Sains Malaysiana 44(5)(2015): 651–656

 

Molecular Characterization of Colletotrichum Isolates Associated with Anthracnose of Mango Fruit

(Pencirian Molekul Pencilan Colletotrichum Berkait dengan Antraknos Buah Mangga)

 

LATIFFAH ZAKARIA*, NURUL ZAADAH JUHARI, SUZIANTI ISKANDAR VIJAYA & INTAN SAKINAH MOHD ANUAR

School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia

 

 

Diserahkan: 16 Februari 2014/Diterima: 26 Februari 2015

 

ABSTRACT

Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Chokanan and Harum Manis. Based on the conidial morphology, two morphotypes (I and II) of C. gleosporioides were identified. Based on BLAST search of ITS regions and β-tubulin sequences, majority of the isolates showed 99-100% similarity with Colletotrichum sp. from mango and other hosts and three isolates, 100% similarity with C. asianum. From phylogenetic analysis using maximum likelihood method of combined datasets, the isolates from mango formed three clades, which corresponded to C. gloeosporioides sensu lato and C. asianum. Therefore, the present study showed that the isolates associated with anthracnose of mango belong to C. gleosporioides sensu lato and C. asianum.

 

Keywords: Anthracnose; β-tubulin; Colletotrichum; ITS regions; mango

 

ABSTRAK

Spesies Colletotrichum adalah agen penyebab utama antraknos. Suatu kajian telah dijalankan untuk menentukan identiti spesies Colletotrichum yang berkait dengan antraknos buah mangga (Mangifera indica). Tiga puluh lima pencilan Colletotrichum telah dipencilkan daripada lesi antraknos dua kultivar mangga, Chokanan dan Harum Manis. Berdasarkan morfologi konidium, dua morfotip (I dan II) C. gleosporioides telah dikenal pasti. Berdasarkan carian BLAST kawasan ITS dan gen β-tubulin, majoriti pencilan menunjukkan 99-100% kesamaan dengan Colletotrichum sp. daripada mangga dan perumah yang lain dan tiga pencilan, 100% kesamaan dengan C. asianum. Analisis filogenetik menggunakan kaedah kebolehjadian maksimum gabungan data, menunjukkan kesemua pencilan mangga membentuk tiga klad, yang sejajar dengan C. gloeosporioides sensu lato dan C. asianum. Oleh itu, kajian ini menunjukkan bahawa pencilan yang berkait dengan antraknos mangga adalah C. gloeosporioides sensu lato dan C. asianum.

 

Kata kunci: Antraknos; β-tubulin; Colletotrichum; kawasan ITS; manga

RUJUKAN

 

Arx JA von. 1970. A revision of the fungi classified as Gloeosporium. Bibliotheca Mycologica 24: 1-203.

Bailey, J.A. & Jeger, M.J. 1992. Colletotrichum: Biology, Pathology and Control. Wallingford, United Kingdom: CAB International.

Cai, L., Hyde, K.D., Taylor, P.W.J., Weir, B.S., Waller, J., Abang, M.M., Zhang, J.Z., Yang, Y.L., Phoulivong, S., Liu, Z.Y., Prihastuti, H., Shivas, R.G., McKenzie, E.H.C. & Johnston, P.R. 2009. A polyphasic approach for studying Colletotrichum. Fungal Diversity 39: 183-204.

Damn, U., Baroncelli, R., Cai, L., Kubo, Y., O’Connell, R., Weir, B., Yoshino, K. & Cannon, P.F. 2010. Colletotrichum: Species, ecology and interactions. IMA Fungus 1(2): 161-165.

Freeman, S., Katan, T. & Shabi, E. 1998. Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease 82: 596-605.

Glass, N. & Donaldson, G. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61(4): 1323-1330.

Hyde, K.D., Cai, L., Cannon, P.F., Crouch, J.A., Crous, P.W., Damm, U., Goodwin, P.H., Chen, H., Johnston, P.R., Jones, E.B.G., Liu, Z.Y., McKenzie, E.H.C., Moriwaki, J., Noireung, P., Pennycook, S.R., Pfenning, L.H., Prihastuti, H., Sato, T., Shivas, R.G., Tan, Y.P., Taylor, P.W.J., Weir, B.S., Yang, Y.L. & Zhang, J.Z. 2009. Colletotrichum - names in current use. Fungal Diversity 39: 147-183.

Ilag, L.L., Muid, S., Chye, T.S., Prabawati, S. & Vichitrananda, S. 1994. Postharvest pathology and control diseases. In Papaya- Fruit Development, Postharvest Physiology, Handling and Marketing in ASEAN, edited by M.Y. Rohani, ASEAN Food Handling Bureau, Kuala Lumpur, Malaysia. pp. 83-98.

Johnston, P.R., Dodd, S., Park, D., Massey, B., Charuchinda, B., Waipara, N. & Buckley, T. 2008. Are stable, consistent, reliable, and useful species names possible within Colletotrichum? In Colletotrichum Diseases of Fruit Crops. PreCongress workshop, ICPP 2008, Torino, Italy. pp. 1-9.

Kamle, M., Kumar, P., Gupta, V.K., Tiwari, A.K., Misra, A.K. & Pandey, B.K. 2013. Identification and phylogenetic correlation among Colletotrichum gloeosporioides pathogen of anthracnose for mango. Biocatalysis and Agricultural Biotechnology 2: 285-287.

Krishnapillai, N. & Wilson Wijeratnam, R.S. 2014. First Report of Colletotrichum asianum causing anthracnose on Willard mangoes in Sri Lanka. New Disease Reports 29: 1 doi.org/1 0.5197/j.2044-0588.2014.029.001.

Kwee, L.T. & Chong, K.K. 1994. Diseases and Disorders of Mango in Malaysia. Kuala Lumpur: Tropical Press Sdn. Bhd.

Lima, N.B., Batista, M.V. de A., Morais Jr, M.A. De., Barbosa, M.A.G., Michereff, S.J., Hyde, K.D. & Câmara, M.P.S. 2013. Five Colletotrichum species are responsible for mango anthracnose in northeastern Brazil. Fungal Diversity 61: 75-88.

Mordue, J.E.M. 1971. CMI Description of Plant Pathogenic Fungi and Bacteria. No. 315.

Phoulivong, S., Cai, L., Chen, H., McKenzie, E.H.C., Abdelsalam, K., Chukeatirote, E. & Hyde, K.D. 2013. Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Diversity: 44: 33-43.

Prihastuti, H., Cai, L., Chen, H., McKenzie, E.H.C. & Hyde, K.D. 2009. Characterisation of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity 39: 89-109.

Sharma, G., Kumar, N., Weir, B.S., Hyde, K.D. & Shenoy, B.D. 2013. The ApMat marker can resolve Colletotrichum species: A case study with Mangifera indica. Fungal Diversity 61: 117-138.

Sutton, B.C. 1992. The genus Glomerella and its anamorph Colletotrichum. In Colletotrichum Biology, Pathology and Control, edited by J.A. Bailey & M.J. Jeger. Wallingford, UK: CAB International. pp. 1-26.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. 2011. MEGA5: Molecular evolutioary genetic analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution 28: 2731-2739.

Weir, B.S., Johnston, P.R. & Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115-180.

White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications, edited by Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. San Diego: Academic Press. pp. 315-322.

 

*Pengarang untuk surat-menyurat; email: Lfah@usm.my

 

 

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