Sains Malaysiana 46(8)(2017): 1279–1284

http://dx.doi.org/10.17576/jsm-2017-4608-13

 

Antibacterial Activity of Alkaloid Extracts from Ochrosia oppositifolia

(Aktiviti Antibakteria Ekstrak Alkaloid daripada Ochrosia oppositifolia)

 

PUTERI I.A.M. MAHMUD1*, W.A. YAACOB1 & NAZLINA IBRAHIM2

 

1School of Chemical Science and Food Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

2School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 27 Julai 2016/Diterima: 3 Januari 2017

 

ABSTRACT

Ochrosia oppositifolia can be found along the coastline and is locally known as 'Mempelam pasih' or 'Mangga laut'. In this study, the alkaloids from the leaves, stem-bark and roots were extracted and tested for their antibacterial activity. So far, no previous studies have been carried out to determine the antibacterial potential of the extracts. Each extract was tested using disc-diffusion and minimum inhibitory concentration (MIC) methods. The highest inhibitory diameters shown by 10 mg/mL extracts of the leaves and stem-bark against Staphylococcus aureus and the roots against MRSA were 10.0±2.8 and 10.5±2.1 and 14.0±2.8 mm. On the other hand, the MIC values exhibited by the leaf and stem-bark extracts against Bacillus subtilis, Salmonella thyphimurium and Serratia marcescens and the root extract against Vibrio fluvialis were 3.75 and 0.94 and 0.12 mg/mL. This study broadens the bioactivity potential of the plant and the information obtained can be utilized for pharmacological purposes.

 

Keywords: Alkaloid extract; antibacterial activity; disc diffusion; minimum inhibitory concentration; Ochrosia oppositifolia

 

ABSTRAK

Ochrosia oppositifolia adalah tumbuhan pesisir pantai dan dikenali orang tempatan sebagai 'Mempelam pasih' atau 'Mangga laut'. Dalam kajian ini, alkaloid daripada daun, kulit dan akar diekstrak dan diuji aktiviti antibakteria mereka. Tiada kajian terdahulu yang dijalankan setakat ini bagi menentukan potensi aktiviti ekstrak tersebut. Setiap ekstrak diuji menggunakan kaedah resapan-cakera dan kepekatan perencatan minimum (MIC). Diameter perencatan tertinggi ditunjukkan oleh ekstrak 10 mg/mL bagi daun dan kulit terhadap Staphylococcus aureus dan akar terhadap SARM adalah 10.0±2.8 dan 10.5±2.1 dan 14.0±2.8 mm. Selain itu, nilai MIC yang diperlihatkan oleh ekstrak daun dan kulit terhadapBacillus subtilis, Salmonella thyphimurium dan Serratia marcescens serta ekstrak akar terhadapVibrio fluvialis adalah 3.75 dan 0.94 dan 0.12 mg/mL. Kajian ini memperluaskan potensi bioaktiviti tumbuhan tersebut dan maklumat yang diperoleh dapat digunakan bagi tujuan farmakologi.

 

Kata kunci: Aktiviti antibakteria; ekstrak alkaloid; kepekatan perencatan minimum; Ochrosia oppositifolia; resapan-cakera

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*Pengarang untuk surat-menyurat; email: put3ili@gmail.com

 

 

 

 

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