Sains Malaysiana 45(10)(2016): 1517–1523

 

Evaluation of Antihyperglycemic and Antihyperlipidemic Potential of Nelumbo nucifera Seeds in Diabetic Rats

(Penilaian Potensi Antihiperglisemik dan Antihiperlipidemik Biji Nelumbo nucifera dalam Tikus Diabetis)

 

SHAHNAZ1, HIDAYATULLAH KHAN1*, FARMAN ALI2, AFZAL SHAH1, FARHAT KAMRAN3 & SARWAT JAHAN4

1Department of Chemistry, University of Science and Technology, Bannu (28100) KPK

Pakistan

2Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal, Dir (18000) KPK

Pakistan

 

3Department of Health Sciences, Liberty University, Lynchburg, VA, United States of America

 

4Department of Animal Sciences, Quaid-e-Azam University, Islamabad (41000), Pakistan

 

Diserahkan: 24 Januari 2016/Diterima: 6 Mac 2016

 

ABSTRACT

The present study was aimed at evaluating antihyperglycemic and antihyperlipidemic activity of nuciferin and norcoclaurine constituents of N. nucifera seeds, a well-known medicinal plant. The alloxan (100 mg/kg b.w) induced diabetic rats (200-250 g) were divided into seven groups (n = 6). Group I; normal control, Group II; diabetic control, Group III; standard, Group lV-VII were fed with methanolic crude extracts (100, 200 mg/kg), nuciferin and norcoclaurine (10 mg/kg b.w.), received for 15 days in dose dependent manner. The study included different parameters; examination of oral glucose, fasting blood glucose, serum lipid profile and checking for body weight changes. In oral glucose examination, within 60 and 80 min of treatment, extracts, nuciferin and norcoclaurine significantly reduced blood glucose (p<0.05) and restored body weight in diabetic rats. Alloxan- induced diabetic rats showed 30-50% reduction of blood glucose level (p<0.05) and recovered 5-20% body weight at day 15 after ingestion of crude extracts (100-200 mg/kg b.w.); and nuciferin and norcoclaurine (each at 10 mg/kg b.w.). It also recovered significantly elevated biochemical parameters such as triglycerides (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), total cholesterol (TC), serum urea and creatinine. Our findings indicated that N. nucifera seeds possess significant antihyperglycemic and antihyperlipidemic activity in diabetic rats.

 

Keywords: Antihyperglycemic; antihyperlipidemic; norcoclaurine; nuciferin; N. nucifera

 

ABSTRAK

Penyelidikan ini bertujuan untuk menilai aktiviti antihiperglisemik dan antihiperlipidemik juzuk nusiferin dan norkoklaurin biji N. nucifera, tumbuhan ubatan yang terkenal. Aloksan (100 mg/kg kW) tikus teraruh diabetis (200-250 g) dibahagikan kepada tujuh Kumpulan (n=6). Kumpulan I; kawalan biasa, Kumpulan II; kawalan diabetis, Kumpulan III; piawai, Kumpulan IV-VII diberi makan dengan ekstrak mentah metanolik (100, 200 mg/kg), nusiferin dan norkoklaurin (10 mg/kg b.w.), menerima dos secara bersandar selama 15 hari. Kajian ini merangkumi parameter yang berbeza; pemeriksaan glukosa secara oral, glukosa darah puasa, profil lipid serum dan pemeriksaan perubahan berat badan. Dalam tempoh 60 dan 80 min rawatan untuk pemeriksaan glukosa secara oral, ekstrak, nusiferin dan norkoklaurin telah mengurangkan glukosa darah (p<0.05) dan memulihkan berat badan tikus diabetis. Aloksan - tikus teraruh diabetis menunjukkan 30-50% pengurangan paras glukosa darah (p<0.05) dan mendapat kembali 5-20% berat badan pada hari ke-15 selepas ketakhadaman ekstrak mentah (100-200 mg/kg b.w.); serta nusiferin dan norkoklaurin (masing-masing pada 10 mg/kg b.w.). Ia juga dengan ketara mendapat kembali peningkatan parameter biokimia seperti trigliserida (TG), lipoprotein ketumpatan rendah (LDL), lipoprotein ketumpatan tinggi (HDL), jumlah kolesterol (TC), serum urea dan kreatinina. Keputusan kajian kami menunjukkan bahawa benih N. nucifera mempunyai aktiviti antihiperglisemik dan antihiperlipidemik yang penting dalam tikus diabetis.

 

Kata kunci: Antihiperglisemik; antihiperlipidemik; norkoklaurin; nusiferin; N. nucifera

RUJUKAN

Ayber, M.J., Riera, A.N., Grau, A. & Sanchhez, S.S. 2008. Hypoglycemic effect of Smallanthus soncitolius water extract of leaves in normal and diabetic rats. Journal of Ethnopharmacology 74: 125-132.

Boyle, J.P., Honeycutt, A.A., Narayan, K.M., Hoerger, T.J., Geiss, L.S., Chen, H. & Thompson, T.J. 2001. Projection of diabetes burden through 2050: Impact of changing demography and disease prevalence in the US. Diabetes Care 24: 1936-1940.

Chatterjea, M.N. & Shinde, R. 2002. Text Book of Medical Biochemistry. New Delhi: Jaypee Brothers Medical Publishers. p. 317.

Dash, G.K., Patro, C.H.P. & Maiti, A.K. 2005. A study on the anti-hyperglycemic effect of Premna corymbosa Rottl. roots. Journal of Natural Remedy 5: 31-34.

Fowler, M.J. 2007. Diabetes treatment, Part 2: Oral agents for glycemic management. Clinical Diabetes 25: 131-134.

Haldar, P.K., Kar, B., Bhattacharya, S., Bala, A. & Kumar, R.B.S. 2010. Antidiabetic activity and modulation of antioxidant status by Sansevieria roxburghiana rhizome in Streptozotocin-induced diabetic rats. Diabetologia Croatica 39(4): 115-123.

Howard, B.V. 1987. Lipoprotein metabolism in diabetes mellitus. The Journal of Lipid Research 28: 613-628.

Huang, B., Ban, X.Q., He, J.S., Tong, J., Tian, J. & Wang, Y.W. 2010. Hepatoprotective and antioxidant activity of ethanolic extracts of edible lotus (Nelumbo nucifera Gaertn.) leaves. Food Chemistry 120: 8738-8778.

Kumar, S.A., Vanitha, S., Ganesh, M., Jayaprakash, J. & Ramaswamy, N.M. 2010. Hypolipidemic activity of Sesbania grandiflora in triton wr-1339 induced hyperlipidemic rats. International Journal of Phytomedicine 2: 52-58.

Leontowicz, H., Gorinstein, S., Lojek, A., Leontowicz, M., Ciz, M. & Soliva-Fortuny, R. 2002. Comparative content of some bioactive compounds in apples, peaches and pears and their influence on lipids and antioxidant capacity in rats. The Journal of Nutritional Biochemistry 13: 603-610.

Ma, C., Wang, J., Chu, H., Zhang, X., Wang, Z., Wang, H. & Li, G. 2014. Purification and characterization of aporphine alkaloids from leaves of Nelumbo nucifera Gaertn and their effects on glucose consumption in 3T3-L1 adipocytes. International Journal of Molecular Science 15(3): 3481-3494.

Maiti, R., Jana, D., Das, U.K. & Ghosh, D. 2004. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology 92: 85-91.

Marlos, R.J. & Farnsworth, N.R. 1995. Antidiabetic plants and their active constituents. Phytomedicine 2: 137-189.

Mooradian, A.D. 2009. Dyslipidemia in type 2 diabetes mellitus. Nature Clinical Practice Endocrinology and Metabolism 5(3): 150-159.

Mooradian, A.D., Haas, M.J., Wehmeier, K.R. & Wong, N.C. 2008. Obesity-related changes in high density lipoprotein metabolism. Obesity 16: 1152-1160.

Mukherjee, P.K., Mukherjee, D., Maji, A.K., Rai, S. & Heinrich, M. 2009. The sacred lotus (Nelumbo nucifera) - phytochemical and therapeutic profile. Journal of Pharmacy and Pharmacology 61(4): 407-422.

Nagappa, A.N., Thakurdesai, P.A., Venkat Rao, N. & Sing, J. 2003. Antidiabetic activity of Terminalia catappa Linn. fruits. Journal of Ethnopharmacology 88: 45-50.

Prince, S.M. & Menon, V.P. 2000. Hypoglycemic and other related actions of Tinospora cardifolia roots in alloxan induced diabetic rats. Journal of Ethnopharmacology 70: 9-15.

Sharma, S.R., Dwivedi, S.K. & Swarup, D. 1997. Hypoglycemic, antihyperglycemic and hypolipidemic activity of Caesalpinia bonducella seeds in rats. Journal of Ethnopharmacology 58: 39-44.

Sindhu, R.K., Vasudeva, N. & Sharma, K. 2011. Hypolipidemic potential of ethanolic extract of Verbesina encelioides Benth. roots. Acta Poloniae Pharmaceutica - Drug Research 68(2): 301-304.

Sridhar, K.R. & Bhat, R. 2007. Lotus - a potential nutraceutical source. Journal of Agricultural Science and Technology 3(1): 143-155.

Szkudelski, T. 2001. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research 50: 537-546.

Temme, E.H., Vaqn, H.P.G., Schouten, F.G. & Kesteloot, H. 2002. Effect of plant sterol enriched spread on serum lipids and lipoproteins in middly hypercholesterolemia subjects. Acta Cardiologica 57: 111-115.

Yada, S.B., Tripathi, V., Singh, P.K. & Pandey, H.P. 2000. Antioxidant activity of Cuscuta reflexa stem. Indian Journal of Pharmaceutical Science 62: 477-478.

 

 

*Pengarang untuk surat-menyurat; email: hidayat_sks@yahoo.com

 

 

 

 

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