Sains Malaysiana 42(5)(2013): 635–642

 

Effects of GnRHa on Plasma Sex Steroid Hormones of

River Catfish Hemibagrus nemurus(Valenciennes 1840)

(Kesan GnRHa Terhadap Hormon Seks Steroid Plasma Ikan Baung Hemibagrus nemurus(Valenciennes 1840))

 

FatimatAdenike Adebiyi, S itiS haporSiraj*, S harrAzniHarmin & Annie Christianus

Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia

43400 UPM Serdang, Selangor, Malaysia

 

Diserahkan: 12 Mac 2012/Diterima: 4 Oktober 2012

 

ABSTRACT

The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 μg/kg, 20 μg/kg and 50 μg/kg body weight (BW) of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 μg/kg. Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 μg/kg and 50 μg/kg BW GnRHa treatments and a dose of 5 μg/kg BW GnRHa produced a slow response to steroidogenesis. Treatment with 50 μg/kg BW GnRHa produced the best result. The highest level of testosterone, 11-ketotestosterone and 17β-estradiol were observed at 24 h for all treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus. Therefore, it can be used as an inducing agent for the control of reproduction in H. nemurus.

 

Keywords: Gonadotropin releasing hormone analogue (GnRHa); Hemibagrus nemurus; testosterone; 11-ketotestosterone; 17β-estradiol

ABSTRAK

Kesan analog hormon lepasan gonadotropin (GnRHa) terhadap hormon seks steroid plasma ikan baungHemibagrus nemurus telah dikaji. Enzim berkaitan imunosorben asei (ELISA) telah digunakan untuk mengukur hormon. Ikan telah dirawat dengan salina (kawalan) dan GnRHa pada pelbagai dos 5 μg/kg, 20 μg/kg dan 50 μg/kg berat badan (BW) ikan. Sampel darah telah dikumpul pada 0, 6, 12 dan 24 jam selepas suntikan hormon. Keputusan menunjukkan bahawa GnRHa meningkatkan hormon seks steroid plasma walaupun pada dos yang rendah, 5 μg/kg. Peningkatan yang signifikan (p< 0.05) dalam tahap seks steroid plasma telah diperhatikan pada rawatan 20 μg/kg dan 50 μg/kg BW GnRHa sementara dos 5 μg/kg BW GnRHa menghasilkan tindak balas yang perlahan untuk steroidogenesis. Rawatan GnRHa pada dos 50 μg/kg BW menunjukkan hasil yang terbaik. Tahap tertinggi testosteron, 11-ketotestosterone dan-estradiol 17β telah diperhatikan pada 24 jam untuk semua rawatan. Keputusan menunjukkan bahawa GnRHa meningkatkan pengeluaran steroid dalam plasma H. nemurus. Oleh itu, ia boleh digunakan sebagai ejen pendorong untuk kawalan pembiakanH. nemurus.

 

Kata kunci: Analog hormon lepasan gonadotropin (GnRHa); Hemibagrus nemurus; testosteron; 11-ketotestosterone; 17β-estradiol

RUJUKAN

Amano, M., Urano, A. & Aida, K. 1997. Distribution and function of gonadotropin releasing hormone in the teleost brain. Zoological Science 14: 1-11.

Ando, H. & Urano, A. 2005. Molecular regulation of gonadotropin secretion by gonadotropin releasing hormone in salmonid fishes. Zoological Science 22: 379-389.

Cuisset, B., Pradelles, P., Kime, D.E., Kuhn, E.R., Babin, P. & Le Menn, F. 1994. Enzyme immuno assay for 11-ketotestosterone using acetylcholinestarase as label. Application to measurement of 11-ketotestosterone in plasma of Siberian sturgeon. Comparative Biochemistry and Physiology C 108: 229-241.

Espinoza, C., Perea, A., Buitron, B., Cisneros, P., Catcoparco, C., Alberro, A. & Vizzian, O.D. 2010. Induction of spawning and spermiation in captive Peruvian anchovy Engraulis ringens(Jennyns) using GnRH analogue injection. Latin American Journal of Aquatic Research 38: 234-241.

Greenwood, L.N., Scott, A.P., Vermeirssen, E.L.M., Mylonas, C.C. & Pavlidis, M. 2001. Plasma steroids in mature common dentex (Dentex dentex) stimulated with gonadotropin releasing hormone agonist. General and Comparative Endocrinology 123: 1-12.

Harmin, S.A. & Crim, L.W. 1993. Influence of gonadotropin releasing hormone analogue on plasma sex steroid profiles and milt production in male winter flounder, Pseudopleuronectes americanus (Walbaum). Fish Physiology and Biochemistry 10: 399-407.

Joy, K.P., Senthikumaran, B. & Sudhakumari, C.C. 1998. Periovulatory changes in hypothalamic and pituitary monoamines following GnRH analogue treatment in the catfish Heteropneustes fossilis: Astudy correlating changes in plasma hormone profiles. Journal of Endocrinology 156: 365-372.

Kime, D.E. 1993. Classical and non-classical reproductive steroids in fish. Reviews in Fish Biology and Fisheries 3: 160-180.

Meng-Umphan, K., Manosroi, J. & Manosroi, A. 2006. Successful artificial breeding of the Mekong giant catfish (Pangasianodon gigas, Chevey) reared in earthened ponds by boostering with gonadotropin releasing hormone analogue. Asian Fisheries Science 19: 149-155.

Mesomya, W., Cuptapun, Y., Jittanoonta, P., Hengsawadi, D., Boonvisut, S., Huttayanon, P. & Sriwatana, W. 2002. Nutritional evaluations of green catfish Mystus nemurus. Kasetsart Journal (Natural Science) 36: 69-74.

Mylonas, C.C. & Zohar, Y. 2001. Use of GnRHa-delivery system for the control of reproduction in fish. Reviews in Fish Biology and Fisheries 10: 463-491.

Nash, J.P., Davail-Cuisset, B., Bhattacharyya, S., Suter, H., Le Menn, F. & Kime, D.E. 2000. An enzyme linked immunosorbent assay (ELISA) for testosterone, 17β-estradiol and 17α, 20β-dihydroxy-4-pregnen-3-one using acetylcholinesterase as tracer: Application to measurement of diel patterns in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry 22: 355-363.

Okuzawa, K. 2002. Puberty in teleosts. Fish Physiology and Biochemistry 26: 31-41.

Prat, F., Zanuy, S. & Carrillo, M. 2001. Effects of gonadotropin releasing hormone analogue and pimozide on plasma levels of sex steroids and ovarian development in sea bass (Dicentrarchus labrax, L.). Aquaculture 198: 325-338.

Rainboth, W.J. 1996. Fishes of the Cambodian Mekong. FAO Species Identification Field Guide for Fishery Purposes. Rome: FAO.

Richter, C.J.J., Eding, E.H., Goos, H.J.Th., De Leeuw, R., Scott, A.P. & Van Oordt, P.G.W.J. 1987. The effect of pimozide/LHRHa and 17α-hydroxyprogesterone on plasma steroid levels and ovulation in the African catfish Clarias gariepinus. Aquaculture 63: 157-168.

Rottman, R.W., Shireman, J.V. & Chapman, F.A. 1991a. Hormonal control of reproduction in fish for induced spawning. Southern Regional Aquaculture Centre (SRAC) Publication 424: 1-4.

Rottman, R.W., Shireman, J.V. & Chapman, F.A. 1991b. Hormonal preparation, dosage calculation and injection techniques for induced spawning of fish. Southern Regional Aquaculture Center Publication 425: 1-4.

Schulz, R.W., Van Der Sanden, M.C.A., Bosma, P.T. & Goos, H.J.Th. 1994. Effects of gonadotropin releasing hormone during the pubertal development of the male African catfish (Clarias gariepinus): Gonadotropin and androgen levels in plasma. Journal of Endocrinology 140: 265-273.

Semenkova, T., Barannikova, I., Kime, D.E., Mc Allister, B.G., Bayunova, L., Dyubin, V. & Kolmakov, N. 2002. Sex steroid profiles in female and male stellate sturgeon (Acipenser

stellatus, Pallas) during final maturation induced by hormonal treatment. Journal of Applied Ichthyology 18: 375-381.

Senthilkumaran, B. & Joy, K.P. 2001. Periovulatory changes in catfish ovarian oestradiol - 17β, oestrogen-2-hydroxylase and catechol-o-methyltransferase during GnRH analogue induced ovulation and in-vitro induction of oocyte maturation by catecholoestrogens. Journal of Endocrinology 168: 239-247.

Taufek, N., Harmin, S.A. & Christianus, A. 2009. Effects of chicken-II gonadotropin releasing hormone (CGnRH-II) on plasma steroid hormone, maturation and ovulation in African catfish Clarias gariepinus(Burchell). African Journal of Biotechnology 8: 6700-6709.

Zhuo, Q., Zhang, Y., Huang, W., Liu, X., Li, Y., Zhu, P., Lu, D. & Lin, H. 2011. Gonadotropin releasing hormone analogue multiple injection potentially accelerated testicular maturation of male yellow catfish (Pelteobagrus fluvidracoRichardson) in captivity. Aquaculture Research 42: 1-14.

Zohar, Y. & Mylonas, C.C. 2001. Endocrine manipulations of spawning in cultured fish: From hormones to genes. Aquaculture 197: 99-136.

Zohar, Y, Munoz-Cueto, J.A., Elizur, A. & Kah, O. 2010. Neuroendocrinology of reproduction in teleost fish. General and Comparative Endocrinology 165: 438-455.

 

 

*Pengarang untuk surat-menyurat; email: shapor@putra.upm.edu.my


 

sebelumnya