Sains Malaysiana 41(3)(2012): 333–337

 

Chemical Components of Marine Sponge Derived Fungus Fusarium proliferatum

Collected from Pulau Tinggi, Malaysia

(Komponen Kimia Kulat Fusarium proliferatum diperolehi daripada Span Marin

di Perairan Pulau Tinggi, Malaysia)

 

 

Z. Mosadeghzad & G. H. N. Farahani

Department of Chemistry, Faculty of Science, Islamic Azad University-Sharekord Branch

166 Shahrekord-Iran

 

Zuriati Zakaria

School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E. Malaysia

 

Asmat, A., Gires, U. & Wickneswari. R.

School of Bio Sciences and Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E. Malaysia

 

Pittayakhajonwut, P.

National Centre for Genetic Engineering and Biotechnology (BIOTEC)

National Science and Technology Development Agency (NSTDA)

113, Paholyothin Road, Klong 1, Klong Luang, Pathum Thani 12120 Thailand

 

Diserahkan: 26 April 2011 / Diterima: 13 September 2011

 

ABSTRACT

 

Marine fungus Fusarium proliferatum derived from marine sponge collected along Pulau Tinggi, Malaysia was cultivated on Potato Dextrose Broth and incubated for 7 days at 30oC. The liquid cultures were then extracted using ethyl acetate. The crude extract was investigated for its anti-microbial activity and was passed through Sephadex column and the fractions were collected. Reverse phase HPLC was used to monitor the component of crude extract. HPLC guided purification of crude extract resulted in the isolation of linoleic acid, 4-hydroxy phenethyl alcohol, 2,5-furandimethanol and adenosine. Their structures were elucidated by spectroscopic methods.

 

Keywords: Antimicrobial activity; Fusarium proliferatum; marine fungi

 

ABSTRAK

 

Kulat marin Fusarium proliferatum yang diperolehi daripada span marin dikutip di perairan Pulau Tinggi, Malaysia telah dieramkan pada Agar Potato Dextrose (PDA) pada 30°C selama 7 hari. Kultur cecair diekstrak dengan pelarut etil asetat. Ekstrak mentah dikaji untuk aktiviti antimikrob dan dimasukkan ke dalam turus Sephadex dan fraksi dikumpulkan. HPLC fasa terbalik digunakan untuk memantau komponen ekstrak mentah. Penulenan ekstrak mentah berpandukan HPLC telah menghasilkan pemencilan asid linoleik, alcohol 4-hidroksifenetil, 2,5-furan dimetanol dan adenosine. Struktur telah ditentukan dengan kaedah spektroskopi.

 

Kata kunci: Aktiviti antimikrob; Fusarium proliferatum; kulat marin

 

 

RUJUKAN

 

Chen, H., Fujita, M., Feng, Q., Clardy, J. & Fink, G.R. 2004. Proc. Natl. Acad. Sci USA 101: 5048-5052.

Colwell, R.R. 2002. Fufilling the promise of biotechnology. Biotechnol. Adv. 20: 215-228.

Dominguez, T.J. 2004. It’s not a spider bite, its community acquired methicililn-resistant Staphylococcus aureus. J. Am. Board. Fam. Pract. 17: 220-226.

Faulkner, D.J. 2000a. Highlights of marine natural products chemistry (1972-1999). Nat. Prod. Rep. 17: 1-6.

Faulkner, D.J. 2000b. Marine pharmacology. Antonie van Leeuwenhoek 77: 135-145.

Fenical, W. & Jensen, P.R. 1993. Marine microorganisms: a new biomedical resource. In Marine Biotechnology, Vol 1, edited by Attaway, D.H., Zaborsky, O.R. New York: Plenum Press.

Gallo, M.L., Seldes, A.M. & Cabrera, G.M. 2004. Antibiotic long-chain and α, β-Unsaturated aldehydes from the culture of the marine fungus Cladosporium sp. Biochem. Syst. Ecol. 32: 545-551.

Laatsch, H. 2003. A Data Base for Rapid Structural Determination of Microbial Natural Products, 2003 and annual updates, Chemical Concepts, Weinheim, Germany; http://www.user. gwdg. de/~ucoc/laatsch/AntiBase. htm.Malakoff, D. 1997. Extinction on the high seas. Science 277:486-488.

Nagaraju, U., Bhat, G., Kuruvila, M., Pai, G.S., Jayalakshmi & Babu R.P. 2004. Methicillin-resistant Staphylococcus aureus in community-acquared pyoderma. Int. J. Dermatol. 43: 412-414.

Neu, H.C. 1992. The crisis in antibiotic resistance. Science 257: 1064-1073.

Pomponi, S.A. 1999. The bioprocesstechnological potential of the sea. J. Biotech. 70: 5-13.

Pouchert, C.J. & Behnke, J. 1993. The Aldrich Library of 13C and 1H FT NMR Spectra. 1st ed. Vol. 1. P785/B. Rovirosa, J., Marrero, D.A., Darias, J., Painemal, K. & Martin,

S.A. 2006. Secondary metabolites from marine Penicilium brevicompactum. Journal of Chilean Chemical Society 51: 1-6.

Serdyuk, O.P., Smolygina, L.D, Muzafarov, E.N., Adanin, V.M. & Arinbasarov, M.U. 1995. FEBS Lett. 10: 365.

 

*Pengarang untuk surat-menyurat; email: zmzad79@gmail.com

 

 

 

 

sebelumnya