat, S.A., Lübeck, M., Mironenko, N., Jensen, D.F. & Lübeck, P.S. 1998. UP-PCR analysis and ITS, ribotyping of strains of Trichoderma and Gliocladium. Mycology Research 8: 933-943.

Bulat, S.A., Mironenko, M.V., Lapteva, M.N. & Strelchenko, P.P. 1994. Polymerase chain reaction with universal primers (UP PCR) and its applications to plant genome analysis. In: Adams, R.P., Miller, J.S., Golenberg, E.M. & Adams, J.E. (Eds.). Conservation of plant gene II: Utilization of ancient and modern DNA. Missouri Botanical Garden, pp. 113-129.

Cannon, C.H., Peart, D.R. & Leighton, M. 1998. Tree species diversity in commercially logged Bornean rainforest. Science 281: 1366-1368.

Chong, D.K.X., Yeh, F.C., Aravanopoulos, F.A. & Zsuffa, L. 1995. DNA fingerprinting willows (Salix L.) using polymerase chain reaction with the M13 universal primer. Scandinavian Journal of Forest Research 10: 27-31.

Curtis, J.T. & McIntosh, R. 1951. Upland forest communities in the prairie forest border region of Wisconsin. Ecology 32: 476-496.

Davies, S.J. 1998. Tropical ecosystem: environmental impacts. In:Ghazally, I. (ed.), Biodiversity Conservation in ASEAN: Emerging Issues & Regional Needs. ASEAN Academic Press Ltd, England, pp. 14-41.

Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.

Epplen, J.T. 1988. On Simple repeated GATA/GACA sequences I animal genomes: a critical reappraisal. Journal of Heredity 79: 409-417.

Farris, M.A. & Mitton, J.B. 1984. Population density, outcrossing rate and heterozygote superiority in Ponderosa pine. Evolution 38: 1151-1154.

Given, D.R. 1994. Principles and practice of plant conservation. Portland, Oregan, Timber Press.

Guariguata, M.R. 1998. Response of forest tree saplings to experimental mechanical damage in lowland Panama. Forest Ecology and Management 102: 103-111.

ITTO. 1992. ITTO guidelines for the sustainable management of natural tropical forest. ITTO Policy Development Series No. 1.

Jeffreys, A.J., Wilson, V. & Thein, S.L. 1985. Hypervariable 'minisatellite' regions in human DNA. Nature 314: 67-73.

Johns, A.D. 1988. Effects of ‘selective’ timber extraction on rain forest structure and composition and some consequences for frugivores and folivores. Biotropica 20: 31-37.

King, L.M. & Schaal, B.A. 1989. Ribosomal DNA variation and distribution in Rudbeckia missouriensis. Evolution 43: 1117-1119.

Kitamura, K., Mohamad Yusof, A.R., Ochiai, O. & Yoshimaru, H. 1994. Estimation of outcrossing rate on Dryobalanops aromatica Gaertn. f. in primary and secondary forests in Brunei, Borneo, Southeast Asia. Plant Species Biology 9: 37-41.

Konuma, A., Y. Tsumura, C.T. Lee, S.L. Lee & T. Okuda. 1999. Estimation of gene flow in the tropical-rainforest tree Neobalanocarpus heimii (Dipterocarpaceae), inferred from paternity analysis. Molecular Ecology 9: 1843-1852.

Ledig, F.T. 1988. The conservation of diversity in forest trees. Bioscience 38: 471-479.

Lee, C.T., Wickneswari, R., Mahani, M.C. & Zakri, A.H. 2002a. Effects of selective logging on the genetic diversity of Scaphium macropodum. Biological Conservation 104 (1): 101-118.

Lee, C.T., Wickneswari, R., Mahani, M.C. & Zakri, A.H. 2002b. Maintenance of genetic diversity in Parkia speciosa in logged-over forests. Journal of Tropical Forest Science 14(2): 163-178.

Lee, S.L. 2001. Mating system parameters of Dryobalanops aromatica Gaertn. f. (Dipterocarpaceae) in three different forest types and a seed orchard. Heredity 85: 338-345.

Liengsiri, C., Boyle, T.J.B. & Yeh, F.C. 1998. Mating system in Pterocarpus macrocarpus Kurz. In Thailand. Journal of Heredity 89: 216-221.

Lim, L.S., R. Wickneswari, S.L. Lee & A. Latiff. 2002. Genetic variation of Dryobalanops aromatica Gaertn. F. (Dipterocarpaceae) in Peninsular Malaysia using microsatellite DNA markers. Forest Genetics 92(2): 127-138.

Loveless, M.D. 1992. Isozyme variation in tropical trees: patterns of genetics organisation. In: Adam, W.T., Strauss, S.H., Copes, D.L., Griffin, A.R. (eds.). Population genetics of forest trees. Proceedings of the international symposium on population genetics of forest trees,pp. 67-94. Corvalis, Oregan, USA, July 31 – August 2, 1990.

Lübeck, M., Alekhina, I.A., Lübeck, P.S., Jensen, D.F. & Bulat, S.A. 1999. Delineation of Trichoderma harzianum into two different genotypic groups by highly robust fingerprinting method, UP-PCR and UP-PCR product cross-hybridization. Mycology Research 103(3): 289-298.

Murawski, D.A., Gunatilleke, I.A.U.N. & Bawa, K.S. 1994.The effect of selective logging on inbreeding in Shorea megistophylla (Dipterocarpaceae) from Sri Lanka. Conservation Biology 8: 997-1002.

Narayanan, I.V. & Swarupanandan, K. 1996. Regeneration dynamics and sylvigenesis in the moist deciduous forests of southwest India. New Forests 11: 186-205.

Nason, J.D., Aldrich, R. & Hamrick, J.L. 1997. Dispersal and the dynamics of genetic structure in fragmented tropical tree populations. In: Laurance, W. F., Bierregaard, R. O., Jr (eds.). Tropical forest remnants: Ecology, management, and conservation of fragmented communities. University of Chicago Press, Chicago, IL, pp. 304-320.

Nicholson, D.I. 1958. A analysis of logging damage in tropical rainforest, North Borneo. Malaysian Forester 21(4): 235-245.

Prober, S.M. & Brown, A.H.D. 1994. Conservation of the grassy white box woodlands: population genetics and fragmentation of Eucalyptus albens. Conservation Biology 8: 1003-1013.

Raijmann, l.E.L., Van Ginkel, W.E., Heckel, D.G. & Menken, S.B.J. 1994. Genetic variation and outcrossing rate in relation to population size in Gentiana pneumonanthe L. Heredity 8: 1014-1026.

Soerianegara & Lemmens, R.H.M.J. 1994. Timber trees: major commercial timbers. Plant Resources of South-East Asia No. 5(1), Bogor, Indonesia.

Symington, C.F. 1943. Foresters' Manual of Dipterocarps. Malayan Forest Records No. 16. Malaysia, Universiti Malaya.

Tautz, D. 1989. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Research 17: 6463-6471.

Templeton, A.R. 1995. Biodiversity at the molecular genetic level: experiences from disparate macroorganisms. In: Hawksworth, D.L. (ed.). Biodiversity, measurement and estimation. London, Chapman & Hall, pp. 59-64.

Thang, H.C. 1988. Selective management system: Concept and practice (Peninsular Malaysia). Forest Department Headquaters, Kuala Lumpur.

van Treuren, R., bijlsma, R., van Delden, W. & Ouborg, N.J. 1991. The significance of genetic erosion in the process of extinction. I. Genetic differentiation in Salvia pretensis and Scabiosa columbaria in relation to population size. Heredity 66: 181-189.

Vasek, F.C. & Harding, J. 1976. Outcrossing in natural population. V. analysis of outcrossing, inbreeding and selection in Clarkia exilis and Clarkia temboriensis. Evolution 30: 403-411.

Wan Razali, W. 1989. Summary of growth and yield studies in tropical mixed forests. Proj. Pap. UNDP/RAS/86/049, Forest Research Institute, Malaysia, Kepong, FRIM Report No. 49, pp. 17-83.

Whitmore, T.C. 1983. Secondary succession from seed in tropical rain forests. Commonwealth Forestry Bureau, Forestry Abstracts 44(12): 767-779.

Wickneswari, R. & Boyle, T.J. 2000. Effects of logging and other forms of harvesting on genetic diversity in humid tropical forests. In: Young, A., Boshier, D., Boyle, T. (eds.). Forest conservation genetics: principles and practice.Australia, CSIRO Publishing, pp. 115-122.

Wickneswari, R., Lee, C.T., Norwati, M. & Boyle, T.J.B. 1997a. Effects of logging on the genetic diversity of six tropical rainforest species in a regenerated mixed dipterocarp lowland forest in Peninsular Malaysia. Paper presented at CIFOR Wrap-Up Workshop on Impact of Disturbance, Bangalore, India, August 1997.

Wickneswari, R., Lee, C.T., Norwati, M. & Boyle, T.J.B. 1997b. Immediate effects of logging on genetic diversity of five tropical rainforest species in a ridge forest in Peninsular Malaysia. Paper presented at CIFOR Wrap-Up Workshop on Impact of Disturbance, Bangalore, India, August 1997.

Wyatt-Smith, J. 1963. Manual of Malayan Silviculture for Inland Forests. Malayan Forest Records No. 23(1).

Wyatt-Smith, J. & Foenander, E.C. 1962. Damage to the regeneration as the result of logging. Malaysian Forester 25(1): 40-44.

Young, A., Boyle, T. & Brown, T. 1996. The population genetic consequences of habitat fragmentation for plants. TREE 11: 413-418.

Zhou, Z., Bebeli, P.J. Somers, D.J. & Gustafson, J.P. 1997. Direct amplification of minisatellite-region DNA with VNTR core sequences in genus Oryza. Theoretical and Applied Genetics 95: 942-949.