Sains Malaysiana 47(12)(2018): 3003–3008

http://dx.doi.org/10.17576/jsm-2018-4712-09

 

Arabidopsis AT2G02870 Loss of Function Mutants Lead to Enhanced Production of Hydroperoxide Lyase Pathway Genes and Products

(Mutan Fungsi-Hilang Arabidopsis AT2G02870 Membawa kepada Peningkatan Gen

dan Produk Tapak Jalan Hidroperoksida Liase)

MUHAMMAD NAEEM-UL-HASSAN^1,3, ZAMRI ZAINAL^1,2, NUR ATHIRAH ABD HAMID²,

MUHAMMAD SAJAD^2,4 & ISMANIZAN ISMAIL^1,2*

 

¹Centre for Biotechnology and Functional Food, Faculty of Science and Technology, University Kebangsaan Malaysia, 46300 Bangi UKM, Selangor Darul Ehsan, Malaysia

 

²Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 46300 Bangi UKM, Selangor Darul Ehsan, Malaysia

 

³Department of Chemistry, University of Sargodha, Sargodha, Pakistan

 

⁴Department of Plant Breeding and Genetics, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Pakistan

 

Diserahkan: 4 September 2018 /Diterima: 19 September 2018

 

ABSTRACT

F-box proteins containing variable C-terminal domains make an essential part of SKP1-Cullin-Ring box-F box (SCF) complex. SCF complex catalyzes the final step to link the ubiquitin tag with the target protein, destined for degradation, through F-box protein that confer overall substrate specificity to the complex. In this study, we analyzed the role of At2g02870, a Kelch containing F-box protein from Arabidopsis thaliana, by using reverse genetics strategy. At2g02870 loss of function mutant lines (at2g02870) were analyzed and compared with wild type plants for the expression of genes and products of hydroperoxide lyase (HPL) branch of oxylipin pathway. We found that the at2g02870 plants have enhanced expression of HPL pathway genes and produce more green leaf volatiles (GLV) than the wild type plants. Our results suggested that the gene is involved in the regulation of HPL pathway, possibly through the degradation of enzymes or/and the regulatory factors of the pathway.

 

Keywords: F-box proteins; hydroperoxide lyase; Kelch-repeat; lipoxygenase; oxylipin

 

ABSTRAK

Protein kotak F mengandungi pelbagai domain C-terminal yang membentuk bahagian penting kompleks SKP-Cullin-Ring box-F box (SCF). Kompleks SCF memangkinkan peringkat akhir pengikatan tag ubiquitin dengan protein sasaran untuk degradasi, melalui protin kotak F yang memberikan kespesifikan substrak secara keseluruhan kepada kompleks. Dalam kajian ini, kami menganalisis peranan At2g02870, protein kotak F yang mengandungi Kelch dari Arabidopsis thaliana menggunakan strategi genetik berbalik. Titisan mutan kehilangan fungsi At2g02870 telah dianalisis dan dibandingkan dengan tumbuhan jenis liar untuk pengekpsresan gen dan produk cabang hidroksiperoksida liase (HPL) tapak jalan oksilipin. Kami dapati tumbuhan at2g02870 telah meningkatkan pengekspresan gen tapak jalan HPL dan menghasilkan lebih banyak bahan meruap daun hijau (GLV) berbanding dengan tumbuhan jenis liar. Keputusan kami menunjukkan gen tersebut terlibat dalam pengawalan tapak jalan HPL, kemungkinan melalui degradasi enzim atau/dan faktor pengawalan tapak jalan tersebut.

 

Kata kunci: Hidroperoksida liase; Kelch-berulang; lipoksigenase; oksilipin; protein kotak F

RUJUKAN

Alonso, J.M. & Ecker, J.R. 2006. Moving forward in reverse: Genetic technologies to enable genome-wide phenomic screens in Arabidopsis. Nature Reviews Genetics 7(7): 524.

del Pozo, J.C. & Estelle, M. 2000. F-box proteins and protein degradation: An emerging theme in cellular regulation. Plant Molecular Biology 44(2): 123-128.

Deshaies, R. 1999. SCF and cullin/ring h2-based ubiquitin ligases. Annual Review of Cell and Developmental Biology 15(1): 435-467.

Griffiths, G. 2015. Biosynthesis and analysis of plant oxylipins. Free Radical Research 49(5): 565-582.

Hassan, M.N., Zainal, Z. & Ismail, I. 2015a. Green leaf volatiles: Biosynthesis, biological functions and their applications in biotechnology. Plant Biotechnology Journal 13(6): 727-739.

Hassan, M.N.U., Zainal, Z. & Ismail, I. 2015b. Plant Kelch containing f-box proteins. RSC Advances 5(53): 42808- 42814.

Hershko, A. 1998. The ubiquitin system. In Ubiquitin and The Biology of the Cell, edited by Peters, J.M., Harris, J.R. & Finley, D. Boston, MA: Springer. pp. 1-17.

Hong, S.Y., Kim, O.K., Kim, S.G., Yang, M.S. & Park, C.M. 2010. Nuclear import and DNA binding of the zhd5 transcription factor is modulated by a competitive peptide inhibitor in Arabidopsis. Journal of Biological Chemistry M110. 167692.

Kim, H.J., Chiang, Y.H., Kieber, J.J. & Schaller, G.E. 2013. SCF(kmd) controls cytokinin signaling by regulating the degradation of type-b response regulators. Proceedings of the National Academy of Sciences of The United States of America 110(24): 10028-10033.

Kuroda, H., Takahashi, N., Shimada, H., Seki, M., Shinozaki, K. & Matsui, M. 2002. Classification and expression analysis of Arabidopsis f-box-containing protein genes. Plant & Cell Physiology 43(10): 1073-1085.

Li, M., Tang, D., Wang, K., Wu, X., Lu, L., Yu, H., Gu, M., Yan, C. & Cheng, Z. 2011. Mutations in the f‐box gene larger panicle improve the panicle architecture and enhance the grain yield in rice. Plant Biotechnology Journal 9(9): 1002-1013.

Li, X., Zhang, D., Hannink, M. & Beamer, L.J. 2004. Crystal structure of the Kelch domain of human keap1. Journal of Biological Chemistry 279(52): 54750-54758.

Li, Y., Liu, Z., Wang, J., Li, X. & Yang, Y. 2016. The Arabidopsis Kelch repeat f-box e3 ligase arkp1 plays a positive role for the regulation of abscisic acid signaling. Plant Molecular Biology Reporter 34(3): 582-591.

Marino, D., Peeters, N. & Rivas, S. 2012. Ubiquitination during plant immune signaling. Plant Physiology 160(1): 15-27.

Matsui, K., Sugimoto, K., Mano, J.I., Ozawa, R. & Takabayashi, J. 2012. Differential metabolisms of green leaf volatiles in injured and intact parts of a wounded leaf meet distinct ecophysiological requirements. PLoS One 7(4): e36433.

Naeem-ul-Hassan, M., Zainal, Z., Kiat, C.J., Monfared, H.H. & Ismail, I. 2017. Arabidopsis thaliana skp1 interacting protein 11 (at2g02870) negatively regulates the release of green leaf volatiles. RSC Advances 7(88): 55725-55733.

Oono, Y., Seki, M., Nanjo, T., Narusaka, M., Fujita, M., Satoh, R., Satou, M., Sakurai, T., Ishida, J. & Akiyama, K. 2003. Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca. 7000 full‐length cdna microarray. The Plant Journal 34(6): 868-887.

Parinov, S., Sevugan, M., Ye, D., Yang, W.C., Kumaran, M. & Sundaresan, V. 1999. Analysis of flanking sequences from dissociation insertion lines: A database for reverse genetics in Arabidopsis. The Plant Cell 11(12): 2263-2270.

Shao, T., Qian, Q., Tang, D., Chen, J., Li, M., Cheng, Z. & Luo, Q. 2012. A novel gene ibf1 is required for the inhibition of brown pigment deposition in rice hull furrows. Theoretical and Applied Genetics 125(2): 381-390.

Zhang, X., Gou, M. & Liu, C.J. 2013. Arabidopsis Kelch repeat f-box proteins regulate phenylpropanoid biosynthesis via controlling the turnover of phenylalanine ammonia-lyase. Plant Cell 25(12): 4994-5010.

Zhang, X., Gou, M., Guo, C., Yang, H. & Liu, C.J. 2015. Down-regulation of Kelch domain-containing f-box protein in Arabidopsis enhances the production of (poly) phenols and tolerance to ultraviolet radiation. Plant Physiology 167(2): 337-350.

 

*Pengarang untuk surat-menyurat; email: maniz@ukm.edu.my