Sains Malaysiana 46(4)(2017): 521–528

http://dx.doi.org/10.17576/jsm-2017-4604-02

Analysis of Simple Sequence Repeat Markers Linked to Submergence Tolerance on Newly Developed Rice Lines Derived from MR263 × Swarna-Sub1

(Analisis Penanda Ulangan Jujukan Ringkas terhadap Titisan Padi Baharu Dibangunkan yang Toleransi Tenggelam daripada Kacukan MR263 × Swarna -Sub1)

WELLAND COSMAS MOJULAT¹, MOHD RAFII YUSOP^1,2*, MOHD RAZI ISMAIL^1,2, ABDUL SHUKOR JURAIMI¹, ABDUL RAHIM HARUN³, FAHIM AHMED¹, FATAH ABRO TANWEER^1,2 & MD. ABDUL LATIF⁵

¹Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

²Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

³Agrotechnology and Bioscience Division, Malaysian Nuclear Agency, Bangi

43000 Kajang, Selangor Darul Ehsan, Malaysia

⁴Department of Plant Breeding and Genetics, Faculty of Crop Production, Sindh Agriculture University Tandojam, Sindh, Pakistan

⁵Bangladesh Rice Research Institute (BRRI), Gazipur, Dhaka, Bangladesh

Diserahkan: 12 Januari 2016/Diterima: 24 September 2016

ABSTRACT

Nowadays, in extreme changing environments, development of submergence tolerance variety is necessary for ensuring crop production stability where, it is known that Malaysian commercial rice varieties such as MR219, MR220 and MR263 were severely susceptible to submergence. First step towards the development of submergence tolerance variety starts with the breeding program by crossing MR263 and Swarna-Sub1. Marker-assisted selection (MAS) was carried out through the utilization of simple sequence repeats (SSR) markers, considering its reliability as pre-selection tools to conduct this research. F1 generations plants were confirmed by tightly linked markers. In case of background study, out of 180 SSR markers, 38 were found polymorphic between two parents. Association of molecular markers and submergence tolerance were determined using Chi-square test. MR263 × Swarna-Sub1 F2 lines were tested for Sub1 gene conformation using the markers RM8300 and RM219. These markers showed a good fit to the expected marker segregation ratio (1:2:1) in a Mendelian single gene model (DF=1.0, p≤0.05). Eleven homozygous lines with Sub1 gene out of 256 were selected for future development of submergence tolerant varieties. Eleven lines were selected based on phenotypic study and agronomic performance.

Keywords: Marker-assisted selection; rice; Sub1; submergence tolerance; SSR

ABSTRAK

Pembangunan varieti padi yang toleransi tenggelam adalah penting bagi memastikan kestabilan pengeluaran tanaman akibat perubahan melampau alam sekitar yang tidak menentu. Varieti padi komersial Malaysia seperti MR219, MR220 dan MR263 telah dikenal pasti terjejas teruk akibat tenggelam. Langkah pertama ke arah pembangunan varieti yang toleransi tenggelam bermula dengan program pembiakbakaan MR263 dan Swarna-Sub1. Dalam kajian ini, pemilihan berbantukan penanda (MAS) telah dijalankan dengan menggunakan penanda ulangan jujukan ringkas (SSR) yang disahkan kebolehpercayannya sebagai alat pra-pemilih. Pokok generasi F1 telah disahkan oleh penanda yang terkait erat. Dalam kajian latar belakang, daripada 180 penanda SSR, 38 penanda adalah polimorfik antara kedua-dua induk. Hubungan antara penanda ulangan jujukan ringkas dan toleransi tenggelam ditentukan dengan menggunakan ujian Chi-square. Titisan F2 MR263 × Swarna-Sub1 telah diuji untuk korformasi gen Sub1 dengan menggunakan penanda RM8300 dan RM219. Penanda ini menunjukkan keserasian yang baik dengan kadar segregasi yang dijangka (1:2:1) dalam model gen tunggal Mendel (DF=1.0, p≤0.05). Sebelas titisan homozigot yang mempunyai gen Sub1 telah dipilih daripada 256 titisan untuk pembangunan masa depan varieti padi yang toleransi tenggelam. Sebelas titisan ini dipilih berdasarkan kajian fenotip dan prestasi agronomi.

Kata kunci: Padi; pemilihan berbantukan penanda; Sub1; toleransi tenggelam; SSR

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*Pengarang untuk surat menyurat; email: mrafii@upm.edu.my