Sains Malaysiana 46(10)(2017): 1807–1816

http://dx.doi.org/10.17576/jsm-2017-4610-18

 

Kesan Kaedah Pemendakan Berbeza terhadap Ciri Fizikokimia dan Aktiviti Antioksidan Alginat daripada Sargassum sp.

(Effects of Different Precipitation Method on Physicochemical Properties and Antioxidant Activities of Alginates from Sargassum sp.)

 

SZE LING LIM, SHARIFAH HABIBAH SYED KHALAFU, WAN AIDA WAN MUSTAPHA & SENG JOE LIM*

 

Pusat Pengajian Sains Kimia dan Teknologi Makanan, Fakulti Sains dan Teknologi Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 9 Mac 2017/Diterima: 4 Mei 2017

 

ABSTRAK

Alginat merupakan polisakarida berstruktur anionik yang terdapat dalam matriks alga perang dan lazimnya digunakan sebagai agen penggelan, pengemulsi dan penstabil dalam industri makanan. Objektif utama kajian dijalankan adalah untuk mengenal pasti kesan kaedah pemendakan berbeza terhadap ciri fizikokimia alginat. Dalam kajian ini, alginat telah diekstrak daripada Sargassum sp. dengan menggunakan dua kaedah pemendakan yang berbeza iaitu melalui pemendakan dengan garam kalsium dan asid (sampel A) dan juga pemendakan dengan asid dan etanol (sampel B). Analisis ATR-FTIR telah mengesahkan kehadiran alginat dalam kedua-dua ekstrakSargassum sp. Hasil pengekstrakan alginat bagi sampel A dan sampel B masing-masing adalah 28.16% dan 18.24%. Ciri fizikokimia yang diuji ialah warna, kelikatan, aktiviti penggelan dan kuasa pemerangkapan radikal bebas DPPH dan dibandingkan dengan alginat komersial (Sigma-Aldrich). Alginat yang terhasil adalah berwarna kuning kecoklatan dan mempunyai kelikatan pseudoplastik dengan penipisan ricih. Aktiviti penggelan kedua-dua sampel (A & B) adalah berbeza secara signifikan, (p<0.05) dengan alginat komersial. Kuasa pemerangkapan radikal bebas DPPH sampel A adalah lebih tinggi berbanding alginat komersial (20.10 ±3.84 & 11.89 ±3.12). Secara keseluruhan, kaedah pemendakan sampel A adalah lebih baik berbanding sampel B disebabkan oleh hasil alginat yang tinggi, aktiviti antioksidan yang tinggi dan ciri fizikokimia yang hampir setanding dengan alginat komersial.

 

Kata kunci: Alginat; antioksidan; fizikokimia; kaedah pemendakan; Sargassum sp.

 

ABSTRACT

Alginate is an anionic structural polysaccharide that is found in the matrix of brown algae and normaly used as gelling, emulsifying and stabilizing agents in food industry. The aim of this research was to determine the effects of different alginate precipitation methods towards its physicochemical properties and antioxidant activity. In this research, alginate was extracted from Sargassum sp. using two different precipitation methods, where alginates were precipitated using calcium salt and acid (sample A), and also precipitated using acid and ethanol (sample B). The extraction yield of alginates were 28.16% and 18.24% for sample A and sample B, respectively. ATR-FTIR analysis showed that the polysaccharides extracted from Sargassum sp. were indeed alginates. The physicochemical characteristics determined were colour, viscosity, gelling activity and free radical DPPH scavenging activity and were compared with commercial alginate (Sigma-Aldrich). Extracted alginates were yellow brownish in colour and have pseudoplastic viscosity with sheer thinning behaviour. Gelling activities of both sample (A & B) were significantly different, (p<0.05) compared to that of commercial alginate. Meanwhile, the scavenging activity of DPPH free radical in sample A was higher than commercial alginate (20.10 ±3.84 & 11.89 ±3.12). Overall, precipitation method of sample A was better than sample B due to higher yield of alginate, higher antioxidant activity and almost similar physicochemical properties to commercial alginate.

 

Keywords: Alginate; antioxidant; physicochemical; precipitation method; Sargassum sp.

RUJUKAN

Andriamanantoanina, H. & Rinaudo, M. 2010. Charaterization of the alginates from five Madagascan brown algae. Carbohydrate Polymers 82(3): 555-560.

Cong, Q., Xiao, F., Liao, W., Dong, Q. & Ding, K. 2014. Structure and biological activities of an alginate from Sargassum fusiforme, and its sulfated derivative. International Journal of Biological Macromolecules 69: 252-259.

Davis, T.A., Ramirez, M., Mucci, A. & Larsen, B. 2004. Extraction, isolation and cadmium binding of alginate from Sargassum spp. Journal of Applied Phycology 16(4): 275- 284.

Draget, K.I., Strand, B., Hartmann, M., Valla, S., Smidsrød, O. & Skjå-Brӕk, G. 2000. Ionic and acid gel formation of epimerised alginates: The effect of algE4. International Journal of Biological Macromolecules 27(2): 117-122.

Fenoradosoa, T.A., Ali, G., Dellattre, C., Laroche, C., Petit, E., Wadouachi, A. & Michaud, P. 2010. Extraction and characterization of an alginate from the brown seaweed Sargassum turbinarioides Grunow. Journal of Applied Phycology 22(2): 131-137.

Gómez-Ordóñez, E. & Rupérez, P. 2011. FTIR-ATR spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds. Food Hydrocolloids 25: 1514-1520.

Gomez, C.G., Lambrecht, M.V.P., Lozanoa, J.E., Rinaudo, M. & Villar, M.A. 2009. Influence of the extraction-purification conditions on final properties of alginates obtained from brown algae (Macrocystis pyrifera). International Journal of Biological Macromolecules 44(4): 365-371.

Ho, Y.M., Wan Amir Nizam, W.A. & Wan Rosli, W.I. 2016. Aktiviti antioksida dan kandungan polifenolik bagi varieti berbeza jagung muda dan sutera jagung Malaysia). Sains Malaysiana 45(2): 195-200.

Kelishomi, Z.H., Goliaei, B., Mahdavi, H., Nikoofar, A., Rahimi, M., Movahedi, A.A.M., Mamashli, F. & Bidgeli, B. 2016. Antioxidant activity of low molecular weight alginate produced by thermal treatment. Food Chemistry 196: 897- 902.

Leal, D., Matsuhiro, B., Rossib, M. & Caruso, F. 2008. FT-IR spectra of alginic acid block fractions in three species of brown seaweeds. Carbohydrate Research 343(2): 308-316.

Lencina, M.M.S., Andreucetti, N.A., Gómez, C.G. & Villar, M.A. 2012. Recent studies on alginates based blends, composites, and nanocomposites. Dlm Advances in Natural Polymers, disunting oleh Thomas, S., Visakh, P.M. & Mathew, A.P. New York: Springer-Verlag Berlin Heidelberg. Hlm. 193-254.

Lim, S.J., Aida, W.M.W., Maskat, M.Y., Mamot, S., Ropien, J. & Mohd, D.M. 2014. Isolation and antioxidant capacity of fucoidan from selected Malaysian seaweeds. Food Hydrocolloids 42: 280-288.

McHugh, D.J. 1987. Production and Utilization of Products from Commercial Seaweeds- FAO Fisheries Techical Paper 288. Rome: Food and Agriculture Organization of the United Nations. (http://www.fao.org/3/ax5822e04.htm#chapter 2 production, properties and uses of alginates).

Mirshafiey, A. & Rehm, B.H.A. 2009. Alginate and its comonomer mannuronic acid: Medical relevance as drugs. Dlm Alginates: Biology and Applications, disunting oleh Rehm, B.H.A. Berlin: Springer-Verlag. hlm. 229-260.

Mohd Fadzelly, A.B., Fifilyana, A.K. & Perisamy, E. 2015. Comparison of phytochemicals and antioxidant properties of different fruit parts of selected Artocarpus species from Sabah, Malaysia. Sains Malaysiana44(3): 355-363.

Mohamad Latifi, A., Nejad, E.S. & Babavalian, H. 2015. Comparison of extraction different methods of sodium alginate from brown alga Sargassum sp. localized in the Southern of Iran. Journal of Applied Biotechnology 2(2): 251-255.

Mushollaeni, W. 2011. The physicochemical characteristics of sodium alginate from Indonesian brown seaweeds. African Journal of Food Science 5(6): 349-352.

Niekraszewicz, B. & Niekraszewicz, A. 2009. The structure of alginate, chitin and chitosan fibres. Dlm Handbook of Textile Fibre Structure. Volume 2: Woodhead Publishing Series in Textiles, disunting oleh Eichhorn, S.J., Hearle, W.S., Jeffe, M. & Kukutani, T. London: Woodhead Publishing Limited. Hlm. 266-304.

Pawar, S.N. & Edgar, K.J. 2012. Alginate derivatization: A review of chemistry, properties and applications. Biomaterials 33(11): 3279-3305.

Remminghorst, U. 2007. Polymerization and export of alginate in Pseudomonas aeruginosa: Functional assignment and catalytic mechanism of Alg8/44. Tesis Doctor of Philosophy in Microbiology, Massey University (tidak diterbitkan).

Rioux, L.E., Turgeon, S.L. & Beaulieu, M. 2007. Characterization of polysaccharides extracted from brown seaweeds. Carbohydrate Polymers 69(3): 530-537.

Sari-Chmayssem, N., Taha, S., Mawlawi, H., Guégan, J.P., Jeftić, J. & Benvegnu, T. 2015. Extracted and depolymerization alginates from brown algae Sargassum vulgare of Lebanese origin: Chemical, rheological, and antioxidant properties. Journal of Applied Phycology 28(3): 1915-1929.

Sellimi, S., Younes, I., Ayed, H.B., Maalej, H., Montero, V., Rinaudo, M., Dahia, M., Mechichi, T., Hajji, M. & Nasri, M. 2014. Structural, physicochemical and antioxidant properties of sodium alginate isolated from a Tunisian brown seaweed. International Journal of Biological Macromolecules 72: 1358-1367.

Silverstein, R.M. & Webster, F.X. 1998. Spectrometric Identification of Organic Compounds 6th ed. New York: John Wiley & Sons, Inc.

Suresh Kumar, K., Ganesan, K. & Subba Rao, P.V. 2008. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (doty) Doty- an edible seaweed. Food Chemistry 107(1): 289-295.

Torres, M.R., Sousa, A.P.A., Silva Filho, E.A.T., Melo, D.F., Feitosa, J.P.A., De Paula, R.C.M. & Lima, M.G.S. 2007. Extraction and physicochemical characterization of Sargassum vulgare alginate from Brazil. Carbohydrate Research 324(14): 2067-2074.

Vauchel, P., Leroux, K., Kaas, R., Arhaliass, A., Baron, R. & Legrand, J. 2009. Kinetics modelling of alginate alkaline extraction from Laminaria digitata. Bioresource Technology 100(3): 1291-1296.

Venkatesan, J. & Kim, S.K. 2015. Marine algae derived polysaccharides for bone tissue regeneration. Dlm. Marine Algae Extracts: Procesess, Products, and Applications, disunting oleh Kim, S.K. & Chojnacka, K. New Jersey: John Wiley & Sons. Hlm. 509-522.

Yabur, R., Bashan, Y. & Carmona, G.V. 2007. Alginate from the macroalgae Sargassum sinicolaas a novel source for microbial immobilization material in wastewater treatment and plant growth promotion. Journal of Applied Phycology 19(1): 43-53.

 

 

*Pengarang untuk surat-menyurat; email: joe@ukm.edu.my

 

 

 

 

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