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Sains Malaysiana 42(1)(2013):
33–38
Plasma Fibronectin in Saliva of Women with History of
Miscarriage(s) and Periodontal Disease - a Pilot Study
(Fibronektin Plasma di dalam Air Liur Wanita Mengandung dengan Sejarah
Keguguran dan Penyakit Periodontitis Kronik- Kajian Permulaan)
Farha
Ariffin*
Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
Salmah
Kamin
KPJ Selangor Specialist Hospital, 40300 Shah Alam, Selangor, Malaysia
Jamiyah
Hassan
Department of obstetrics and gynaecology, University of Malaya Medical Centre 50900
Kuala Lumpur, Malaysia
Haliza
Hasan
Faculty of computer Science and Mathematics, Universiti Teknologi MARA
40450
Shah Alam, Selangor, Malaysia
Diserahkan:
19 Disember 2011/Diterima: 18 Mei 2012
ABSTRACT
The objective of this study was to establish the association of
plasma fibronectin in saliva of pregnant women with history of miscarriage(s). A
total of 25 pregnant women with history of miscarriage(s) were recruited at the
Antenatal Clinic of University Malaya Medical Centre (UMMC), Kuala Lumpur as
the test group and 16 pregnant women without any history of miscarriage served
as control. All the subjects fulfilled a set of inclusion and exclusion
criteria. Periodontal examination was performed to determine subjects’
periodontal status. Saliva samples were collected and enzyme-linked
immunosorbent assay (ELISA) was used to detect the plasma
fibronectin in these samples. Mann-Whitney test was used to determine the
significance of differences seen. The study showed a statistically significant
higher median plasma fibronectin level in the saliva samples of the test group
with a median level of 0.10 μg/mL compared with the control group with a
mean value of 0.00 μg/mL (p-value =0.023). However, there was no significant difference
in the level of plasma fibronectin in the saliva samples of pregnant women with
chronic periodontitis compared with the group with healthy gingiva (p-value=
0.118). As a conclusion, there was a positive association between plasma
fibronectin in saliva of women and history of miscarriages compared with the
control group in this case study.
Keywords: Miscarriage; periodontal disease;
plasma fibronectin; saliva
ABSTRAK
Objektif kajian ini adalah untuk melihat hubung
kait antara fibronektin plasma di dalam air liur ibu mengandung dengan sejarah
keguguran. Seramai 25 ibu mengandung
dengan sejarah keguguran di Klinik Ibu Mengandung Pusat Perubatan Universiti
Malaya, Kuala Lumpur telah dikenal pasti sebagai kumpulan kajian manakala
seramai 16 lagi ibu mengandung tanpa riwayat keguguran dijadikan kumpulan
kawalan. Kesemua subjek kajian menepati kriteria yang telah
ditetapkan. Pemeriksaan periodontium telah dilakukan
bagi mengenal pasti status kesihatan periodontium mereka. Sampel saliva
mereka dianalisis menggunakan ujian ‘enzyme-linked immunosorbent assay’ (ELISA)
untuk mengesan kandungan fibronektin plasma. Ujian
Mann-Whitney digunakan bagi menganalisis data. Keputusan mendapati aras purata fibronektin plasma di dalam kumpulan kajian
adalah melebihi berbanding purata fibronektin plasma dalam kumpulan kawalan. Walau bagaimanapun, tiada perbezaan ketara yang ditemui bagi purata
fibronektin plasma antara kumpulan subjek yang mempunyai penyakit periodontitis
kronik berbanding subjek yang mempunyai periodontium yang sihat. Kesimpulannya, kajian ini menunjukkan bahawa terdapat hubung kait yang positif
antara aras fibronektin plasma di dalam saliva subjek
dengan riwayat keguguran.
Kata kunci: Air liur; fibronektin plasma;
keguguran; penyakit periodontium
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*Pengarang
untuk surat-menyurat; email: drfarha@salam.uitm.edu.my
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