Sains Malaysiana 44(5)(2015): 727–733

 

High Expression of Cyclooxygenase-2 in High Grade Human Prostate Adenocarcinoma

(Pengekspresan Tinggi Siklooksigenase-2 dalam Adenokarsinoma Prostat Manusia Gred Tinggi)

 

MOHD ROHAIZAD MD RODUAN1*, NORHAFIZAH MOHTARRUDIN1, CHONG PEI PEI2,

MALINA OSMAN3 & NORAINI MOHD DUSA4

 

1Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia,

43400 Serdang, Selangor Darul Ehsan, Malaysia

 

2Department of Biomedical Sciences, Faculty of Medicine and Health Sciences,

Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

 

3Department of Medical Microbiology & Parasitology, Faculty of Medicine and Health Sciences,

Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

 

4Department of Pathology, Hospital Kuala Lumpur, Jalan Pahang, 50588 Kuala Lumpur

Malaysia

 

Diserahkan: 17 Mei 2013/Diterima: 22 Disember 2014

 

ABSTRACT

Inflammation plays an important role to the process of prostate carcinogenesis by increasing the rate of cell proliferation, which contributes to an aggressive tumour phenotype. Cyclooxygenase-2 (COX-2) has been found overexpressed in various types of cancer cells including prostate. The aim of this study was to investigate the COX-2 expressions in different types of human prostate tissues. Paraffin-embedded prostate tissues from 263 samples were examined for the expression of COX-2 marker by immunohistochemistry method. COX-2 was found highly expressed in prostate adenocarcinoma (p=0.001) as compared to benign and normal tissues. The score of COX-2 expressions in most of normal prostate was weak 49 (77.8%), while only 16 (16%) of BPH showed strong expression. 56 cases (56%) prostate cancer showed strong COX-2 expression. Prostate cancer cases showed significant differences in staining patterns as tumour grade increased. In addition, COX-2 expression was significantly correlated with Gleason score in cancerous tissues. This study suggests that COX-2 overexpression is associated with prostate cancer and higher grade tumour.

 

Keywords: Cyclooxygenase-2 (COX-2); immunohistochemistry; inflammations; overexpression; prostate adenocarcinoma

 

ABSTRAK

Inflamasi memainkan peranan penting dalam proses pembentukan kanser prostat melalui peningkatan kadar pembahagian sel. Inflamasi juga bertanggungjawab dalam pembentukan fenotip kanser prostat yang lebih agresif. Siklooksigenase-2 (COX-2 didapati diekspres lebih tinggi daripada aras biasa dalam pelbagai jenis sel kanser termasuk prostat. Tujuan kajian ini dijalankan adalah untuk mengkaji pengekspresan COX-2 dalam tisu prostat manusia yang berbeza. Sebanyak 263 tisu prostat tertanam parafin telah digunakan untuk mengkaji aras pengekspresan penanda COX-2 melalui kaedah imunohistokimia. Pengekspresan COX-2 didapati lebih tinggi dalam tisu adenokarsinoma prostat (p=0.001) berbanding tisu benigna dan tisu prostat yang normal. Kebanyakan tisu prostat normal mengekspres COX-2 yang lemah 49 (77.8%) dan hanya 16 (16%) tisu BPH menunjukkan pengekspresan COX-2 yang kuat. Manakala, 56 (56%) tisu kanser prostat menunjukkan pengekspresan COX-2 yang kuat. Tisu kanser prostat juga menunjukkan perbezaan corak perwarnaan COX-2 yang signifikan dengan peningkatan gred tumor. Selain itu, pengekspresan COX-2 menunjukkan perhubungan yang signifikan dengan skor Gleason dalam tisu kanser. Kajian ini mencadangkan bahawa terdapat hubungan antara pengekspresan COX-2 yang sangat tinggi dengan kanser prostat dan tumor gred tinggi.

 

Kata kunci: Adenikarsinoma prostat; siklooksigenase-2 (COX-2); imunohistokimia; inflamasi; pengekspresan

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