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Sains Malaysiana 44(7)(2015): 947–955 Pure
Cultivation and Morphological Studies of Four Chaetoceros Taxa
from the Coastal Waters of Pahang, Malaysia (Pengkulturan Tulen
dan Kajian Morfologi Empat Takson Chaetoceros dari Persisiran
Pantai Pahang, Malaysia) M.A.M.
FUAD1*,
N.
MOHAMMAD-NOOR2,
A.K.C.
JALAL2
& B.Y. KAMARUZZAMAN2 1Institut Kesihatan
Umum, Kementerian Kesihatan Malaysia, Jalan Bangsar, 50590 Kuala
Lumpur Malaysia 2Kulliyyah of Science,
International Islamic University Malaysia, Jalan Istana, Bandar
Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, Malaysia Diserahkan: 1 Disember
2013/Diterima: 2 Februari 2015 ABSTRACT This study was conducted to
study the morphological features of species under the genus Chaetoceros
isolated from the coastal waters of Pahang, Malaysia. The species
were isolated, cultivated and viewed under light microscope (LM)
and scanning electron microscope (SEM). Four taxa were successfully
isolated and cultivated in pure culture, namely Chaetoceros
affinis var. affinis, Chaetoceros affinis var.
willei, Chaetoceros anastomosans and Chaetoceros
baculites. Both varieties of C. affinis were considered
as distinct taxa where both strains can be distinguished based on
morphological characteristics. C. affinis var. affinis
has a thick cell wall compared with C. affinis var. willei.
C. anastomosans has special features including a silica bar
on the intersection of setae and the production of mucous. The length
of the aperture opening is a new record from this study. The new
record obtained for C. baculites includes the size of the
aperture, the terminal setae, spinal arrangement, the thinness of
the cell wall and the location of the species in tropical waters.
Detailed data on each species will be added to the taxonomic information
for future studies. Keywords: Chaetoceros;
coastal water; LM; morphology; SEM ABSTRAK Kajian ini dijalankan untuk
mengkaji ciri morfologi spesies di bawah genus Chaetoceros
yang diasingkan dari pesisir pantai Pahang, Malaysia. Kesemua
spesies dipencil, dikultur dan diperiksa melalui miskroskop cahaya
(LM)
dan mikroskop elektron imbasan (SEM). Empat takson telah berjaya
dipencilkan dan dikulturkan kepada kultur tulen, iaitu Chaetoceros
affinis var. affinis, Chaetoceros affinis var.
willei, Chaetoceros anastomosans, dan Chaetoceros
baculites. Kedua-dua varieti C. affinis dikategorikan
sebagai takson terdekat dengan kedua-dua strain boleh dibezakan
berdasarkan ciri morfologi. C. affinis var. affinis mempunyai
dinding sel yang tebal berbanding C. affinis var. willei.
C. anastomosans mempunyai ciri-ciri istimewa iaitu bar silika
di persilangan seta dan penghasilan mukus. Panjang bukaan merupakan
rekod terbaru di dalam kajian ini. Rekod terbaru diperoleh bagi
C. baculites yang merangkumi saiz bukaan, seta utama, susunan
duri dan kenipisan dinding sel dan spesies ini dijumpai di kawasan
tropika. Keperincian data untuk setiap spesies akan ditambah ke
dalam informasi taksonomi untuk kajian masa hadapan. Kata kunci: Chaetoceros; LM; morfologi;
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