Sains Malaysiana 43(12)(2014): 1895–1900

 

Antiproliferative Potential of Extracts from Kappaphycus Seaweeds on HeLa Cancer Cell Lines

(Potensi Antiproliferatif Ekstrak Rumpai Laut Kappaphycus ke atas Garisan Sel Kanser HeLa)

 

TIEK YING LAU1, DELBORA FENNY VITTAL2, CASSANDRA SZE YII CHEW1

& WILSON THAU LYM YONG1*

 

1Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia

 

2School of Science and Technology, Universiti Malaysia Sabah, Jalan UMS

88400 Kota Kinabalu, Sabah, Malaysia

 

Diserahkan: 10 Mac 2013/Diterima: 24 April 2014

 

ABSTRACT

A review of the current literature indicates that natural seaweeds are an excellent source of bioactive compounds with antioxidant, antimicrobial and antitumor properties. In the present study, 90% methanolic, 70% acetonic and aqueous extracts from Kappaphycus alvarezii (strains Crocodile, Giant and Brown) and Kappaphycus striatum were used to inhibit the growth of HeLa cell lines. MTS assay was carried out to determine the proliferation of HeLa cells in the presence of different seaweed extracts. Both 500 μg/mL of aqueous and methanolic extracts from K. striatum demonstrated highest anti-proliferative activity against HeLa cells with cell growth inhibition of 53.5 and 43.7%, respectively. Treatment with the aqueous extracts from three strains of K. alvarezii did not show any growth inhibition against HeLa cell lines. The acetonic extract of Kappaphycus seaweeds exhibited a very poor cell growth inhibition with inhibitory activity observed under the treatment of 300 to 500 μg/mL of K. alvarezii strain Brown only. Further studies are suggested to identify and purify the specific anti-tumoral compounds for potential use in cancer therapy.

 

Keywords: Antiproliferation; growth inhibition; Kappaphycus alvarezii; Kappaphycus striatum

 

ABSTRAK

Suatu kajian kesusasteraan semasa menunjukkan bahawa rumpai laut semula jadi adalah sumber komponen bioaktif yang sangat baik dengan aktiviti antioksidan, antimikrobial dan antitumor. Dalam kajian ini, ekstrak metanol 90%, aseton 70% dan akueus daripadaKappaphycus alvarezii (strain Buaya, Giant dan Brown) danKappaphycus striatum telah digunakan untuk merencat pertumbuhan garisan sel HeLa. Asai MTS telah dijalankan untuk mengkaji pertumbuhan sel HeLa dalam kehadiran pelbagai ekstrak rumpai laut. Kedua-dua 500 μg/mL ekstrak akueus dan metanol daripadaK. striatum menunjukkan aktiviti antiproliferasi yang tertinggi terhadap sel HeLa masing-masing dengan perencatan pertumbuhan sel sebanyak 53.5 dan 43.7%. Rawatan dengan ekstrak akueus daripada tiga strain K. alvarezii tidak menunjukkan perencatan pertumbuhan terhadap garisan sel HeLa. Ekstrak aseton daripada rumpai lautKappaphycus mempamerkan perencatan pertumbuhan sel yang lemah dengan aktiviti perencatan hanya boleh diperhatikan dengan rawatan 300 hingga 500 μg/mL daripadaK. alvarezii strain Brown sahaja. Kajian lanjutan adalah dicadangkan untuk mengenal pasti dan menulenkan komponen spesifik antitumor untuk pembangunan terapi kanser.

 

Kata kunci: Antiproliferasi; Kappaphycus alvarezii; Kappaphycus striatum; perencatan tumbuhan

 

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*Pengarang untuk surat-menyurat; email: wilsonyg@ums.edu.my

 

   

 

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